FastQCFastQC Report
Wed 25 May 2016
SRR1779742_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779742_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences117327
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGTAC252.3821658E-455.9999964
TCGTGTC252.3821658E-455.99999634
CGCCGTA653.6379788E-1253.84615353
GGCCGTT200.006936562252.514
CCCGTGT200.006936562252.533
TCTCGAC200.006936562252.537
GCCGTTA200.006936562252.515
CTTCCGA552.2573658E-950.9090918
GTGTACA305.8577186E-446.66666836
ACTGTGT305.8577186E-446.66666833
CAGTGTA451.6727827E-646.66666434
TCGGTGG802.910383E-1143.7545
CTGTGTA404.5694964E-543.7534
GCCGTAT659.893483E-943.07692354
CCTACGA503.4541372E-642.021
GTAGATC503.4541372E-642.038
TAGATCT757.4396667E-1042.039
TTCCGAC602.5732697E-740.83333619
TCTCGGT953.6379788E-1240.52631843
ATACTGG350.001251165440.053