##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779741_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	125327
Sequences flagged as poor quality	0
Sequence length	76
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.34440304164306	34.0	34.0	34.0	33.0	34.0
2	33.38988406329043	34.0	34.0	34.0	34.0	34.0
3	33.43180639447206	34.0	34.0	34.0	34.0	34.0
4	33.48790763363042	34.0	34.0	34.0	34.0	34.0
5	33.48099770999067	34.0	34.0	34.0	34.0	34.0
6	36.821578749990024	38.0	37.0	38.0	34.0	38.0
7	36.87764807264197	38.0	37.0	38.0	35.0	38.0
8	36.542309318821964	38.0	37.0	38.0	34.0	38.0
9	36.57661956322261	38.0	37.0	38.0	34.0	38.0
10-11	36.49459813128855	38.0	37.0	38.0	34.0	38.0
12-13	36.5033233062309	38.0	37.0	38.0	34.0	38.0
14-15	36.54860086014985	38.0	37.0	38.0	34.0	38.0
16-17	36.49078410877145	38.0	37.0	38.0	34.0	38.0
18-19	36.52350251741444	38.0	37.0	38.0	34.0	38.0
20-21	36.54613132046566	38.0	37.0	38.0	34.0	38.0
22-23	36.37860955739785	38.0	37.0	38.0	34.0	38.0
24-25	36.382080477470936	38.0	37.0	38.0	34.0	38.0
26-27	36.24630765916363	38.0	37.0	38.0	34.0	38.0
28-29	36.16902183886952	38.0	37.0	38.0	33.5	38.0
30-31	36.06062141437998	38.0	37.0	38.0	33.0	38.0
32-33	36.08885555387107	38.0	37.0	38.0	33.5	38.0
34-35	36.09536253161729	38.0	37.0	38.0	33.5	38.0
36-37	36.1548668682726	38.0	37.0	38.0	34.0	38.0
38-39	36.12856367741987	38.0	37.0	38.0	34.0	38.0
40-41	36.16606956202574	38.0	37.0	38.0	34.0	38.0
42-43	36.146089829007316	38.0	37.0	38.0	34.0	38.0
44-45	36.11968689907202	38.0	37.0	38.0	34.0	38.0
46-47	36.180352198648336	38.0	37.0	38.0	34.0	38.0
48-49	36.161573324183934	38.0	37.0	38.0	34.0	38.0
50-51	36.14484907482027	38.0	37.0	38.0	34.0	38.0
52-53	36.09398613227796	38.0	37.0	38.0	34.0	38.0
54-55	36.0864019724401	38.0	37.0	38.0	34.0	38.0
56-57	36.087247759860205	38.0	37.0	38.0	33.5	38.0
58-59	36.07619267994925	38.0	37.0	38.0	34.0	38.0
60-61	36.15177894627654	38.0	37.0	38.0	34.0	38.0
62-63	36.06175445035787	38.0	37.0	38.0	33.0	38.0
64-65	36.05425407134935	38.0	37.0	38.0	33.5	38.0
66-67	36.04554884422351	38.0	37.0	38.0	33.0	38.0
68-69	35.95198560565561	38.0	37.0	38.0	32.0	38.0
70-71	35.79964014139013	38.0	37.0	38.0	31.5	38.0
72-73	35.63396554613132	38.0	37.0	38.0	31.0	38.0
74-75	35.6287870929648	38.0	37.0	38.0	31.0	38.0
76	34.81003295379288	38.0	35.0	38.0	27.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	2.0
21	8.0
22	7.0
23	35.0
24	54.0
25	115.0
26	213.0
27	406.0
28	737.0
29	1136.0
30	1835.0
31	2880.0
32	4460.0
33	6814.0
34	10280.0
35	15215.0
36	24456.0
37	56672.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	40.34086828855713	20.906907529901776	12.381210752671013	26.37101342887008
2	19.83211917623497	22.36628978592003	34.69723204098079	23.104358996864203
3	20.211127689963057	24.074620792008105	30.438772171998053	25.27547934603078
4	14.71829693521747	18.685518683124947	36.09916458544448	30.497019796213102
