##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779740_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	56029
Sequences flagged as poor quality	0
Sequence length	76
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.543093755019722	33.0	23.0	34.0	12.0	34.0
2	27.364900319477414	32.0	23.0	34.0	12.0	34.0
3	26.262078566456655	32.0	21.0	34.0	11.0	34.0
4	28.642363775901764	33.0	23.0	34.0	12.0	34.0
5	28.112334683824447	33.0	23.0	34.0	11.0	34.0
6	30.441914008816862	36.0	26.0	38.0	11.0	38.0
7	31.248014421103356	36.0	27.0	38.0	12.0	38.0
8	30.665744525156615	36.0	26.0	38.0	11.0	38.0
9	32.19937889307323	37.0	31.0	38.0	12.0	38.0
10-11	31.893644362740723	37.0	29.5	38.0	11.0	38.0
12-13	30.791500829927358	35.5	26.5	38.0	11.0	38.0
14-15	30.962697888593407	35.5	26.5	38.0	11.0	38.0
16-17	30.543093755019722	35.5	26.5	38.0	11.0	38.0
18-19	31.07344410930054	36.0	26.5	38.0	11.5	38.0
20-21	30.00454229059951	35.0	24.0	37.5	11.0	38.0
22-23	29.972478537900017	35.5	24.5	37.5	11.0	38.0
24-25	31.276071320209176	36.0	27.0	38.0	11.0	38.0
26-27	29.836888040122076	35.0	24.0	38.0	11.0	38.0
28-29	32.094424315979225	37.0	31.0	38.0	11.0	38.0
30-31	32.645112352531726	37.0	31.5	38.0	11.0	38.0
32-33	32.73204947437934	37.0	32.0	38.0	11.0	38.0
34-35	32.8018436880901	37.0	33.0	38.0	11.0	38.0
36-37	30.496028842206712	35.5	25.0	38.0	11.0	38.0
38-39	28.57803101965054	34.0	21.0	37.0	11.0	38.0
40-41	29.84424137500223	34.0	24.5	37.0	11.0	38.0
42-43	31.092184404504806	36.0	27.5	37.5	11.0	38.0
44-45	32.88969997679773	37.0	33.0	38.0	16.0	38.0
46-47	31.365846258187723	36.0	28.5	38.0	16.0	38.0
48-49	30.709561120134218	35.5	27.0	38.0	11.0	38.0
50-51	28.286128611968802	34.0	21.0	37.5	11.0	38.0
52-53	31.875546591943454	36.5	29.0	38.0	11.0	38.0
54-55	30.989442967034925	36.0	27.0	38.0	11.0	38.0
56-57	31.438005318674257	36.5	28.0	38.0	11.0	38.0
58-59	32.66423637759018	37.0	32.0	38.0	11.0	38.0
60-61	33.190856520730335	37.0	34.0	38.0	20.0	38.0
62-63	33.09031929893448	37.0	34.0	38.0	16.0	38.0
64-65	32.791099252172984	37.5	32.5	38.0	16.0	38.0
66-67	30.419729068875046	36.0	24.5	38.0	11.0	38.0
68-69	30.470934694533188	35.5	25.0	38.0	11.0	38.0
70-71	30.01648253582966	35.0	24.0	38.0	11.0	38.0
72-73	28.777213585821627	34.0	22.0	37.0	11.0	38.0
74-75	31.132877616948367	35.5	28.0	37.5	11.0	38.0
76	31.603151939174356	36.0	27.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	1.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	2.0
12	11.0
13	69.0
14	198.0
15	334.0
16	526.0
17	854.0
18	1134.0
19	1327.0
20	1233.0
21	1151.0
22	1030.0
23	1035.0
24	1148.0
25	1254.0
26	1386.0
27	1594.0
28	1851.0
29	2147.0
30	2619.0
31	3222.0
32	3993.0
33	4960.0
34	6274.0
35	7198.0
36	6725.0
37	2753.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.46734726659408	20.751753556194114	13.739313569758519	30.041585607453282
2	17.221438897713686	18.26197147905549	43.79339270734798	20.723196915882845
