##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779743_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	220588
Sequences flagged as poor quality	0
Sequence length	76
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.69749487732787	34.0	34.0	34.0	34.0	34.0
2	33.687226866375326	34.0	34.0	34.0	34.0	34.0
3	33.71645783088835	34.0	34.0	34.0	34.0	34.0
4	33.75376720401835	34.0	34.0	34.0	34.0	34.0
5	33.757022140823615	34.0	34.0	34.0	34.0	34.0
6	37.44996101329175	38.0	38.0	38.0	37.0	38.0
7	37.43392659618837	38.0	38.0	38.0	37.0	38.0
8	37.338676627921735	38.0	38.0	38.0	37.0	38.0
9	37.33788329374218	38.0	38.0	38.0	37.0	38.0
10-11	37.27109588916895	38.0	38.0	38.0	36.0	38.0
12-13	37.241078390483615	38.0	38.0	38.0	36.0	38.0
14-15	37.25711734092516	38.0	38.0	38.0	36.0	38.0
16-17	37.21844116633724	38.0	38.0	38.0	36.0	38.0
18-19	37.211389558815526	38.0	38.0	38.0	36.0	38.0
20-21	37.238596387836154	38.0	38.0	38.0	36.0	38.0
22-23	37.19936941266071	38.0	38.0	38.0	36.0	38.0
24-25	37.15491549857653	38.0	38.0	38.0	36.0	38.0
26-27	37.088060093930764	38.0	38.0	38.0	36.0	38.0
28-29	37.071753676537256	38.0	38.0	38.0	36.0	38.0
30-31	36.911967106098245	38.0	38.0	38.0	35.0	38.0
32-33	36.85343037699241	38.0	37.5	38.0	35.0	38.0
34-35	36.79240257856275	38.0	37.0	38.0	34.5	38.0
36-37	36.90362576386748	38.0	37.0	38.0	35.0	38.0
38-39	36.09643770286688	38.0	37.0	38.0	34.0	38.0
40-41	36.73114584655557	38.0	37.0	38.0	34.5	38.0
42-43	37.02176908988703	38.0	37.5	38.0	36.0	38.0
44-45	37.043631113206516	38.0	38.0	38.0	36.0	38.0
46-47	37.06083513155747	38.0	38.0	38.0	36.0	38.0
48-49	37.03577710482891	38.0	38.0	38.0	36.0	38.0
50-51	36.96743023192558	38.0	38.0	38.0	35.0	38.0
52-53	36.96765009882677	38.0	38.0	38.0	35.5	38.0
54-55	36.961500625600664	38.0	38.0	38.0	36.0	38.0
56-57	36.97232170380982	38.0	38.0	38.0	36.0	38.0
58-59	36.89202721816237	38.0	38.0	38.0	35.0	38.0
60-61	36.938117667325514	38.0	38.0	38.0	35.5	38.0
62-63	36.98165811376865	38.0	38.0	38.0	36.0	38.0
64-65	37.00411853772644	38.0	38.0	38.0	36.0	38.0
66-67	36.92834832357154	38.0	38.0	38.0	35.0	38.0
68-69	36.729359711317024	38.0	38.0	38.0	34.5	38.0
70-71	35.284233503182406	38.0	36.5	38.0	28.0	38.0
72-73	32.882366674524455	38.0	32.5	38.0	16.0	38.0
74-75	32.555009792010445	38.0	31.5	38.0	11.0	38.0
76	32.23646798556585	38.0	30.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	2.0
21	4.0
22	26.0
23	31.0
24	47.0
25	113.0
26	211.0
27	339.0
28	606.0
29	1070.0
30	1797.0
31	2950.0
32	4562.0
33	7350.0
34	13257.0
35	30079.0
36	36957.0
37	121186.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.62603586777159	15.251509601610241	11.164705242352259	39.95774928826591
2	17.648738825321413	15.16129617204925	44.01327361415852	23.176691388470815
3	16.573884345476635	17.527245362395053	41.388470814368866	24.510399477759442
4	12.25270640288683	29.33160462037826	31.23651331894754	27.17917565778737
5	12.186519665620976	29.92320525141893	44.37548733385316	13.514787749106933
6	25.470107168114314	44.84695450341813	15.608283315502202	14.074655012965348
7	23.823598745171996	25.3508803742724	17.631058806462725	33.194462074092876
