##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779741_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	125327
Sequences flagged as poor quality	0
Sequence length	76
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.545748322388633	34.0	32.0	34.0	21.0	34.0
2	28.468255044802795	34.0	23.0	34.0	12.0	34.0
3	27.417308321431136	33.0	23.0	34.0	11.0	34.0
4	30.397097193741175	34.0	31.0	34.0	21.0	34.0
5	29.739489495479823	34.0	31.0	34.0	11.0	34.0
6	32.35495144701461	37.0	31.0	38.0	12.0	38.0
7	33.277689564100314	37.0	34.0	38.0	21.0	38.0
8	32.575334923839236	37.0	31.0	38.0	12.0	38.0
9	34.09544631244664	37.0	34.0	38.0	24.0	38.0
10-11	33.99475771382065	38.0	34.0	38.0	22.5	38.0
12-13	32.64793300725302	37.5	30.5	38.0	18.5	38.0
14-15	33.00270891348233	37.0	32.5	38.0	16.0	38.0
16-17	32.543079304539326	37.0	30.0	38.0	18.0	38.0
18-19	33.065237339120856	37.0	32.5	38.0	16.0	38.0
20-21	32.03150558139906	36.5	30.0	38.0	17.0	38.0
22-23	31.947768637244966	36.5	29.0	38.0	17.0	38.0
24-25	33.55800825041691	37.0	33.5	38.0	22.5	38.0
26-27	31.814712711546598	37.0	29.0	38.0	16.0	38.0
28-29	34.40182881581782	37.5	34.5	38.0	25.5	38.0
30-31	34.931658780629874	38.0	36.0	38.0	26.5	38.0
32-33	35.00995795000279	38.0	36.0	38.0	27.0	38.0
34-35	35.087511071038165	38.0	36.0	38.0	27.0	38.0
36-37	32.45066107063921	37.0	30.5	38.0	16.5	38.0
38-39	30.24040709503938	35.0	25.0	38.0	11.0	38.0
40-41	31.96354337054266	36.5	29.5	38.0	16.0	38.0
42-43	33.269403241121225	37.0	32.5	38.0	18.5	38.0
44-45	35.163899239589234	38.0	36.0	38.0	27.0	38.0
46-47	33.40550320361933	37.5	32.0	38.0	19.0	38.0
48-49	32.730173865168716	37.0	30.5	38.0	18.0	38.0
50-51	30.123469005082704	35.0	24.5	38.0	11.0	38.0
52-53	34.2354560469811	37.0	35.0	38.0	25.0	38.0
54-55	33.120855841119635	37.0	30.5	38.0	18.5	38.0
56-57	33.59598889305576	37.5	33.5	38.0	19.0	38.0
58-59	34.960990050029125	38.0	35.5	38.0	26.5	38.0
60-61	35.42550687401757	38.0	37.0	38.0	31.0	38.0
62-63	35.27378378162726	38.0	36.5	38.0	29.0	38.0
64-65	34.927449791345836	38.0	36.0	38.0	27.5	38.0
66-67	32.52615956657384	37.0	31.5	38.0	11.0	38.0
68-69	32.592573826868914	37.0	32.5	38.0	16.0	38.0
70-71	31.965729651232376	37.0	29.5	38.0	14.0	38.0
72-73	30.59657136929792	35.5	26.0	38.0	11.0	38.0
74-75	33.31643221332993	37.0	32.5	38.0	18.0	38.0
76	34.0262593056564	37.0	34.0	38.0	24.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	8.0
13	49.0
14	167.0
15	296.0
16	505.0
17	748.0
18	985.0
19	1101.0
20	1056.0
21	1085.0
22	1062.0
23	1177.0
24	1399.0
25	1707.0
26	1993.0
27	2414.0
28	3042.0
29	3830.0
30	4774.0
31	6492.0
32	8861.0
33	12660.0
34	16756.0
35	21123.0
36	22236.0
37	9801.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	39.044260215276836	21.057713022732532	12.270300892864267	27.627725869126362
2	19.543274793141144	19.339807064718695	38.96287312390786	22.154045018232303
3	21.28352230564842	19.207353563078986	35.32997678074158	24.17914735053101
4	13.80069737566526	18.44055949635753	35.48955931283761	32.2691838151396
5	14.311361478372579	31.685909660328583	38.42747372872565	15.575255132573188
6	28.371380468693896	34.01501671627024	17.248477981600132	20.365124833435733
7	26.374205079512	28.457555036025756	22.421345759493168	22.74689412496908
8	22.592099068835925	32.94900540186872	19.668547080836532	24.790348448458833
9	23.40277833188379	16.724249363664654	21.75030121202933	38.12267109242222
10-11	24.08658948191531	25.35886121905096	27.535168000510662	23.019381298523065
12-13	23.948151635322	21.815331093858468	29.977179697910266	24.25933757290927
