##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779742_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	117327
Sequences flagged as poor quality	0
Sequence length	76
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.47878152513914	34.0	34.0	34.0	34.0	34.0
2	33.49941616166782	34.0	34.0	34.0	34.0	34.0
3	33.52057923581103	34.0	34.0	34.0	34.0	34.0
4	33.572886036462194	34.0	34.0	34.0	34.0	34.0
5	33.562223529110945	34.0	34.0	34.0	34.0	34.0
6	37.05246021802313	38.0	37.0	38.0	35.0	38.0
7	37.06963444049537	38.0	37.0	38.0	36.0	38.0
8	36.86314318102398	38.0	37.0	38.0	35.0	38.0
9	36.88580633613746	38.0	37.0	38.0	35.0	38.0
10-11	36.78187458982161	38.0	37.0	38.0	34.0	38.0
12-13	36.755273722161135	38.0	37.0	38.0	34.0	38.0
14-15	36.792792792792795	38.0	37.0	38.0	34.0	38.0
16-17	36.74152155940236	38.0	37.0	38.0	34.0	38.0
18-19	36.75059875391001	38.0	37.0	38.0	34.0	38.0
20-21	36.79058102568037	38.0	37.0	38.0	34.0	38.0
22-23	36.67154619141374	38.0	37.0	38.0	34.0	38.0
24-25	36.655207241299955	38.0	37.0	38.0	34.0	38.0
26-27	36.53394359354624	38.0	37.0	38.0	34.0	38.0
28-29	36.47690216233263	38.0	37.0	38.0	34.0	38.0
30-31	36.44312477093934	38.0	37.0	38.0	34.0	38.0
32-33	36.38664587008958	38.0	37.0	38.0	34.0	38.0
34-35	36.31588210727284	38.0	37.0	38.0	34.0	38.0
36-37	36.353337253999506	38.0	37.0	38.0	34.0	38.0
38-39	36.06462280634466	38.0	37.0	38.0	34.0	38.0
40-41	36.3227177035124	38.0	37.0	38.0	34.0	38.0
42-43	36.4563357113026	38.0	37.0	38.0	34.0	38.0
44-45	36.45209968719902	38.0	37.0	38.0	34.0	38.0
46-47	36.47607967475517	38.0	37.0	38.0	34.0	38.0
48-49	36.457614189402264	38.0	37.0	38.0	34.0	38.0
50-51	36.40286123398706	38.0	37.0	38.0	34.0	38.0
52-53	36.35603484278981	38.0	37.0	38.0	34.0	38.0
54-55	36.36803975214571	38.0	37.0	38.0	34.0	38.0
56-57	36.37105269886727	38.0	37.0	38.0	34.0	38.0
58-59	36.30579065347277	38.0	37.0	38.0	34.0	38.0
60-61	36.37458982160969	38.0	37.0	38.0	34.0	38.0
62-63	36.36198402754694	38.0	37.0	38.0	34.0	38.0
64-65	36.38953096900117	38.0	37.0	38.0	34.0	38.0
66-67	36.33144118574582	38.0	37.0	38.0	34.0	38.0
68-69	36.153860577701636	38.0	37.0	38.0	34.0	38.0
70-71	35.515422707475686	38.0	36.5	38.0	29.5	38.0
72-73	34.56185277046204	38.0	36.0	38.0	23.0	38.0
74-75	34.46673399984658	38.0	36.0	38.0	23.0	38.0
76	33.86513760685946	38.0	34.0	38.0	22.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	2.0
21	3.0
22	4.0
23	31.0
24	51.0
25	69.0
26	162.0
27	362.0
28	592.0
29	973.0
30	1582.0
31	2356.0
32	3678.0
33	5665.0
34	8821.0
35	14812.0
36	20285.0
37	57879.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.35031152249695	19.11495222753501	12.018546455632547	31.516189794335492
2	19.088530346808493	18.76720618442473	38.61174324750484	23.532520221261944
3	18.354683917597825	21.411098894542604	34.03393933195258	26.200277855906997
4	14.047065040442524	22.791002923453256	34.37742378139729	28.78450825470693
5	14.047917359175639	33.276227978214735	37.97165187893665	14.704202783672981
