FastQCFastQC Report
Wed 25 May 2016
SRR1779743_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779743_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences220588
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT3480915.780096832103288TruSeq Adapter, Index 5 (95% over 23bp)
TCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCG38071.7258418408979637TruSeq Adapter, Index 5 (95% over 22bp)
CTCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC24831.1256278673363918RNA PCR Primer, Index 5 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTCTT15560.7053874190799136TruSeq Adapter, Index 5 (96% over 26bp)
TCTTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC10070.4565071536076305RNA PCR Primer, Index 5 (95% over 21bp)
CTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTC9630.4365604656644967TruSeq Adapter, Index 5 (95% over 24bp)
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGACCTCGTATGCCGT6180.2801602988376521No Hit
CTTTACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTC6070.27517362685186864RNA PCR Primer, Index 5 (95% over 24bp)
CTTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCG3590.16274684026329628TruSeq Adapter, Index 5 (95% over 22bp)
CTTAATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCG3280.14869349193972475TruSeq Adapter, Index 5 (95% over 22bp)
TTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTC3110.14098681705260485TruSeq Adapter, Index 5 (95% over 24bp)
TCTTAATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC3010.13645347888371082No Hit
TATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTCT2780.1260268010952545RNA PCR Primer, Index 5 (96% over 25bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCGA150.00222251570.016
GCAGCAT252.3867877E-456.00000411
TCTTTAT1850.054.8648641
CTATACA1850.052.9729731
AGCGTTA200.00694462752.510
CAGGTAA200.00694462752.59
GTCTAAC200.00694462752.514
ATCGTAT407.456547E-752.539
ATATCGT407.456547E-752.537
TGTCTAA200.00694462752.513
GTATCGG200.00694462752.539
CATGTAT200.00694462752.557
GTCCATG200.00694462752.51
TTGCAGG200.00694462752.520
TCTATAG200.00694462752.51
TCTCGTA51800.051.08108538
CTTGAAA53500.051.02803857
TTGAAAA53550.050.98039258
CCGTCTT53650.050.62441647
CGTCTTC53600.050.60634248
Produced by FastQC (version 0.11.5)