##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779743_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	220588
Sequences flagged as poor quality	0
Sequence length	76
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.485833318222205	33.0	23.0	34.0	12.0	34.0
2	27.262725080240084	33.0	23.0	34.0	12.0	34.0
3	26.26480588245961	32.0	21.0	34.0	11.0	34.0
4	28.241536257638675	33.0	23.0	34.0	12.0	34.0
5	27.97459063956335	34.0	23.0	34.0	11.0	34.0
6	30.00384880410539	36.0	24.0	38.0	11.0	38.0
7	31.021057355794515	36.0	27.0	38.0	12.0	38.0
8	30.222056503526936	36.0	25.0	38.0	11.0	38.0
9	31.680014325348612	37.0	27.0	38.0	12.0	38.0
10-11	31.32555714726096	37.0	27.0	38.0	11.0	38.0
12-13	30.584249823199812	35.5	25.5	38.0	11.0	38.0
14-15	30.37619680127659	35.5	25.0	38.0	11.0	38.0
16-17	30.148664025241626	35.5	24.0	38.0	11.0	38.0
18-19	30.74312292599779	36.0	26.0	38.0	11.0	38.0
20-21	29.650835494224527	35.0	23.5	38.0	11.0	38.0
22-23	29.694158340435564	35.5	24.0	38.0	11.0	38.0
24-25	30.62271293089379	36.0	25.0	38.0	11.0	38.0
26-27	29.361776252561334	35.0	22.0	38.0	11.0	38.0
28-29	31.159009102943042	37.0	26.5	38.0	11.0	38.0
30-31	31.611760385877744	37.0	27.0	38.0	11.0	38.0
32-33	31.704968085299292	37.0	27.0	38.0	11.0	38.0
34-35	31.753599470506103	37.0	27.0	38.0	11.0	38.0
36-37	30.008642809218998	35.0	24.0	38.0	11.0	38.0
38-39	28.555730592779298	34.0	21.5	37.5	11.0	38.0
40-41	29.40035269370954	34.5	23.0	37.5	11.0	38.0
42-43	30.325124213465827	35.5	25.0	38.0	11.0	38.0
44-45	31.808647795890984	37.0	27.0	38.0	11.0	38.0
46-47	30.555846192902607	35.5	26.0	38.0	11.0	38.0
48-49	30.023838558761128	35.5	23.5	38.0	11.0	38.0
50-51	28.129361071318478	33.5	21.0	37.5	11.0	38.0
52-53	30.956545686982068	36.5	26.0	38.0	11.0	38.0
54-55	30.164338041960576	35.5	24.0	38.0	11.0	38.0
56-57	30.57327007815475	36.5	25.0	38.0	11.0	38.0
58-59	31.48976145574555	37.0	27.0	38.0	11.0	38.0
60-61	31.950663680707926	37.0	28.5	38.0	11.0	38.0
62-63	31.860062650733493	37.0	29.0	38.0	11.0	38.0
64-65	31.614292255245072	37.5	28.5	38.0	11.0	38.0
66-67	29.793379512938145	35.5	23.5	38.0	11.0	38.0
68-69	29.84690010336011	35.5	23.5	38.0	11.0	38.0
70-71	29.501718135166012	35.0	23.0	38.0	11.0	38.0
72-73	28.47432543928047	34.0	21.5	37.5	11.0	38.0
74-75	30.215127296135783	35.5	24.0	38.0	11.0	38.0
76	30.5485747184797	36.0	25.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	13.0
4	3.0
5	3.0
6	2.0
7	0.0
8	0.0
9	0.0
10	1.0
11	23.0
12	90.0
13	518.0
14	1445.0
15	2521.0
16	4133.0
17	6479.0
18	8320.0
19	8548.0
20	7562.0
21	5850.0
22	4515.0
23	3775.0
24	3328.0
25	3335.0
26	3547.0
27	3947.0
28	4906.0
29	5618.0
30	6954.0
31	8755.0
32	11393.0
33	15257.0
34	20737.0
35	27556.0
36	32666.0
37	18787.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.07732061580866	17.669138847081438	11.633452409015902	32.620088128094004
2	19.711861025985094	17.362685186864198	39.378388670281254	23.547065116869458
3	18.526930417477004	16.765720554701772	37.694424422110096	27.012924605711124
