##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779742_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	117327
Sequences flagged as poor quality	0
Sequence length	76
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.042769354027634	34.0	31.0	34.0	21.0	34.0
2	28.456263264210286	34.0	23.0	34.0	12.0	34.0
3	27.34474588116972	33.0	23.0	34.0	11.0	34.0
4	29.899059892437375	34.0	31.0	34.0	21.0	34.0
5	29.388273798869825	34.0	27.0	34.0	11.0	34.0
6	31.93673238044099	37.0	28.0	38.0	12.0	38.0
7	32.95452027240107	37.0	32.0	38.0	21.0	38.0
8	32.17577369232998	37.0	31.0	38.0	11.0	38.0
9	33.58855165477682	37.0	34.0	38.0	21.0	38.0
10-11	33.36983388307891	38.0	34.0	38.0	21.0	38.0
12-13	32.2819001593836	37.5	30.0	38.0	16.0	38.0
14-15	32.38740869535572	37.0	31.5	38.0	11.5	38.0
16-17	32.03104570985366	36.5	29.5	38.0	16.0	38.0
18-19	32.60394879269052	37.0	31.5	38.0	16.0	38.0
20-21	31.462459621400022	36.5	28.0	38.0	16.0	38.0
22-23	31.404685196075924	36.0	28.5	38.0	16.0	38.0
24-25	32.74485412564883	37.0	32.5	38.0	16.5	38.0
26-27	31.2308718368321	36.5	27.5	38.0	11.0	38.0
28-29	33.47040749358631	37.5	34.0	38.0	21.0	38.0
30-31	33.903730599094835	38.0	34.0	38.0	21.0	38.0
32-33	34.013347311360555	38.0	35.0	38.0	21.0	38.0
34-35	34.074735568113056	38.0	35.0	38.0	21.0	38.0
36-37	31.79675607490177	37.0	29.0	38.0	16.0	38.0
38-39	29.948677627485573	35.0	24.0	38.0	11.0	38.0
40-41	31.331206798094215	36.5	28.5	38.0	11.0	38.0
42-43	32.43301627076461	37.0	30.5	38.0	16.0	38.0
44-45	34.089242032950644	38.0	35.0	38.0	21.5	38.0
46-47	32.58434972342257	37.5	30.5	38.0	16.5	38.0
48-49	31.993458453723353	36.5	30.0	38.0	16.0	38.0
50-51	29.683129202996753	35.0	23.5	38.0	11.0	38.0
52-53	33.261478602538205	37.0	33.0	38.0	21.0	38.0
54-55	32.276581690488975	37.0	30.0	38.0	16.0	38.0
56-57	32.71010935249346	37.5	30.5	38.0	16.0	38.0
58-59	33.87005548594952	37.5	34.5	38.0	21.0	38.0
60-61	34.313389074978474	38.0	36.0	38.0	22.5	38.0
62-63	34.20883087439379	38.0	35.5	38.0	22.0	38.0
64-65	33.87588534608402	38.0	35.0	38.0	20.5	38.0
66-67	31.72284299436617	37.0	27.0	38.0	11.0	38.0
68-69	31.80452069856043	37.0	29.0	38.0	11.0	38.0
70-71	31.311969964287844	36.5	27.0	38.0	11.0	38.0
72-73	30.088261866407564	35.0	24.5	38.0	11.0	38.0
74-75	32.42525590869962	36.5	30.5	38.0	16.0	38.0
76	32.92110937806302	37.0	33.0	38.0	20.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	25.0
13	133.0
14	445.0
15	769.0
16	1170.0
17	1727.0
18	2201.0
19	2288.0
20	2093.0
21	1638.0
22	1482.0
23	1258.0
24	1281.0
25	1368.0
26	1562.0
27	1918.0
28	2341.0
29	2820.0
30	3691.0
31	4978.0
32	6860.0
33	9709.0
34	13682.0
35	18544.0
36	21526.0
37	11817.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.62282339103531	19.84965097547879	12.847000264218808	28.68052536926709
2	21.065057488898546	17.495546634619483	38.84272162417858	22.59667425230339
3	20.657649134470326	19.371500166202154	34.951886607515746	25.018964091811775
4	14.222642699463892	18.728851841434626	34.191618297578565	32.85688716152293
5	15.013594483793161	30.6919975794148	37.847213343902084	16.447194592889957
6	27.882755035072915	33.77653907455232	16.605725877249057	21.73498001312571
7	26.7142260519744	26.877871248732177	21.52616192351292	24.88174077578051
8	22.808901616848637	32.84495469925934	18.19018640210693	26.155957281785096