3	18.10490995734352	20.243088400649665	39.36711345910154	22.284888182905284
4	13.632226168591266	20.264505880883114	34.737368148637316	31.365899801888308
5	13.194952613824984	30.528833282764285	40.15420585768084	16.12200824572989
6	25.49572542790341	35.69936996912313	17.47487908047618	21.33002552249728
7	23.612771957379213	27.55358832033411	23.200485462885293	25.63315425940138
8	20.25951310059256	34.81830513314772	20.37909616620261	24.54308560005711
9	22.056828728492896	16.425715713571787	22.97065752837867	38.54679802955665
10-11	23.3775969158278	25.43906618119512	28.408117369886487	22.7752195330906
12-13	22.30224173627472	21.162632969229673	31.31291497108589	25.222210323409723
14-15	23.232133933033484	24.727814664096524	24.702827157849647	27.337224245020348
16-17	24.652852145355894	24.609124009423862	26.574212893553224	24.163810951667024
18-19	20.910973084886127	24.582351681302207	29.38530734632684	25.12136788748483
20-21	24.6216177625473	23.675662168915544	27.685264510601844	24.017455557935318
22-23	24.841150853144857	23.58552866423931	27.246198329406724	24.32712215320911
24-25	21.482625069160612	26.237305680986633	25.56443984365239	26.715629406200357
26-27	23.35082458770615	25.082101806239738	27.786999357464126	23.78007424858999
28-29	22.052366673805953	26.636681659170414	26.78303705290212	24.52791461412151
30-31	21.842650103519667	26.186906546726636	27.4568073106304	24.513636039123295
32-33	22.24423502534447	25.85046762333119	27.45145284500607	24.45384450631827
34-35	21.7551938316556	25.65770686085529	27.59959306061255	24.987506246876563
36-37	23.802384522024703	24.053151995430856	30.20632540872421	21.93813807382023
38-39	23.616763047047904	22.35935603626758	33.87324195045334	20.15063896623117
40-41	22.21210823159849	25.539908617120012	26.1895837795388	26.058399371742702
42-43	20.402298850574713	26.239558792032557	27.09448847005069	26.26365388734204
44-45	20.978796316127653	25.002677232812164	28.68565717141429	25.33286927964589
46-47	23.697080031412867	24.19593774541301	28.945348754194335	23.161633468979794
48-49	23.620332690797458	24.218248018847717	29.46383950881702	22.6975797815378
50-51	26.427857499821517	22.179089026915115	30.49368173056329	20.89937174270008
52-53	20.895802098950526	25.662168915542228	28.858784893267654	24.583244092239596
54-55	23.259798672092526	24.449382451631326	27.96191190119226	24.328906975083886
56-57	23.15181694866852	24.518990504747627	27.886949382451633	24.44224316413222
58-59	20.64968096024274	25.8569452501004	28.60916514211771	24.884208647539154
60-61	20.5656100521168	25.144570571856928	28.38223745270222	25.907581923324052
62-63	20.556685942742913	26.14228599985721	28.126115513671735	25.174912543728134
64-65	20.51295780681088	26.587599057614046	27.904797601199398	24.99464553437567