4	13.808861063635911	19.07037260350979	32.974435926612664	34.146330406241646
5	13.728307976861842	28.278963497561065	40.40926976988776	17.583458755689342
6	26.09072116343591	33.23662211906359	18.039059241663193	22.63359747583731
7	27.106642247085063	24.282372567864073	20.799862186519665	27.811122998531197
8	22.858256864096333	31.737604729607195	19.16551811686192	26.23862028943455
9	22.44749918392514	15.562547604366909	20.516575387182183	41.47337782452577
10-11	23.708257675192517	22.98239290547663	28.06253952925335	25.246809890077504
12-13	23.257458804007044	19.05590957624064	30.09342065591683	27.593210963835485
14-15	23.596404728711164	22.053793056547317	24.14748319675383	30.20231901798769
16-17	25.37501218492062	21.928549892432656	25.941299286587068	26.755138636059655
18-19	21.57342800016322	22.002783785131687	28.602712150269994	27.821076064435108
20-21	24.697318997780755	21.487007555407455	27.368800451556307	26.446872995255482
22-23	25.977376283635213	20.461088567995827	27.094733978645753	26.466801169723208
24-25	22.09101996218836	24.57960619675654	23.889566435595533	29.439807405459568
26-27	23.833930271569116	23.70494627555878	26.807589427392664	25.65353402547944
28-29	22.735041960039354	24.162272688117444	26.545675463691303	26.5570098881519
30-31	22.303857671704588	23.992509991589607	26.818491978391418	26.885140358314384
32-33	22.463168537237237	23.79293225821808	26.730154987974093	27.01374421657059
34-35	22.677699064890906	23.76877302351941	26.613998299801644	26.939529611788043
36-37	24.406460753754605	20.86143383394729	30.604023802776993	24.12808160952111
38-39	24.044540865019506	19.714456058740986	34.11180101694992	22.129202059289586
40-41	23.1553772216177	23.121599564744287	26.187658686978597	27.53536452665941
42-43	19.995647600739908	23.50726306626528	27.88754488411737	28.609544448877443
44-45	19.934711643090317	21.53200943054044	30.586235038084876	27.947043888284366
46-47	23.426321373194416	20.692219109019522	29.6722976342682	26.209161883517858
48-49	22.95897317319768	21.085812216917617	30.89389426150352	25.061320348381187
50-51	26.43981193922935	19.67701424057089	31.162186546491544	22.720987273708214
52-53	18.685944736502062	23.095825880763414	30.445249964295673	27.772979418438847
54-55	21.9221370377255	21.810606301033246	28.915930306890875	27.35132635435038
56-57	21.942566725151767	22.434249599898447	27.991694209068363	27.631489465881426
58-59	19.08591973630818	23.50420859582745	29.32392699508752	28.085944672776858
60-61	18.789388732215563	22.753425402841884	29.191187805475206	29.265998059467353
62-63	18.988753601635842	23.615396298957876	28.555106445199595	28.840743654206687
64-65	19.54367710564793	24.07652986047355	27.62192979473404	28.75786323914448
66-67	18.179406964091406	26.009475879579252	23.64753355096119	32.16358360536815
68-69	18.797461322352653	26.143209898938135	23.677772058673476	31.381556720035736
70-71	18.42584301501842	26.975573816945307	22.925928024936244	31.672655143100027
72-73	17.907460176048275	27.518480988554366	22.03391751619141	32.540141319205944
