FastQCFastQC Report
Wed 25 May 2016
SRR1779741_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779741_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences125327
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT19111.5248110941776314No Hit
ATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTCTT5080.4053396315239334TruSeq Adapter, Index 2 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCG2550.2034677284224469No Hit
CTTTACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTC2390.19070112585476393No Hit
CTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTC2280.18192408658948192No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC1730.13803889026307178No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTAGC207.901122E-570.000014
TATCCGG150.002220268470.053
GGGGATC200.006937661752.50000440
CCCGTCA200.006937661752.50000412
GCGGCAA200.006937661752.50000436
TTCGGCA200.006937661752.5000043
TCCGGAA350.001251492240.055
CGTCTTC4600.036.5217448
ATCTCGT4350.036.20689837
TATCTCG4200.035.83333236
GCCGTCT4600.035.7608746
TCTCGTA4350.035.402338
TATGCCG4550.035.38461343
TGCCGTC4650.035.37634345
CTCGTAT4600.035.00000439
CATACTT400.002411243535.00000411
TCGTATG4600.035.00000440
CTTTATA806.175651E-835.0000042
ATGCCGT4600.035.00000444
CCGTCTT4800.035.047
Produced by FastQC (version 0.11.5)