6	28.029353857168427	40.00784133234465	16.809430054463167	15.153374756023762
7	26.376707833661477	28.15975862333478	20.43008003272904	25.0334535102747
8	23.148124472627785	38.59469687284257	18.11773930979229	20.139439344737358
9	23.08334824891116	16.330426926453416	18.83794863927314	41.74827618536228
10-11	24.65885942707135	26.531829843088122	26.53481295865402	22.274497771186514
12-13	24.243354044678547	20.07977703341942	26.81778277804768	28.859086143854356
14-15	22.879644071697054	20.972154746989183	21.011361408712403	35.13683977260136
16-17	27.175330486588766	26.799031765919185	21.057812779667085	24.967824967824967
18-19	26.637091206627627	23.757958526170448	22.35163261653328	27.253317650668645
20-21	22.80719697938241	23.111474767103907	21.783136021546618	32.298192231967064
22-23	23.342026984411092	26.87616661126595	22.006869688988896	27.774936715334064
24-25	27.015094564763437	27.151039402695034	21.849616882729467	23.984249149812065
26-27	26.472593691136737	27.64879354283328	23.061187961850212	22.81742480417977
28-29	22.47436651410161	23.407229367494267	27.840991417150356	26.27741270125376
30-31	22.782053576755565	21.818933408337383	32.32035251902802	23.07866049587904
32-33	25.492427148056287	23.241879533270264	24.349467726951172	26.91622559172228
34-35	23.506098340535427	23.054369411985306	27.106292669206578	26.333239578272693
36-37	22.677644531949166	29.852889786664623	23.59516564814578	23.87430003324043
38-39	22.012835920120686	24.39378830107307	27.11609433463738	26.47728144416886
40-41	22.045224031979	22.04863330691145	28.43889300842943	27.467249652680113
42-43	26.491344703265234	22.101477068364485	27.838434460951017	23.568743767419264
44-45	21.983430923828276	26.23607524269776	28.461905614223493	23.318588219250472
46-47	26.040468093448226	22.372088266128003	25.056039956702207	26.531403683721567
48-49	25.373954844153516	21.630144808952757	24.44705822189266	28.548842125001066
50-51	21.850043042096022	21.95019049323685	28.624698492248164	27.575067972418964
52-53	20.978547137487535	21.91908085947821	33.04993735457312	24.052434648461137
54-55	20.84558541512184	21.778022109147937	29.762970160321156	27.61342231540907
56-57	24.436830397095296	22.07633366573764	25.936911367374943	27.54992456979212
58-59	21.200150008097026	22.7177035124055	31.69006281589063	24.392083663606844
60-61	24.46879234958705	27.10373571300724	24.835715564192384	23.591756373213325
62-63	20.39982271770351	32.69153732729892	23.155369181859246	23.753270773138325
64-65	19.960878570150093	33.64442967091974	22.52678411618809	23.867907642742082
66-67	19.7963810546592	33.199093132867965	22.658893519820673	24.345632292652162
68-69	19.89141459340135	33.493995414525216	22.60434512090141	24.010244871172024
70-71	20.258337808006683	32.210403402456386	22.837880453774492	24.693378335762443
72-73	20.30521533832792	29.107110895190363	23.91052357939775	26.677150187083964
74-75	20.626539500711687	28.270560058639525	24.447484381259216	26.65541605938957