8	20.919995647995357	44.282553901390834	15.19982954648485	19.597620904128966
9	19.724554372857998	15.785536837905962	15.566576604348379	48.92333218488766
10-11	21.46331622753731	28.848350771574154	28.256750140533484	21.43158286035505
12-13	21.08024915226576	17.802192322338477	29.788111773985893	31.32944675140987
14-15	19.755834406223364	17.699738879721473	19.17760712278093	43.36681959127423
16-17	29.596124902533226	28.356030246432262	18.47085970225035	23.576985148784157
18-19	29.8028451230348	21.739396522022957	19.037980307178994	29.419778047763252
20-21	20.345168368179593	19.921527916296444	18.82944675140987	40.90385696411409
22-23	20.270594955301284	29.0818176872722	19.115273722958637	31.53231363446788
24-25	29.427711389558812	29.680898326291548	18.73356664913776	22.157823635011876
26-27	29.29737791720311	31.017779752298402	19.132500408000435	20.55234192249805
28-29	19.794367780658966	31.15219322900611	19.79164777775763	29.261791212577293
30-31	19.207300487787187	31.195713275427494	19.36415398843092	30.232832248354395
32-33	19.453007416541244	40.332203021016554	19.79550111520119	20.41928844724101
34-35	29.141884418010044	22.605490779190166	29.156391100150508	19.096233702649283
36-37	29.978511977079442	27.825176346854768	22.333717155964965	19.86259452010082
38-39	19.817261138411883	21.5342629698805	29.935671931383396	28.712803960324223
40-41	18.95978022376557	18.88452681016193	31.438246867463327	30.71744609860917
42-43	28.514697082343552	18.47992637858814	30.965646363356118	22.039730175712187
44-45	19.113233720782635	27.98610985185051	32.30184778863764	20.598808638729217
46-47	27.491295990715724	19.20118048125918	24.03689230601846	29.27063122200664
48-49	27.05110885451611	18.218126099334505	22.60050410720438	32.130260938945
50-51	18.869340127296137	18.51619308393929	32.36622119063593	30.248245598128637
52-53	16.59519103487044	18.698659945237274	41.99299145919089	22.713157560701397
54-55	16.30165738843455	18.902886829745952	33.94223620505195	30.85321957676755
56-57	25.475093840100094	18.957286887772682	24.628492937059132	30.93912633506809
58-59	17.397138556947795	19.569287540573377	39.75125573467279	23.282318167806046
60-61	25.665947377010532	29.11853772644024	23.680118592126497	21.535396304422726
62-63	16.567537672040185	41.30959073023011	20.070901408961504	22.051970188768202
64-65	15.93695033274702	42.590938763668014	19.299780586432625	22.17233031715234
66-67	16.053683790596043	42.52021868823327	18.722233303715523	22.703864217455166
68-69	16.125990534389903	42.717418898580156	18.573313144867353	22.583277422162585
70-71	16.48253758137342	41.08632382541208	18.67871325729414	23.752425335920357
72-73	17.083431555660326	36.61939906069233	19.939434602063574	26.35773478158377
74-75	17.37809853663844	35.19547754184271	20.473008504542406	26.953415416976444
76	17.285165104176112	35.807931528460294	20.345621701996482	26.561281665367108
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.5
11	0.5
12	0.0
13	0.5
14	0.5
15	6.5
16	17.0
17	21.0
18	27.0
19	41.0
20	60.0
21	71.0
22	112.0
23	189.0
24	289.0
25	353.0