14-15	22.596487588468566	24.48195520518324	24.985039137615995	27.936518068732198
16-17	23.285485170793205	25.69877201241552	27.47851620161657	23.537226615174703
18-19	21.49217646636399	25.56113207848269	28.768740973613028	24.177950481540293
20-21	23.296256991709686	25.24316388328134	28.534553607762092	22.92602551724688
22-23	23.024567730816184	24.836228426436442	29.041228147167008	23.09797569558036
24-25	22.129309725757416	26.792311313603612	26.05105045201752	25.027328508621444
26-27	22.066274625579485	25.26151587447238	28.854915540944887	23.817293959003248
28-29	22.047124721727958	26.637915213800696	27.6081770089446	23.706783055526742
30-31	22.36309813527811	26.99538008569582	27.03407884972911	23.60744292929696
32-33	21.983690665219786	27.04285588899439	27.104694120181605	23.86875932560422
34-35	22.036352900811476	26.496684672895704	27.641290384354527	23.82567204193829
36-37	22.671491378553704	25.16975591851716	30.143145531290145	22.015607171638994
38-39	22.71577553121035	24.006798215867292	32.80697694830324	20.470449304619116
40-41	22.068269407230684	25.279867865663423	26.51703144573795	26.134831281367944
42-43	20.945606293935064	25.912213649093967	27.114269072107366	26.027910984863595
44-45	21.31543881206763	26.099723124306813	28.49066841143568	24.09416965218987
46-47	22.190749000614392	25.216832765485492	29.57263797904681	23.019780254853302
48-49	22.248597668499208	25.2786709966727	29.525162175748243	22.947569159079848
50-51	22.677874679837544	23.501320545453094	32.2264954878039	21.594309286905457
52-53	20.901721097608654	26.104909556599935	28.85691032259609	24.136459023195318
54-55	21.646173609836666	25.612996401413902	29.258260390817618	23.48256959793181
56-57	21.75947720762485	25.484532463076594	28.907976732866818	23.848013596431734
58-59	20.561810304244098	26.192280992922512	28.879251877089533	24.366656825743853
60-61	20.31525529215572	26.019133945598316	28.48428511015184	25.181325652094124
62-63	20.318047986467402	26.29401485713374	28.541335865376176	24.846601291022687
64-65	20.553831177639296	26.146002058614666	28.359810735116938	24.940356028629107
66-67	19.431567020673917	24.766810024974667	26.133235456046982	29.668387498304437
68-69	19.95044112189808	25.25427854324032	26.58303107205989	28.212249262801713
70-71	19.772275726698957	24.74287264516026	25.857556631851075	29.627294996289706
72-73	19.30270412600637	23.95932241256872	25.259920049151418	31.478053412273493
74-75	20.756102037071024	25.941337461201496	26.82382886369258	26.4787316380349
76	21.165431231897355	26.25212444245853	26.77555514773353	25.806889177910584
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	1.0
10	1.0
11	0.5
12	1.0
13	3.0
14	6.0
15	12.5
16	31.5
17	45.0
18	63.0
19	103.0
20	159.5
21	194.0
22	252.0
23	364.0
24	532.0
25	646.0
26	742.0
27	979.0
28	1263.0
29	1406.0
30	1543.5
31	1945.0
32	2435.5
33	2662.0
34	2988.5
35	3580.5
36	4061.0
37	4276.0
38	4592.5
39	5215.5
40	5776.5
41	6134.5
42	6238.0
43	6381.5
44	6589.0
45	6660.0
46	6667.0
47	6586.5
48	6390.5
49	6076.0
50	5877.0
51	5662.0
52	5195.5
53	4754.5
54	4565.0
55	4319.5
56	3783.0
57	3282.5
58	3073.0
59	2825.5
60	2328.0
61	1917.5
62	1757.0
63	1635.0
64	1354.0
65	1087.5
66	944.5
67	909.0
68	833.0
69	700.5
70	605.0
71	566.0
72	519.0
73	438.5
74	379.5
75	354.0
76	308.0
77	246.5
78	200.0
79	169.0
80	143.5
81	112.0
82	92.5
83	79.0
84	66.5
85	42.0
86	27.0
87	24.0
88	22.0
89	17.0
90	9.0
91	2.5
92	1.0
93	1.5
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	1.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.017155122200323954
