FastQCFastQC Report
Wed 25 May 2016
SRR1779742_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779742_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences117327
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGT74216.325057318434801No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG10040.8557280080458888No Hit
ATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTCTT8190.6980490424199033RNA PCR Primer, Index 48 (95% over 23bp)
CTCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC4760.40570371696199514No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC4110.35030299930962183No Hit
CTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTC3820.3255857560493322No Hit
CTTTACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTC3020.2574002574002574RNA PCR Primer, Index 48 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAACCTCGTATGCCGT1490.12699549123390183No Hit
CTTAATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG1400.11932462263588091No Hit
TATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTCT1360.11591534770342717RNA PCR Primer, Index 48 (95% over 22bp)
TCTTAATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC1320.11250607277097342No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAGGAC150.002219913370.0000145
AATATCG150.002219913370.0000136
ATATCGT150.002219913370.0000137
GTTCTGA252.3821658E-455.99999611
ATCGTAT252.3821658E-455.99999639
TCTTTAT452.6757334E-854.4444431
CTTGTTG200.006936562252.513
CTTAACT200.006936562252.554
ATGGGAC200.006936562252.561
TCGTTTA200.006936562252.532
CAACGAC200.006936562252.531
GGTCATC200.006936562252.511
GCTTAAC200.006936562252.513
ATCCATG200.006936562252.515
GGTGAAT200.006936562252.532
AAGGGAT200.006936562252.530
ATTATAG200.006936562252.569
CGGTAAA200.006936562252.558
GCATGCT200.006936562252.523
CGTTTAT200.006936562252.533
Produced by FastQC (version 0.11.5)