5	14.315351041674978	35.717762333734946	35.138477742226335	14.828408882363735
6	30.43079304539325	37.0079871057314	18.072721759876163	14.488498088999178
7	27.373989643093665	30.741181070320046	22.063082974937565	19.821746311648727
8	24.025948119718816	36.3153989164346	20.508749112322167	19.14990385152441
9	24.39139211821874	18.005697096395828	21.545237658285924	36.05767312709951
10-11	24.70457283745721	26.72129708682088	26.921573164601405	21.652556911120506
12-13	24.63794713030712	23.078027879068358	27.361621996856222	24.9224029937683
14-15	22.64835191139978	24.935568552666226	23.735906867634267	28.68017266829973
16-17	23.95293911128488	28.18746160045321	24.382615078953457	23.476984209308448
18-19	23.121115162734288	26.82662155800426	26.311169979334064	23.74109329992739
20-21	22.727744221117558	26.498280498216666	25.632146305265422	25.141828975400355
22-23	22.596886544798807	27.841566462135052	25.543179043621883	24.018367949444254
24-25	23.844422989459574	28.02668219936646	25.54158321830092	22.587311592873043
26-27	23.410358502158353	27.991972998635568	26.245342184844446	22.35232631436163
28-29	23.6078418856272	26.81824347506922	26.409313236573123	23.164601402730455
30-31	23.837241775515253	27.573068851883477	26.158768661182346	22.430920711418928
32-33	22.4843808596711	27.81643221332993	26.52660639766371	23.17258052933526
34-35	23.31022046326809	26.619962178939893	26.999768605328462	23.070048752463556
36-37	22.638776959474015	27.47213290033273	27.74422111755647	22.144869022636783
38-39	21.848045512938157	26.8409839858929	28.20661150430474	23.104358996864203
40-41	22.318813982621464	26.009957950002793	28.398908455480466	23.27231961189528
42-43	23.157819145116378	26.126852154763142	28.209005242286178	22.506323457834306
44-45	22.375465781515555	26.542165694543073	28.623520869405635	22.458847654535735
46-47	23.148643149520854	25.83202342671571	27.497666105468095	23.52166731829534
48-49	22.496349549578305	25.501687585276915	28.058598705785663	23.943364159359117
50-51	21.46903699921007	25.586665283618053	28.982182610291478	23.962115106880404
52-53	21.23963710932201	25.412720323633376	30.42600556943037	22.921636997614243
54-55	21.203731039600406	25.6975751434248	29.438588652086146	23.660105164888652
56-57	22.248996624829445	25.472962729499628	28.254486263933547	24.023554381737373
58-59	20.845069298714563	25.926975033312853	29.927709112960493	23.300246555012087
60-61	21.632210138278264	27.442211175564722	28.067774701381186	22.857803984775828
62-63	20.211127689963057	28.892018479657217	27.70392652820222	23.192927302177505
64-65	20.172029969599528	29.091895601107503	27.0807567403672	23.65531768892577
66-67	19.910314616962026	29.29496437319971	26.860133889744432	23.934587120093834
68-69	20.211526646293297	29.56106824546985	26.459182777853137	23.76822233038372