26	405.0
27	497.5
28	737.0
29	936.0
30	1049.0
31	1289.5
32	1863.5
33	2310.0
34	2533.5
35	3212.5
36	3991.0
37	4314.0
38	5107.0
39	13834.0
40	22711.0
41	21593.0
42	19532.0
43	17124.5
44	13423.0
45	11310.5
46	10492.0
47	10385.0
48	9764.5
49	8932.0
50	8613.0
51	8050.5
52	6686.0
53	5446.0
54	5008.0
55	4844.5
56	4249.5
57	3634.5
58	3451.0
59	3150.5
60	2782.5
61	2684.0
62	2653.0
63	2614.5
64	2459.5
65	2345.0
66	2342.5
67	2338.0
68	2265.0
69	2214.0
70	2103.0
71	1970.0
72	2002.0
73	1912.5
74	1683.5
75	1576.0
76	1562.5
77	1371.5
78	1072.5
79	951.0
80	823.0
81	602.0
82	464.0
83	419.0
84	329.0
85	180.0
86	96.5
87	72.0
88	63.5
89	34.0
90	9.5
91	3.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	220588.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.89997642664152
#Duplication Level	Percentage of deduplicated	Percentage of total
1	42.42330690237879	11.836092625165467
2	23.3199012088912	13.012493879993473
3	14.171974522292993	11.861932652728163
4	7.402833744962953	8.261555478992511
5	3.842779149876511	5.36067238471721
6	2.209801117899389	3.699203945817542
7	1.2852593266606005	2.510109344116634
8	0.8952944235018849	1.9982954648484959
9	0.7393084622383985	1.856401980162112
>10	3.6282984531392177	16.7679112191053
>50	0.050370466658000775	0.8685875931600994
>100	0.01787339139477447	1.1813879268137886
>500	0.004874561289483946	0.9918943913540176
>1k	0.006499415052645263	4.013364280921899
>5k	0.0	0.0
>10k+	0.0016248537631613157	15.780096832103288
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT	34809	15.780096832103288	TruSeq Adapter, Index 5 (95% over 23bp)
TCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCG	3807	1.7258418408979637	TruSeq Adapter, Index 5 (95% over 22bp)
CTCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC	2483	1.1256278673363918	RNA PCR Primer, Index 5 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTCTT	1556	0.7053874190799136	TruSeq Adapter, Index 5 (96% over 26bp)
TCTTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC	1007	0.4565071536076305	RNA PCR Primer, Index 5 (95% over 21bp)
CTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTC	963	0.4365604656644967	TruSeq Adapter, Index 5 (95% over 24bp)
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGACCTCGTATGCCGT	618	0.2801602988376521	No Hit
CTTTACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTC	607	0.27517362685186864	RNA PCR Primer, Index 5 (95% over 24bp)
CTTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCG	359	0.16274684026329628	TruSeq Adapter, Index 5 (95% over 22bp)
CTTAATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCG	328	0.14869349193972475	TruSeq Adapter, Index 5 (95% over 22bp)
TTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTC	311	0.14098681705260485	TruSeq Adapter, Index 5 (95% over 24bp)
TCTTAATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC	301	0.13645347888371082	No Hit
TATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTCT	278	0.1260268010952545	RNA PCR Primer, Index 5 (96% over 25bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0018133352675576188	0.0