66-67	18.96105518669237	27.067716141929036	24.784036553151996	29.1871921182266
68-69	19.317005248313045	27.62951194258988	25.270448784319328	27.78303402477775
70-71	19.199328906975083	27.136431784107945	24.78939101877633	28.87484829014064
72-73	18.354215749268224	26.971335760691083	24.236988648532876	30.43745984150782
74-75	20.609338188048834	26.77232812165346	25.912043978010995	26.70628971228671
76	21.12872135360891	25.710359106161206	26.6224030841722	26.538516456057685
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.5
6	0.5
7	0.0
8	0.0
9	0.0
10	0.0
11	0.5
12	1.0
13	3.0
14	7.0
15	15.0
16	23.0
17	25.0
18	29.0
19	58.0
20	94.5
21	106.0
22	147.5
23	217.0
24	307.5
25	370.0
26	424.5
27	555.5
28	710.5
29	789.0
30	869.5
31	1039.0
32	1265.5
33	1403.0
34	1517.0
35	1704.5
36	1915.0
37	2052.0
38	2164.0
39	2327.5
40	2465.5
41	2625.0
42	2698.0
43	2645.5
44	2650.5
45	2686.5
46	2665.0
47	2636.0
48	2493.0
49	2322.5
50	2266.0
51	2164.5
52	1970.5
53	1773.5
54	1669.0
55	1588.5
56	1443.5
57	1311.5
58	1244.0
59	1154.5
60	990.5
61	872.5
62	829.0
63	809.0
64	759.0
65	694.5
66	645.5
67	631.0
68	614.0
69	582.5
70	533.0
71	498.0
72	473.0
73	410.0
74	347.0
75	322.0
76	276.5
77	212.0
78	161.5
79	130.0
80	118.5
81	78.0
82	44.5
83	40.0
84	35.5
85	22.5
86	10.0
87	6.0
88	4.5
89	2.5
90	2.5
91	1.5
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.001784790019454211
9	0.001784790019454211
10-11	0.001784790019454211
12-13	0.001784790019454211
14-15	0.001784790019454211
16-17	0.001784790019454211
18-19	0.001784790019454211
20-21	0.001784790019454211
22-23	0.001784790019454211
24-25	0.0
26-27	0.001784790019454211
28-29	0.001784790019454211
30-31	0.001784790019454211
32-33	0.001784790019454211
34-35	0.001784790019454211
36-37	0.001784790019454211
38-39	0.001784790019454211
40-41	0.001784790019454211
42-43	0.001784790019454211
44-45	0.001784790019454211
46-47	0.001784790019454211
48-49	0.001784790019454211
50-51	0.001784790019454211
52-53	0.001784790019454211
54-55	0.001784790019454211
56-57	0.001784790019454211
58-59	0.0026771850291813165
60-61	0.001784790019454211
62-63	0.001784790019454211
64-65	0.001784790019454211
66-67	0.001784790019454211
68-69	0.019632690213996325
70-71	0.001784790019454211
72-73	0.001784790019454211
74-75	0.001784790019454211
76	0.001784790019454211
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	56029.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	98.79883631690731
#Duplication Level	Percentage of deduplicated	Percentage of total
1	98.87817038803381	97.69048171482625
2	1.0369246332827515	2.0489389423334345
3	0.07587253414264036	0.22488354245123063
4	0.009032444540790519	0.03569580038908422
5	0.0	0.0
6	0.0	0.0
7	0.0	0.0
8	0.0	0.0
9	0.0	0.0
>10	0.0	0.0
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0017847900194542113	0.0
9	0.0	0.0	0.0	0.005354370058362634	0.0
10	0.0	0.0	0.0	0.007139160077816845	0.0