76	20.58179276722323	28.038729363232672	24.825487739395026	26.553990130149067
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.5
12	1.0
13	1.0
14	1.0
15	3.5
16	10.5
17	15.0
18	15.0
19	34.0
20	43.5
21	34.0
22	58.5
23	117.5
24	201.5
25	251.0
26	307.0
27	417.0
28	593.0
29	715.0
30	824.5
31	1017.5
32	1419.0
33	1737.0
34	1895.5
35	2279.0
36	2747.5
37	2991.0
38	3443.5
39	5813.5
40	8285.5
41	8620.0
42	8400.0
43	7929.0
44	7105.0
45	6384.5
46	6017.0
47	5916.0
48	5465.0
49	4911.5
50	4708.0
51	4344.5
52	3487.0
53	2905.0
54	2817.0
55	2693.5
56	2338.0
57	1961.0
58	1816.0
59	1697.0
60	1511.0
61	1471.0
62	1498.0
63	1501.0
64	1488.5
65	1487.5
66	1511.0
67	1520.0
68	1540.5
69	1544.5
70	1486.5
71	1445.0
72	1433.5
73	1388.5
74	1260.0
75	1165.0
76	1086.5
77	919.0
78	751.0
79	672.0
80	581.5
81	412.0
82	280.5
83	228.0
84	179.0
85	96.0
86	52.0
87	42.0
88	33.5
89	17.0
90	6.0
91	2.5
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	117327.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.11539543327623
#Duplication Level	Percentage of deduplicated	Percentage of total
1	58.79690032815403	25.350516078992904
2	20.918831297196853	18.038473667612738
3	8.684220930692286	11.232708583701962
4	3.9200569327481913	6.760592191055767
5	2.1646305301862174	4.666445063796057
6	1.3363381172656466	3.4570047815080924
7	0.8539912228679872	2.5774118489350277
8	0.713636183924406	2.4614965012316006
9	0.45467125291582655	1.7642997775448106
>10	2.1231170679634683	13.58681292456127
>50	0.007907326137666548	0.2190459144101528
>100	0.01976831534416637	2.0063582977490264
>500	0.001976831534416637	0.6980490424199033
>1k	0.001976831534416637	0.8557280080458888
>5k	0.001976831534416637	6.325057318434801
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGT	7421	6.325057318434801	No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG	1004	0.8557280080458888	No Hit
ATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTCTT	819	0.6980490424199033	RNA PCR Primer, Index 48 (95% over 23bp)
CTCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC	476	0.40570371696199514	No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC	411	0.35030299930962183	No Hit
CTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTC	382	0.3255857560493322	No Hit
CTTTACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTC	302	0.2574002574002574	RNA PCR Primer, Index 48 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAACCTCGTATGCCGT	149	0.12699549123390183	No Hit
CTTAATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG	140	0.11932462263588091	No Hit
TATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTCT	136	0.11591534770342717	RNA PCR Primer, Index 48 (95% over 22bp)
TCTTAATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC	132	0.11250607277097342	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.00511391239868061	0.0
2	0.0	0.0	0.0	0.00511391239868061	0.0
3	0.0	0.0	0.0	0.00511391239868061	0.0
4	0.0	0.0	0.0	0.006818549864907481	0.0
5	0.0	0.0	0.0	0.007670868598020916	0.0
6	0.0	0.0	0.0	0.00852318733113435	0.0