9	23.86918612084175	16.32616533278785	20.00306834743921	39.80158019893119
10-11	25.513100250583843	24.165572848303018	26.49625828887033	23.825068612242813
12-13	24.93245374040076	20.182481440759588	28.283344839636232	26.601719979203427
14-15	23.998312408908433	22.77353038942443	23.369301183870718	29.858856017796416
16-17	24.971447079078807	23.467944019228472	25.850621345652286	25.70998755604044
18-19	22.99300246320114	23.082922089544606	27.33812336461343	26.585952082640823
20-21	24.209687454720566	22.975529929172314	27.159136430659608	25.655646185447512
22-23	25.01725945434555	22.076759825104194	27.349203508143905	25.556777212406352
24-25	23.497148993837737	24.56893980072788	24.099312178782377	27.834599026652008
26-27	23.449845304149942	23.70681940218364	27.135271506132437	25.708063787533987
28-29	23.581954707782522	24.567235163261653	25.87085666555865	25.97995346339717
30-31	23.629661197528236	24.298316641806945	25.758363520136374	26.31365864052845
32-33	23.23974873643748	24.91966895940406	25.639025970151796	26.201556334006664
34-35	23.772447944633377	24.320062730658755	25.747270449257204	26.160218875450663
36-37	24.368644898446224	22.367826672462435	29.29462101647532	23.968907412616023
38-39	23.958253428452103	21.67233458624187	32.02885951230322	22.340552473002802
40-41	23.75369693250488	23.430241973288332	25.239288484321598	27.576772609885193
42-43	22.060565769175042	23.74432142644063	26.20113017464011	27.993982629744217
44-45	22.16208614422147	23.500658419027246	27.605442930625223	26.73181250612607
46-47	23.57556231728417	22.47479267346817	28.703111815694598	25.246533193553063
48-49	23.87440177623981	22.907015891550504	28.577090427141354	24.641491905068335
50-51	24.51993147357386	21.35825513308957	30.98008131120714	23.141732082129433
52-53	21.521900329847348	24.044763779863118	27.97480545824917	26.458530432040366
54-55	22.971464125598757	23.255714845814225	27.811397303240543	25.96142372534647
56-57	22.93291399251664	23.387199877266102	27.436992337654587	26.242893792562665
58-59	21.483729096704906	24.509060225354993	27.452141895232092	26.55506878270801
60-61	21.668072992576302	23.385495239799877	27.36454524533995	27.58188652228387
62-63	21.16027138059765	24.261033360039548	27.25440226377785	27.324292995584955
64-65	21.53383279211094	24.89750867234311	26.24502458939545	27.323633946150505
66-67	20.77782607583932	24.427028731664493	23.731962804810486	31.0631823876857
68-69	20.809499859343433	25.07182009598745	23.96958408279131	30.14909596187781
70-71	21.12471980021649	24.588969290956044	23.51206457166722	30.774246337160243
72-73	20.500822487577455	23.969333571982578	22.758188652228387	32.77165528821158
74-75	21.629292490219644	24.824209261295355	24.5953616814544	28.951136567030606
76	22.041814757046545	24.235683176080528	25.271250436813354	28.451251630059577
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.5
12	1.0
13	1.5
14	2.0
15	3.5
16	5.0
17	5.0
18	8.5
19	15.0
20	25.0
21	32.0
22	43.0
23	77.0
24	135.5
25	171.0
26	222.5
27	340.5
28	512.0
29	617.0
30	739.5
31	1005.0
32	1330.5
33	1513.0
34	1764.0
35	2313.5
36	2925.5
37	3239.0
38	3560.5
39	4284.5
40	5049.5
41	5742.5
42	6073.0
43	6280.5
44	6555.0
45	6662.5
46	6703.0
47	6625.0
48	6317.0
49	5844.0