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	125327.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	97.97368814852938
#Duplication Level	Percentage of deduplicated	Percentage of total
1	98.19094053168463	96.20128586662064
2	1.6009092117039707	3.136939597231856
3	0.17113310579696492	0.5029962461772337
4	0.02702125849280004	0.10589489411817568
5	0.006997611677141793	0.034279091212039876
6	0.0019988818386622743	0.011750269554411396
7	9.993988058243238E-4	0.006854035085649147
8	0.0	0.0
9	0.0	0.0
>10	0.0	0.0
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	7.979126604801838E-4	0.0
9	0.0	0.0	0.0	7.979126604801838E-4	0.0
10	0.0	0.0	0.0	7.979126604801838E-4	0.0
11	0.0	0.0	0.0	7.979126604801838E-4	0.0
12	0.0	0.0	0.0	7.979126604801838E-4	0.0
13	0.0	0.0	0.0	0.0015958253209603676	0.0
14	0.0	0.0	0.0	0.0023937379814405516	0.0
15	0.0	0.0	0.0	0.0023937379814405516	0.0
16	0.0	0.0	0.0	0.0023937379814405516	0.0
17	0.0	0.0	0.0	0.0023937379814405516	0.0
18	0.0	0.0	0.0	0.0023937379814405516	0.0
19	0.0	0.0	0.0	0.005585388623361287	0.0
20	0.0	0.0	0.0	0.00638330128384147	0.0
21	0.0	0.0	0.0	0.007181213944321654	0.0
22	0.0	0.0	0.0	0.02074572917248478	0.0
23	0.0	0.0	0.0	0.021543641832964963	0.0
24	0.0	0.0	0.0	0.027926943116806435	0.0
25	0.0	0.0	0.0	0.029522768437766803	0.0
26	0.0	0.0	0.0	0.029522768437766803	0.0
27	0.0	0.0	0.0	0.029522768437766803	0.0
28	0.0	0.0	0.0	0.03111859375872717	0.0
29	0.0	0.0	0.0	0.03750189504256864	0.0
30	0.0	0.0	0.0	0.04149145834496956	0.0
31	0.0	0.0	0.0	0.0446831089868903	0.0
32	0.0	0.0	0.0	0.05346014825217232	0.0
33	0.0	0.0	0.0	0.057449711554573235	0.0
34	0.0	0.0	0.0	0.07181213944321654	0.0
35	0.0	0.0	0.0	0.08697247999234003	0.0
36	0.0	0.0	0.0	0.11011194714626538	0.0
37	0.0	0.0	0.0	0.19548860181764505	0.0
38	0.0	0.0	0.0	0.27926943116806435	0.0
39	0.0	0.0	0.0	0.38938137831432973	0.0
40	0.0	0.0	0.0	0.5346014825217231	0.0
41	0.0	0.0	0.0	0.729292171678888	0.0
42	0.0	0.0	0.0	0.9231849481755727	0.0
43	0.0	0.0	0.0	1.1434088424681035	0.0
44	0.0	0.0	0.0	1.4346469635433705	0.0
45	0.0	0.0	0.0	1.6556687704963815	0.0
46	0.0	0.0	0.0	1.9157882978129215	0.0
47	0.0	0.0	0.0	2.2509116152145987	0.0
48	0.0	0.0	0.0	2.5142227931730594	0.0
49	0.0	0.0	0.0	2.730457124163189	0.0
50	0.0	0.0	0.0	3.0935073846816725	0.0
51	0.0	0.0	0.0	3.4972511908846458	0.0
52	0.0	0.0	0.0	3.8666847526869708	0.0
53	0.0	0.0	0.0	4.3366553097097995	0.0
54	0.0	0.0	0.0	4.845723587096156	0.0
55	0.0	0.0	0.0	5.211167585596081	0.0
56	0.0	0.0	0.0	5.614113479138574	0.0
57	0.0	0.0	0.0	5.981951215619938	0.0
58	0.0	0.0	0.0	6.2604227341275225	0.0
59	0.0	0.0	0.0	6.59873770217112	0.0
60	0.0	0.0	0.0	6.815769945821731	0.0
61	0.0	0.0	0.0	7.059133307268186	0.0
62	0.0	0.0	0.0	7.24903652046247	0.0
63	0.0	0.0	0.0	7.505964397137089	0.0
64	0.0	0.0	0.0	7.7341674180344215	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAATAG	20	7.901122E-5	70.00001	62
ATTCGGT	15	0.0022202684	70.0	1
CAACGAT	20	0.0069376617	52.500004	39
GAGCGTC	20	0.0069376617	52.500004	23
TGCGAAC	20	0.0069376617	52.500004	26
TGAGCGT	20	0.0069376617	52.500004	22
CGGTGGT	30	5.85926E-4	46.666664	46
TACACCG	30	5.85926E-4	46.666664	5
TACAGAT	40	4.571292E-5	43.750004	5
ACACCGA	35	0.0012514922	40.0	6
GTAATAG	35	0.0012514922	40.0	51
GGTATAG	35	0.0012514922	40.0	1
TCGTATG	35	0.0012514922	40.0	9
CTCTCGG	35	0.0012514922	40.0	42
GCTCTCG	40	0.0024112435	35.000004	41
CCGGTGG	40	0.0024112435	35.000004	45
TCGGTGG	55	2.978365E-4	31.81818	45
TACCATT	45	0.0042939675	31.111109	27
AGTGTTG	45	0.0042939675	31.111109	45
CCACAGT	45	0.0042939675	31.111109	70
>>END_MODULE