74-75	19.071292071026843	26.67020304718121	26.127958034951227	28.13054684684072
76	19.92174571437639	25.882400946668298	27.1650276791665	27.030825659788814
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	2.0
2	3.5
3	4.5
4	6.0
5	4.5
6	2.0
7	1.0
8	1.0
9	1.0
10	1.0
11	3.0
12	5.0
13	4.5
14	5.0
15	8.0
16	12.0
17	14.0
18	17.5
19	28.5
20	48.5
21	61.0
22	86.0
23	150.0
24	232.5
25	276.0
26	369.0
27	554.0
28	787.0
29	928.0
30	1163.5
31	1631.5
32	2269.5
33	2675.0
34	3077.0
35	3930.5
36	5060.5
37	5739.0
38	6313.5
39	7676.5
40	9278.0
41	10663.0
42	11235.0
43	11807.5
44	12773.0
45	13205.0
46	13244.0
47	13134.0
48	12755.0
49	12083.0
50	11680.0
51	10967.0
52	9604.0
53	8445.0
54	7936.0
55	7334.0
56	6330.5
57	5566.5
58	5204.0
59	4861.5
60	4341.5
61	3927.5
62	3691.0
63	3540.5
64	3225.5
65	3015.5
66	2823.0
67	2676.0
68	2590.0
69	2467.5
70	2316.5
71	2202.0
72	2060.5
73	1769.5
74	1527.5
75	1435.0
76	1316.0
77	1033.5
78	753.5
79	637.0
80	572.5
81	412.5
82	261.0
83	205.0
84	160.0
85	90.0
86	51.0
87	37.0
88	31.0
89	18.0
90	10.5
91	6.5
92	3.0
93	4.0
94	3.5
95	1.0
96	0.0
97	0.5
98	1.5
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	4.5333381688940464E-4
4	0.0022666690844470234
5	0.0
6	0.0
7	0.0
8	0.009066676337788094
9	0.009066676337788094
10-11	0.01065334469690101
12-13	0.012013346147569224
14-15	0.009293343246232797
16-17	0.012920013781348032
18-19	0.011333345422235117
20-21	0.007933341795564583
22-23	0.010426677788456308
24-25	0.009520010154677499
26-27	0.008160008704009285
28-29	0.009520010154677499
30-31	0.012466679964458629
32-33	0.010200010880011606
34-35	0.01156001233067982
36-37	0.01156001233067982
38-39	0.012013346147569224
40-41	0.01269334687290333
42-43	0.009066676337788094
44-45	0.01269334687290333
46-47	0.009973343971566902
48-49	0.011333345422235117
50-51	0.009520010154677499
52-53	0.012013346147569224
54-55	0.009520010154677499
56-57	0.008613342520898688
58-59	0.012466679964458629
60-61	0.01360001450668214
62-63	0.012920013781348032
64-65	0.009293343246232797
66-67	0.01269334687290333
68-69	0.03604003844270767
70-71	0.01156001233067982
72-73	0.01065334469690101
74-75	0.01065334469690101
76	0.011333345422235117
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	220588.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	94.6331376733163
#Duplication Level	Percentage of deduplicated	Percentage of total
1	96.57416935010554	91.391166637947
2	2.382645037038257	4.509543516333704
3	0.5549958105479545	1.5756298484309503
4	0.2215263191937441	0.8385492265009838
5	0.10683871787770034	0.5055241548880508
6	0.05838333055235687	0.33149986547927535
7	0.0394534009959973	0.2613519389694337
8	0.022001942561497873	0.16656902876021001
9	0.01716475171925347	0.14619188813187659
>10	0.022821339407709765	0.2739738945585428
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0013600014506682141	0.0
2	0.0	0.0	0.0	0.0013600014506682141	0.0
3	0.0	0.0	0.0	0.0013600014506682141	0.0
4	0.0	0.0	0.0	0.0022666690844470234	0.0