7	0.0	0.0	0.0	0.009375506064247786	0.0
8	0.0	0.0	0.0	0.009375506064247786	0.0
9	0.0	0.0	0.0	0.01022782479736122	0.0
10	0.0	0.0	0.0	0.011080143530474656	0.0
11	0.0	0.0	0.0	0.011932462263588092	0.0
12	0.0	0.0	0.0	0.012784780996701526	0.0
13	0.0	0.0	0.0	0.012784780996701526	0.0
14	0.0	0.0	0.0	0.012784780996701526	0.0
15	0.0	0.0	0.0	0.013637099729814962	0.0
16	0.0	0.0	0.0	0.014489418462928397	0.0
17	0.0	0.0	0.0	0.014489418462928397	0.0
18	0.0	0.0	0.0	0.015341737196041831	0.0
19	0.0	0.0	0.0	0.015341737196041831	0.0
20	0.0	0.0	0.0	0.016194055929155265	0.0
21	0.0	0.0	0.0	0.0170463746622687	0.0
22	0.0	0.0	0.0	0.018751012128495573	0.0
23	0.0	0.0	0.0	0.021307968327835877	0.0
24	0.0	0.0	0.0	0.022160287060949312	0.0
25	0.0	0.0	0.0	0.023864924527176184	0.0
26	0.0	0.0	0.0	0.029831155658970227	0.0
27	0.0	0.0	0.0	0.04772984905435237	0.0
28	0.0	0.0	0.0	0.07329941104775542	0.0
29	0.0	0.0	0.0	0.11080143530474656	0.0
30	0.0	0.0	0.0	0.1457465033623974	0.0
31	0.0	0.0	0.0	0.17557765902136763	0.0
32	0.0	0.0	0.0	0.22160287060949313	0.0
33	0.0	0.0	0.0	0.28467445685988735	0.0
34	0.0	0.0	0.0	0.3852480673672727	0.0
35	0.0	0.0	0.0	0.5071296462024939	0.0
36	0.0	0.0	0.0	0.695492086220563	0.0
37	0.0	0.0	0.0	1.0108500174725341	0.0
38	0.0	0.0	0.0	1.3500728732516811	0.0
39	0.0	0.0	0.0	1.7182745659566852	0.0
40	0.0	0.0	0.0	2.106079589523298	0.0
41	0.0	0.0	0.0	2.5757072114688007	0.0
42	0.0	0.0	0.0	2.995900346893724	0.0
43	0.0	0.0	0.0	3.439958406845824	0.0
44	0.0	0.0	0.0	3.886573422997264	0.0
45	0.0	0.0	0.0	4.452513061784585	0.0
46	0.0	0.0	0.0	4.9673135765850995	0.0
47	0.0	0.0	0.0	5.539219446504215	0.0
48	0.0	0.0	0.0	6.064247786102091	0.0
49	0.0	0.0	0.0	6.599503950497328	0.0
50	0.0	0.0	0.0	7.120270696429637	0.0
51	0.0	0.0	0.0	7.600126143172501	0.0
52	0.0	0.0	0.0	8.12600680150349	0.0
53	0.0	0.0	0.0	8.642511953770232	0.0
54	0.0	0.0	0.0	9.164983337168767	0.0
55	0.0	0.0	0.0	9.620973859384456	0.0
56	0.0	0.0	0.0	10.087192206397505	0.0
57	0.0	0.0	0.0	10.583241709069524	0.0
58	0.0	0.0	0.0	11.048607737349458	0.0
59	0.0	0.0	0.0	11.516530721828735	0.0
60	0.0	0.0	0.0	12.00405703716962	0.0
61	0.0	0.0	0.0	12.517152914503908	0.0
62	0.0	0.0	0.0	13.008088504777247	0.0
63	0.0	0.0	0.0	13.459817433327368	0.0
64	0.0	0.0	0.0	13.923478824141077	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCAGGAC	15	0.0022199133	70.00001	45
AATATCG	15	0.0022199133	70.00001	36
ATATCGT	15	0.0022199133	70.00001	37
GTTCTGA	25	2.3821658E-4	55.999996	11
ATCGTAT	25	2.3821658E-4	55.999996	39
TCTTTAT	45	2.6757334E-8	54.444443	1
CTTGTTG	20	0.0069365622	52.5	13
CTTAACT	20	0.0069365622	52.5	54
ATGGGAC	20	0.0069365622	52.5	61
TCGTTTA	20	0.0069365622	52.5	32
CAACGAC	20	0.0069365622	52.5	31
GGTCATC	20	0.0069365622	52.5	11
GCTTAAC	20	0.0069365622	52.5	13
ATCCATG	20	0.0069365622	52.5	15
GGTGAAT	20	0.0069365622	52.5	32
AAGGGAT	20	0.0069365622	52.5	30
ATTATAG	20	0.0069365622	52.5	69
CGGTAAA	20	0.0069365622	52.5	58
GCATGCT	20	0.0069365622	52.5	23
CGTTTAT	20	0.0069365622	52.5	33
>>END_MODULE