2	0.0	0.0	0.0	0.0018133352675576188	0.0
3	0.0	0.0	0.0	0.0018133352675576188	0.0
4	0.0	0.0	0.0	0.0018133352675576188	0.0
5	0.0	0.0	0.0	0.0054400058026728565	0.0
6	0.0	0.0	0.0	0.0054400058026728565	0.0
7	0.0	0.0	0.0	0.0054400058026728565	0.0
8	0.0	0.0	0.0	0.008160008704009285	0.0
9	0.0	0.0	0.0	0.01269334687290333	0.0
10	0.0	0.0	0.0	0.015413349774239759	0.0
11	0.0	0.0	0.0	0.015866683591129162	0.0
12	0.0	0.0	0.0	0.018133352675576187	0.0
13	0.0	0.0	0.0	0.018133352675576187	0.0
14	0.0	0.0	0.0	0.01858668649246559	0.0
15	0.0	0.0	0.0	0.020400021760023212	0.0
16	0.0	0.0	0.0	0.021760023210691426	0.0
17	0.0	0.0	0.0	0.023573358478249044	0.0
18	0.0	0.0	0.0	0.02538669374580666	0.0
19	0.0	0.0	0.0	0.026293361379585472	0.0
20	0.0	0.0	0.0	0.031733367182258325	0.0
21	0.0	0.0	0.0	0.03309336863292654	0.0
22	0.0	0.0	0.0	0.034906703900484164	0.0
23	0.0	0.0	0.0	0.04261337878760404	0.0
24	0.0	0.0	0.0	0.04578671550582987	0.0
25	0.0	0.0	0.0	0.04941338604094511	0.0
26	0.0	0.0	0.0	0.05349339039294975	0.0
27	0.0	0.0	0.0	0.10336011025078427	0.0
28	0.0	0.0	0.0	0.14960015957350353	0.0
29	0.0	0.0	0.0	0.20037354706511687	0.0
30	0.0	0.0	0.0	0.26701361814785934	0.0
31	0.0	0.0	0.0	0.3468003699203946	0.0
32	0.0	0.0	0.0	0.4274937893267086	0.0
33	0.0	0.0	0.0	0.5517072551544056	0.0
34	0.0	0.0	0.0	0.7076540881643607	0.0
35	0.0	0.0	0.0	0.908480969046367	0.0
36	0.0	0.0	0.0	1.2276279761365079	0.0
37	0.0	0.0	0.0	1.739895189221535	0.0
38	0.0	0.0	0.0	2.3092824632346276	0.0
39	0.0	0.0	0.0	2.906776433894863	0.0
40	0.0	0.0	0.0	3.650697227410376	0.0
41	0.0	0.0	0.0	4.4861914519375485	0.0
42	0.0	0.0	0.0	5.3751790668576716	0.0
43	0.0	0.0	0.0	6.323100077973416	0.0
44	0.0	0.0	0.0	7.187607666781512	0.0
45	0.0	0.0	0.0	8.097448637278546	0.0
46	0.0	0.0	0.0	9.01590295029648	0.0
47	0.0	0.0	0.0	9.938890601483308	0.0
48	0.0	0.0	0.0	10.742651458828222	0.0
49	0.0	0.0	0.0	11.600359040382976	0.0
50	0.0	0.0	0.0	12.45715995430395	0.0
51	0.0	0.0	0.0	13.247320797142184	0.0
52	0.0	0.0	0.0	14.063321667543113	0.0
53	0.0	0.0	0.0	14.868895860155584	0.0
54	0.0	0.0	0.0	15.69351007307741	0.0
55	0.0	0.0	0.0	16.35673744718661	0.0
56	0.0	0.0	0.0	17.03628483870383	0.0
57	0.0	0.0	0.0	17.771138955881554	0.0
58	0.0	0.0	0.0	18.444793007779207	0.0
59	0.0	0.0	0.0	19.08490035722705	0.0
60	0.0	0.0	0.0	19.67695432208461	0.0
61	0.0	0.0	0.0	20.318421672983117	0.0
62	0.0	0.0	0.0	20.9354089977696	0.0
63	0.0	0.0	0.0	21.498449598346237	0.0
64	0.0	0.0	0.0	22.116796924583387	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCGA	15	0.002222515	70.0	16
GCAGCAT	25	2.3867877E-4	56.000004	11
TCTTTAT	185	0.0	54.864864	1
CTATACA	185	0.0	52.972973	1
AGCGTTA	20	0.006944627	52.5	10
CAGGTAA	20	0.006944627	52.5	9
GTCTAAC	20	0.006944627	52.5	14
ATCGTAT	40	7.456547E-7	52.5	39
ATATCGT	40	7.456547E-7	52.5	37
TGTCTAA	20	0.006944627	52.5	13
GTATCGG	20	0.006944627	52.5	39
CATGTAT	20	0.006944627	52.5	57
GTCCATG	20	0.006944627	52.5	1
TTGCAGG	20	0.006944627	52.5	20
TCTATAG	20	0.006944627	52.5	1
TCTCGTA	5180	0.0	51.081085	38
CTTGAAA	5350	0.0	51.028038	57
TTGAAAA	5355	0.0	50.980392	58
CCGTCTT	5365	0.0	50.624416	47
CGTCTTC	5360	0.0	50.606342	48
>>END_MODULE