5	0.0	0.0	0.0	0.004080004352004642	0.0
6	0.0	0.0	0.0	0.004080004352004642	0.0
7	0.0	0.0	0.0	0.004080004352004642	0.0
8	0.0	0.0	0.0	0.0054400058026728565	0.0
9	0.0	0.0	0.0	0.007253341070230475	0.0
10	0.0	0.0	0.0	0.008160008704009285	0.0
11	0.0	0.0	0.0	0.008160008704009285	0.0
12	0.0	0.0	0.0	0.008160008704009285	0.0
13	0.0	0.0	0.0	0.008613342520898688	0.0
14	0.0	0.0	0.0	0.009066676337788094	0.0
15	0.0	0.0	0.0	0.009066676337788094	0.0
16	0.0	0.0	0.0	0.010426677788456308	0.0
17	0.0	0.0	0.0	0.011333345422235117	0.0
18	0.0	0.0	0.0	0.013146680689792736	0.0
19	0.0	0.0	0.0	0.013600014506682141	0.0
20	0.0	0.0	0.0	0.017680018858686784	0.0
21	0.0	0.0	0.0	0.018133352675576187	0.0
22	0.0	0.0	0.0	0.021760023210691426	0.0
23	0.0	0.0	0.0	0.026293361379585472	0.0
24	0.0	0.0	0.0	0.030373365731590114	0.0
25	0.0	0.0	0.0	0.03264003481603714	0.0
26	0.0	0.0	0.0	0.03672003916804178	0.0
27	0.0	0.0	0.0	0.0766134150543094	0.0
28	0.0	0.0	0.0	0.11197345277168295	0.0
29	0.0	0.0	0.0	0.13872014796815782	0.0
30	0.0	0.0	0.0	0.19085353691043938	0.0
31	0.0	0.0	0.0	0.213973561571799	0.0
32	0.0	0.0	0.0	0.2611202785282971	0.0
33	0.0	0.0	0.0	0.32413367907592433	0.0
34	0.0	0.0	0.0	0.3835204090884364	0.0
35	0.0	0.0	0.0	0.518613886521479	0.0
36	0.0	0.0	0.0	0.6201606615047056	0.0
37	0.0	0.0	0.0	0.9637876947068743	0.0
38	0.0	0.0	0.0	1.302881389740149	0.0
39	0.0	0.0	0.0	1.6963751428001523	0.0
40	0.0	0.0	0.0	2.2231490380256407	0.0
41	0.0	0.0	0.0	2.849656372966798	0.0
42	0.0	0.0	0.0	3.4856837180626328	0.0
43	0.0	0.0	0.0	4.177471122635864	0.0
44	0.0	0.0	0.0	4.855205178885524	0.0
45	0.0	0.0	0.0	5.315792336845159	0.0
46	0.0	0.0	0.0	5.90149962826627	0.0
47	0.0	0.0	0.0	6.583767022684825	0.0
48	0.0	0.0	0.0	7.045714182095128	0.0
49	0.0	0.0	0.0	7.465047962717827	0.0
50	0.0	0.0	0.0	8.17134204943152	0.0
51	0.0	0.0	0.0	8.84862277186429	0.0
52	0.0	0.0	0.0	9.430250058933396	0.0
53	0.0	0.0	0.0	10.168730846646236	0.0
54	0.0	0.0	0.0	10.897691624204398	0.0
55	0.0	0.0	0.0	11.302065388869748	0.0
56	0.0	0.0	0.0	11.762652546829383	0.0
57	0.0	0.0	0.0	12.19150633760676	0.0
58	0.0	0.0	0.0	12.581826753948537	0.0
59	0.0	0.0	0.0	13.024280559232597	0.0
60	0.0	0.0	0.0	13.284947503944004	0.0
61	0.0	0.0	0.0	13.581881154006565	0.0
62	0.0	0.0	0.0	13.804468058099262	0.0
63	0.0	0.0	0.0	14.078735017317351	0.0
64	0.0	0.0	0.0	14.368868660126571	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCGGAC	50	9.767973E-10	55.99619	10
TAGATCT	250	0.0	54.596287	39
AGATCTC	255	0.0	53.525772	40
CTAGAGT	20	0.0069464953	52.496426	9
AGTATCG	20	0.0069464953	52.496426	35
TCTCGGT	255	0.0	49.408405	43
TCGGTGG	250	0.0	47.596764	45
CGCCGTA	200	0.0	47.246784	53
GCCGTAT	230	0.0	45.64907	54
CCTACGA	185	0.0	45.402317	21
CGGTGTT	85	1.8189894E-12	45.291035	46
GTATCAT	170	0.0	45.291035	57
GTAGATC	195	0.0	44.868744	38
CGGTGGT	290	0.0	44.652138	46
CGTATCA	190	0.0	44.20752	56
CTCGGTG	280	0.0	43.747025	44
CTCGTTG	80	2.910383E-11	43.74702	44
GGTGGTC	260	0.0	43.07399	47
CCGTATC	245	0.0	42.854225	55
GATCTCT	115	0.0	42.6058	41
>>END_MODULE