70-71	20.510743893973366	28.807838693976556	26.304786678050217	24.376630733999857
72-73	20.299695995276355	27.946890933318443	26.590439410502125	25.162973660903077
74-75	20.755304124410543	27.591021886744276	26.366225952907197	25.287448035937988
76	21.104789869700863	27.967636662490925	25.737470776448813	25.190102691359407
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	2.0
11	3.0
12	2.0
13	4.0
14	11.5
15	35.5
16	61.5
17	69.0
18	105.5
19	179.5
20	250.0
21	283.0
22	377.5
23	560.0
24	735.0
25	822.0
26	949.5
27	1193.5
28	1511.5
29	1713.0
30	1875.5
31	2237.0
32	2730.0
33	3024.0
34	3194.0
35	3687.0
36	4233.0
37	4456.0
38	4714.5
39	5163.5
40	5609.5
41	6437.5
42	7010.0
43	7158.5
44	7241.5
45	6987.5
46	6799.0
47	6424.0
48	5872.0
49	5450.0
50	5205.0
51	4942.5
52	4545.0
53	4168.0
54	3926.0
55	3889.5
56	3586.5
57	2984.5
58	2649.0
59	2497.5
60	2127.0
61	1771.5
62	1635.0
63	1464.5
64	1190.0
65	1045.5
66	925.0
67	845.0
68	800.0
69	674.0
70	587.5
71	582.0
72	534.0
73	483.5
74	453.0
75	425.0
76	380.0
77	297.0
78	233.0
79	207.0
80	187.0
81	160.0
82	133.0
83	113.0
84	96.0
85	65.0
86	42.0
87	33.0
88	26.0
89	20.0
90	13.0
91	5.0
92	5.0
93	4.5
94	2.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	125327.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.84237235392214
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.97680428228635	44.72619627055622
2	20.662031624930783	26.795502964245532
3	6.589552697963452	12.818466890614152
4	2.0513136036424044	5.320481620081866
5	0.7961607087922229	2.5812474566533945
6	0.3285547283578416	1.2782560820892546
7	0.21903648557189442	0.9941991749583091
8	0.12674583153879285	0.6574800322356714
9	0.07752414938780533	0.45241647849226424
>10	0.15627884082938534	1.3101725885084619
>50	0.008613794376422814	0.421297884733537
>100	0.0049221682150987515	0.7141318311297645
>500	0.0012305420537746879	0.4053396315239334
>1k	0.0012305420537746879	1.5248110941776314
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT	1911	1.5248110941776314	No Hit
ATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTCTT	508	0.4053396315239334	TruSeq Adapter, Index 2 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCG	255	0.2034677284224469	No Hit
CTTTACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTC	239	0.19070112585476393	No Hit
CTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTC	228	0.18192408658948192	No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC	173	0.13803889026307178	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	7.979126604801838E-4	0.0
2	0.0	0.0	0.0	7.979126604801838E-4	0.0
3	0.0	0.0	0.0	7.979126604801838E-4	0.0
4	0.0	0.0	0.0	7.979126604801838E-4	0.0
5	0.0	0.0	0.0	7.979126604801838E-4	0.0
6	0.0	0.0	0.0	7.979126604801838E-4	0.0
7	0.0	0.0	0.0	7.979126604801838E-4	0.0
8	0.0	0.0	0.0	0.0015958253209603676	0.0