50	5601.0
51	5260.0
52	4612.5
53	4032.5
54	3759.0
55	3487.5
56	3024.0
57	2601.0
58	2370.0
59	2303.5
60	2167.0
61	2029.5
62	1962.0
63	1938.0
64	1889.0
65	1858.5
66	1850.5
67	1848.0
68	1763.0
69	1681.5
70	1594.5
71	1504.0
72	1422.5
73	1252.0
74	1091.5
75	1020.0
76	902.0
77	683.0
78	516.5
79	451.0
80	374.0
81	236.5
82	152.0
83	128.0
84	103.5
85	57.5
86	26.0
87	16.0
88	10.0
89	3.5
90	5.0
91	3.5
92	0.0
93	0.0
94	0.5
95	0.5
96	0.0
97	0.5
98	0.5
99	1.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	8.52318733113435E-4
12-13	0.0
14-15	0.0
16-17	8.52318733113435E-4
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.00170463746622687
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	4.261593665567175E-4
46-47	0.0
48-49	4.261593665567175E-4
50-51	0.0
52-53	0.0
54-55	8.52318733113435E-4
56-57	0.0
58-59	8.52318733113435E-4
60-61	0.0
62-63	8.52318733113435E-4
64-65	0.0
66-67	0.0
68-69	0.0170463746622687
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	117327.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	93.9730515880415
#Duplication Level	Percentage of deduplicated	Percentage of total
1	95.81862651958903	90.04368733020621
2	3.0059191464232193	5.649507900326217
3	0.6696007623221731	1.8877328094328047
4	0.24394553534465108	0.916972255104612
5	0.12895562331782257	0.6059176721306894
6	0.0649767929500757	0.36636405095537733
7	0.02898960534468345	0.1906969175001031
8	0.017993545417946538	0.13527266974499663
9	0.009996413957710872	0.08454541720890435
>10	0.010996055332686156	0.11930297739011254
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0034092749324537404	0.0
2	0.0	0.0	0.0	0.0034092749324537404	0.0
3	0.0	0.0	0.0	0.0034092749324537404	0.0
4	0.0	0.0	0.0	0.004261593665567175	0.0
5	0.0	0.0	0.0	0.004261593665567175	0.0
6	0.0	0.0	0.0	0.004261593665567175	0.0
7	8.523187331134351E-4	0.0	0.0	0.006818549864907481	0.0
8	8.523187331134351E-4	0.0	0.0	0.006818549864907481	0.0
9	8.523187331134351E-4	0.0	0.0	0.007670868598020916	0.0
10	8.523187331134351E-4	0.0	0.0	0.007670868598020916	0.0
11	8.523187331134351E-4	0.0	0.0	0.007670868598020916	0.0
12	8.523187331134351E-4	0.0	0.0	0.007670868598020916	0.0
13	8.523187331134351E-4	0.0	0.0	0.007670868598020916	0.0
14	8.523187331134351E-4	0.0	0.0	0.009375506064247786	0.0
15	8.523187331134351E-4	0.0	0.0	0.009375506064247786	0.0
16	8.523187331134351E-4	0.0	0.0	0.009375506064247786	0.0
17	8.523187331134351E-4	0.0	0.0	0.009375506064247786	0.0
18	8.523187331134351E-4	0.0	0.0	0.011080143530474656	0.0
19	8.523187331134351E-4	0.0	0.0	0.011080143530474656	0.0
20	8.523187331134351E-4	0.0	0.0	0.011932462263588092	0.0
21	8.523187331134351E-4	0.0	0.0	0.011932462263588092	0.0
22	8.523187331134351E-4	0.0	0.0	0.017898693395382137	0.0
23	8.523187331134351E-4	8.523187331134351E-4	0.0	0.01960333086160901	0.0
24	8.523187331134351E-4	8.523187331134351E-4	0.0	0.028978836925856795	0.0
25	8.523187331134351E-4	8.523187331134351E-4	0.0	0.029831155658970227	0.0
26	8.523187331134351E-4	8.523187331134351E-4	0.0	0.0340927493245374	0.0
27	8.523187331134351E-4	8.523187331134351E-4	0.0	0.04517289285501206	0.0
28	8.523187331134351E-4	8.523187331134351E-4	0.0	0.0681854986490748	0.0
29	8.523187331134351E-4	8.523187331134351E-4	0.0	0.08778882951068381	0.0
