FastQCFastQC Report
Wed 25 May 2016
SRR1779740_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779740_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56029
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAAGTA150.002206780570.05630567
CGTATCA150.002214622569.9937656
CGCCGTA150.002214622569.9937653
TCCTTAG150.002214622569.9937638
GCCGTAT150.002214622569.9937654
CGTGTAG207.866522E-569.9937535
GTAGCTC308.281275E-658.3281338
TCTCGGT509.549694E-1055.99543
CTCTCGG252.3724277E-455.99542
TCGGTGG252.3724277E-455.99545
ACGATAC200.006895734552.5422368
TTGTCGC200.006920168652.49531649
AGACGTC200.006920168652.49531653
TATCTCG200.006920168652.49531641
GTAGATC200.006920168652.49531638
CCTACGG305.833926E-446.66250226
TACGGTG305.833926E-446.66250228
GCTCTCG305.833926E-446.66250241
ACCTACG305.833926E-446.66250225
CTCGGTT305.833926E-446.66250244
Produced by FastQC (version 0.11.5)