11	0.0	0.0	0.0	0.007139160077816845	0.0
12	0.0	0.0	0.0	0.007139160077816845	0.0
13	0.0	0.0	0.0	0.007139160077816845	0.0
14	0.0	0.0	0.0	0.010708740116725268	0.0
15	0.0	0.0	0.0	0.010708740116725268	0.0
16	0.0	0.0	0.0	0.010708740116725268	0.0
17	0.0	0.0	0.0	0.010708740116725268	0.0
18	0.0	0.0	0.0	0.010708740116725268	0.0
19	0.0	0.0	0.0	0.01427832015563369	0.0
20	0.0	0.0	0.0	0.01427832015563369	0.0
21	0.0	0.0	0.0	0.01427832015563369	0.0
22	0.0	0.0	0.0	0.024987060272358957	0.0
23	0.0	0.0	0.0	0.024987060272358957	0.0
24	0.0	0.0	0.0	0.03569580038908422	0.0
25	0.0	0.0	0.0	0.03569580038908422	0.0
26	0.0	0.0	0.0	0.03748059040853843	0.0
27	0.0	0.0	0.0	0.04283496046690107	0.0
28	0.0	0.0	0.0	0.04461975048635528	0.0
29	0.0	0.0	0.0	0.051758910564172124	0.0
30	0.0	0.0	0.0	0.062467650680897394	0.0
31	0.0	0.0	0.0	0.0642524407003516	0.0
32	0.0	0.0	0.0	0.07496118081707687	0.0
33	0.0	0.0	0.0	0.08745471095325635	0.0
34	0.0	0.0	0.0	0.0963786610505274	0.0
35	0.0	0.0	0.0	0.14813757161469954	0.0
36	0.0	0.0	0.0	0.16420068178978744	0.0
37	0.0	0.0	0.0	0.2409466526263185	0.0
38	0.0	0.0	0.0	0.28913598315158223	0.0
39	0.0	0.0	0.0	0.3408948937157543	0.0
40	0.0	0.0	0.0	0.4247800246301023	0.0
41	0.0	0.0	0.0	0.5300826357779007	0.0
42	0.0	0.0	0.0	0.6407396169840618	0.0
43	0.0	0.0	0.0	0.794231558657124	0.0
44	0.0	0.0	0.0	0.9512930803690945	0.0
45	0.0	0.0	0.0	1.0744435917114352	0.0
46	0.0	0.0	0.0	1.2261507433650431	0.0
47	0.0	0.0	0.0	1.3493012547073837	0.0
48	0.0	0.0	0.0	1.4349711756411858	0.0
49	0.0	0.0	0.0	1.5509825269057096	0.0
50	0.0	0.0	0.0	1.7026896785593175	0.0
51	0.0	0.0	0.0	1.927573221010548	0.0
52	0.0	0.0	0.0	2.122115333131057	0.0
53	0.0	0.0	0.0	2.384479465990826	0.0
54	0.0	0.0	0.0	2.627210908636599	0.0
55	0.0	0.0	0.0	2.8485248710489213	0.0
56	0.0	0.0	0.0	3.100180263791965	0.0
57	0.0	0.0	0.0	3.260811365542844	0.0
58	0.0	0.0	0.0	3.412518517196452	0.0
59	0.0	0.0	0.0	3.574934408966785	0.0
60	0.0	0.0	0.0	3.6713130700173124	0.0
61	0.0	0.0	0.0	3.80160274143747	0.0
62	0.0	0.0	0.0	3.905120562565814	0.0
63	0.0	0.0	0.0	4.053258134180513	0.0
64	0.0	0.0	0.0	4.1853325956201255	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCAAGTA	15	0.0022067805	70.056305	67
CGTATCA	15	0.0022146225	69.99376	56
CGCCGTA	15	0.0022146225	69.99376	53
TCCTTAG	15	0.0022146225	69.99376	38
GCCGTAT	15	0.0022146225	69.99376	54
CGTGTAG	20	7.866522E-5	69.99375	35
GTAGCTC	30	8.281275E-6	58.32813	38
TCTCGGT	50	9.549694E-10	55.995	43
CTCTCGG	25	2.3724277E-4	55.995	42
TCGGTGG	25	2.3724277E-4	55.995	45
ACGATAC	20	0.0068957345	52.54223	68
TTGTCGC	20	0.0069201686	52.495316	49
AGACGTC	20	0.0069201686	52.495316	53
TATCTCG	20	0.0069201686	52.495316	41
GTAGATC	20	0.0069201686	52.495316	38
CCTACGG	30	5.833926E-4	46.662502	26
TACGGTG	30	5.833926E-4	46.662502	28
GCTCTCG	30	5.833926E-4	46.662502	41
ACCTACG	30	5.833926E-4	46.662502	25
CTCGGTT	30	5.833926E-4	46.662502	44
>>END_MODULE