30	8.523187331134351E-4	8.523187331134351E-4	0.0	0.11335839150408687	0.0
31	8.523187331134351E-4	8.523187331134351E-4	0.0	0.12188157883522122	0.0
32	8.523187331134351E-4	8.523187331134351E-4	0.0	0.1483034595617377	0.0
33	8.523187331134351E-4	8.523187331134351E-4	0.0	0.17387302155514076	0.0
34	8.523187331134351E-4	8.523187331134351E-4	0.0	0.20711345214656474	0.0
35	8.523187331134351E-4	8.523187331134351E-4	0.0	0.2889360505254545	0.0
36	8.523187331134351E-4	8.523187331134351E-4	0.0	0.3426321307116009	0.0
37	8.523187331134351E-4	8.523187331134351E-4	0.0	0.5480409453919388	0.0
38	8.523187331134351E-4	8.523187331134351E-4	0.0	0.7372557041431214	0.0
39	8.523187331134351E-4	8.523187331134351E-4	0.0	0.9742003119486563	0.0
40	8.523187331134351E-4	8.523187331134351E-4	0.0	1.2520562189436362	0.0
41	8.523187331134351E-4	8.523187331134351E-4	0.0	1.5989499433208043	0.0
42	8.523187331134351E-4	8.523187331134351E-4	0.0	1.9074893247078677	0.0
43	8.523187331134351E-4	8.523187331134351E-4	0.0	2.2381889931558807	0.0
44	8.523187331134351E-4	8.523187331134351E-4	0.0	2.5748548927356874	0.0
45	8.523187331134351E-4	8.523187331134351E-4	0.0	2.859529349595575	0.0
46	8.523187331134351E-4	8.523187331134351E-4	0.0	3.189376699310474	0.0
47	8.523187331134351E-4	8.523187331134351E-4	0.0	3.5899665038737885	0.0
48	8.523187331134351E-4	8.523187331134351E-4	0.0	3.8771979169330164	0.0
49	8.523187331134351E-4	8.523187331134351E-4	0.0	4.157610780127336	0.0
50	8.523187331134351E-4	8.523187331134351E-4	0.0	4.572690003153579	0.0
51	8.523187331134351E-4	8.523187331134351E-4	0.0	4.970722851517554	0.0
52	8.523187331134351E-4	8.523187331134351E-4	0.0	5.343186137888124	0.0
53	8.523187331134351E-4	8.523187331134351E-4	0.0	5.815370716032968	0.0
54	8.523187331134351E-4	8.523187331134351E-4	0.0	6.280736744312903	0.0
55	8.523187331134351E-4	8.523187331134351E-4	0.0	6.577343663436379	0.0
56	8.523187331134351E-4	8.523187331134351E-4	0.0	6.904634056951938	0.0
57	8.523187331134351E-4	8.523187331134351E-4	0.0	7.207207207207207	0.0
58	8.523187331134351E-4	8.523187331134351E-4	0.0	7.462902827141238	0.0
59	8.523187331134351E-4	8.523187331134351E-4	0.0	7.7936024955892504	0.0
60	8.523187331134351E-4	8.523187331134351E-4	0.0	8.021171597330538	0.0
61	8.523187331134351E-4	8.523187331134351E-4	0.0	8.253002292737392	0.0
62	8.523187331134351E-4	8.523187331134351E-4	0.0	8.435398501623666	0.0
63	8.523187331134351E-4	8.523187331134351E-4	0.0	8.662115284631842	0.0
64	8.523187331134351E-4	8.523187331134351E-4	0.0	8.894798298771809	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGGGTAC	25	2.3821658E-4	55.999996	4
TCGTGTC	25	2.3821658E-4	55.999996	34
CGCCGTA	65	3.6379788E-12	53.846153	53
GGCCGTT	20	0.0069365622	52.5	14
CCCGTGT	20	0.0069365622	52.5	33
TCTCGAC	20	0.0069365622	52.5	37
GCCGTTA	20	0.0069365622	52.5	15
CTTCCGA	55	2.2573658E-9	50.90909	18
GTGTACA	30	5.8577186E-4	46.666668	36
ACTGTGT	30	5.8577186E-4	46.666668	33
CAGTGTA	45	1.6727827E-6	46.666664	34
TCGGTGG	80	2.910383E-11	43.75	45
CTGTGTA	40	4.5694964E-5	43.75	34
GCCGTAT	65	9.893483E-9	43.076923	54
CCTACGA	50	3.4541372E-6	42.0	21
GTAGATC	50	3.4541372E-6	42.0	38
TAGATCT	75	7.4396667E-10	42.0	39
TTCCGAC	60	2.5732697E-7	40.833336	19
TCTCGGT	95	3.6379788E-12	40.526318	43
ATACTGG	35	0.0012511654	40.0	53
>>END_MODULE