9	0.0	0.0	0.0	0.0023937379814405516	0.0
10	0.0	0.0	0.0	0.0023937379814405516	0.0
11	0.0	0.0	0.0	0.0023937379814405516	0.0
12	0.0	0.0	0.0	0.0023937379814405516	0.0
13	0.0	0.0	0.0	0.003191650641920735	0.0
14	0.0	0.0	0.0	0.003191650641920735	0.0
15	0.0	0.0	0.0	0.003191650641920735	0.0
16	0.0	0.0	0.0	0.003989563302400919	0.0
17	0.0	0.0	0.0	0.003989563302400919	0.0
18	0.0	0.0	0.0	0.004787475962881103	0.0
19	0.0	0.0	0.0	0.00638330128384147	0.0
20	0.0	0.0	0.0	0.007181213944321654	0.0
21	0.0	0.0	0.0	0.008777039265282022	0.0
22	0.0	0.0	0.0	0.011170777246722574	0.0
23	0.0	0.0	0.0	0.01276660256768294	0.0
24	0.0	0.0	0.0	0.01675616587008386	0.0
25	0.0	0.0	0.0	0.019947816512004595	0.0
26	0.0	0.0	0.0	0.019947816512004595	0.0
27	0.0	0.0	0.0	0.019947816512004595	0.0
28	0.0	0.0	0.0	0.0247352924748857	0.0
29	0.0	0.0	0.0	0.03510815706112809	0.0
30	0.0	0.0	0.0	0.043087283665929926	0.0
31	0.0	0.0	0.0	0.05106641027073176	0.0
32	0.0	0.0	0.0	0.06223718751745434	0.0
33	0.0	0.0	0.0	0.07340796476417691	0.0
34	0.0	0.0	0.0	0.10213282054146353	0.0
35	0.0	0.0	0.0	0.1324535016397105	0.0
36	0.0	0.0	0.0	0.19469068915716486	0.0
37	0.0	0.0	0.0	0.3486878326298403	0.0
38	0.0	0.0	0.0	0.4875246355533923	0.0
39	0.0	0.0	0.0	0.6606716828775923	0.0
40	0.0	0.0	0.0	0.9056308696450086	0.0
41	0.0	0.0	0.0	1.1793149121897117	0.0
42	0.0	0.0	0.0	1.484117548493142	0.0
43	0.0	0.0	0.0	1.809665913969057	0.0
44	0.0	0.0	0.0	2.204632680906748	0.0
45	0.0	0.0	0.0	2.664230373343334	0.0
46	0.0	0.0	0.0	3.160532048162008	0.0
47	0.0	0.0	0.0	3.6568337229806827	0.0
48	0.0	0.0	0.0	4.117229328077749	0.0
49	0.0	0.0	0.0	4.576029107853854	0.0
50	0.0	0.0	0.0	5.033233062309	0.0
51	0.0	0.0	0.0	5.5271409991462335	0.0
52	0.0	0.0	0.0	6.030623887909229	0.0
53	0.0	0.0	0.0	6.537298427314147	0.0
54	0.0	0.0	0.0	7.094241464329315	0.0
55	0.0	0.0	0.0	7.669536492535527	0.0
56	0.0	0.0	0.0	8.252012734686062	0.0
57	0.0	0.0	0.0	8.85683053133004	0.0
58	0.0	0.0	0.0	9.334780214957672	0.0
59	0.0	0.0	0.0	9.858210920232672	0.0
60	0.0	0.0	0.0	10.358502158353746	0.0
61	0.0	0.0	0.0	10.904274418122192	0.0
62	0.0	0.0	0.0	11.41972599679239	0.0
63	0.0	0.0	0.0	11.988637723714762	0.0
64	0.0	0.0	0.0	12.507280953026882	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGTAGC	20	7.901122E-5	70.00001	4
TATCCGG	15	0.0022202684	70.0	53
GGGGATC	20	0.0069376617	52.500004	40
CCCGTCA	20	0.0069376617	52.500004	12
GCGGCAA	20	0.0069376617	52.500004	36
TTCGGCA	20	0.0069376617	52.500004	3
TCCGGAA	35	0.0012514922	40.0	55
CGTCTTC	460	0.0	36.52174	48
ATCTCGT	435	0.0	36.206898	37
TATCTCG	420	0.0	35.833332	36
GCCGTCT	460	0.0	35.76087	46
TCTCGTA	435	0.0	35.4023	38
TATGCCG	455	0.0	35.384613	43
TGCCGTC	465	0.0	35.376343	45
CTCGTAT	460	0.0	35.000004	39
CATACTT	40	0.0024112435	35.000004	11
TCGTATG	460	0.0	35.000004	40
CTTTATA	80	6.175651E-8	35.000004	2
ATGCCGT	460	0.0	35.000004	44
CCGTCTT	480	0.0	35.0	47
>>END_MODULE