FastQCFastQC Report
Wed 25 May 2016
SRR1779739_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779739_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23699
Sequences flagged as poor quality0
Sequence length76
%GC54

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGTGG550.070.0000145
CGGTGTT252.7558508E-670.0000146
CGGTGGT500.070.0000146
GGTGTTC252.7558508E-670.0000147
ATCCCCA150.002197984870.04
GTGTCTA150.002197984870.036
GGCAACC150.002197984870.028
TATGTAT150.002197984870.027
TGTCACT150.002197984870.053
CCCACGA150.002197984870.021
ATTACGT150.002197984870.012
AACTTAT150.002197984870.056
TTCCTAC150.002197984870.019
ACTTATT207.769413E-570.057
AGTGTAG353.5088306E-970.035
CGGCTGT150.002197984870.031
GACTCTG150.002197984870.014
GTTATAC150.002197984870.01
CTTCCGA309.8156306E-870.018
TTACGCT353.5088306E-970.013
Produced by FastQC (version 0.11.5)