FastQCFastQC Report
Wed 25 May 2016
SRR1779743_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779743_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences220588
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAC509.767973E-1055.9961910
TAGATCT2500.054.59628739
AGATCTC2550.053.52577240
CTAGAGT200.006946495352.4964269
AGTATCG200.006946495352.49642635
TCTCGGT2550.049.40840543
TCGGTGG2500.047.59676445
CGCCGTA2000.047.24678453
GCCGTAT2300.045.6490754
CCTACGA1850.045.40231721
CGGTGTT851.8189894E-1245.29103546
GTATCAT1700.045.29103557
GTAGATC1950.044.86874438
CGGTGGT2900.044.65213846
CGTATCA1900.044.2075256
CTCGGTG2800.043.74702544
CTCGTTG802.910383E-1143.7470244
GGTGGTC2600.043.0739947
CCGTATC2450.042.85422555
GATCTCT1150.042.605841