SRR1779740_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1779740_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	56029
Sequences flagged as poor quality	0
Sequence length	76
%GC	45

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT	3115	5.559620910599868	No Hit
ATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTCTT	479	0.8549144193185673	RNA PCR Primer, Index 47 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCG	462	0.8245729889878456	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC	232	0.414071284513377	No Hit
CTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTC	217	0.3872994342215638	No Hit
CTTTACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTC	192	0.3426796837352085	No Hit
TCTTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC	184	0.3284013635795749	No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGACCTCGTATGCCGT	102	0.18204858198432955	No Hit
CTTAATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCG	93	0.16598547180924164	No Hit
TATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTCT	83	0.14813757161469954	No Hit
TCTTAATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC	79	0.1409984115368827	No Hit
TTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTC	77	0.13742883149797425	No Hit
CTTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCG	60	0.10708740116725267	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATATCGT	20	7.863046E-5	70.0	37
GATATCG	20	7.863046E-5	70.0	36
TATCGTA	20	7.863046E-5	70.0	38
AGATATC	25	2.3713808E-4	56.000004	35
GCGTTGA	20	0.006917726	52.5	26
AGAGTAC	20	0.006917726	52.5	3
GCAATGT	20	0.006917726	52.5	32
ACAAGGG	20	0.006917726	52.5	3
CACTTTC	20	0.006917726	52.5	9
ATCGTAT	30	5.83136E-4	46.666668	39
GTAGATA	30	5.83136E-4	46.666668	33
GTCACTG	30	5.83136E-4	46.666668	70
CTTTACA	40	4.538781E-5	43.75	1
CTATACA	50	3.421741E-6	42.000004	1
CCGTCTT	670	0.0	37.611942	47
CGTCTTC	680	0.0	37.57353	48
GCCGTCT	690	0.0	37.536232	46
TATGCCG	695	0.0	37.26619	43
CTGCTTG	690	0.0	37.028984	54
TGCCGTC	690	0.0	37.028984	45