##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779739_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	23699
Sequences flagged as poor quality	0
Sequence length	76
%GC	54
>>END_MODULE
>>Per base sequence quality	warn
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	22.967087218869995	23.0	12.0	32.0	12.0	34.0
2	23.629478037047978	23.0	12.0	32.0	12.0	34.0
3	23.304865184184987	23.0	11.0	32.0	11.0	34.0
4	22.89476349213047	23.0	12.0	32.0	11.0	34.0
5	23.47710873876535	23.0	11.0	33.0	11.0	34.0
6	24.165239039621923	26.0	12.0	34.0	11.0	38.0
7	25.023081142664246	27.0	12.0	36.0	12.0	38.0
8	24.383560487784294	26.0	11.0	35.0	11.0	38.0
9	25.922190809738808	27.0	12.0	37.0	12.0	38.0
10-11	24.901641419469176	26.0	11.0	36.0	11.0	38.0
12-13	25.42318241276003	27.0	11.0	35.5	11.0	38.0
14-15	24.13422507278788	26.0	11.0	34.5	11.0	38.0
16-17	24.34457150090721	26.0	11.0	35.0	11.0	38.0
18-19	24.750854466433182	26.0	11.5	34.5	11.0	38.0
20-21	23.858896999873412	24.5	11.0	34.0	11.0	37.5
22-23	24.172412338073336	24.5	11.0	35.0	11.0	37.5
24-25	24.056584666019663	25.0	11.0	35.0	11.0	38.0
26-27	23.319633739820247	23.0	11.0	34.0	10.0	38.0
28-29	24.019663276931517	25.0	11.0	35.5	11.0	38.0
30-31	24.433836870754043	26.0	11.0	36.5	11.0	38.0
32-33	24.581817798219333	26.0	11.0	37.0	11.0	38.0
34-35	24.65694755052956	26.0	11.0	37.0	11.0	38.0
36-37	24.06675387147137	25.0	11.0	34.0	11.0	38.0
38-39	23.941200050635047	24.5	11.0	34.0	11.0	37.0
40-41	23.225178277564453	23.5	11.0	34.0	11.0	37.0
42-43	23.71000886113338	25.5	11.0	34.0	11.0	38.0
44-45	24.638951010591164	26.0	11.0	36.5	11.0	38.0
46-47	24.21038862399257	25.0	11.0	35.5	11.0	38.0
48-49	23.338664078653107	23.5	11.0	34.0	11.0	38.0
50-51	22.640702139330774	23.0	11.0	33.0	11.0	37.0
52-53	23.44309886493101	24.0	11.0	35.0	11.0	37.5
54-55	23.310456137389764	23.5	11.0	34.0	11.0	38.0
56-57	23.705472804759694	24.0	11.0	35.0	11.0	38.0
58-59	23.925819654837756	24.5	11.0	36.0	11.0	38.0
60-61	24.525887168234945	25.5	11.0	37.0	11.0	38.0
62-63	24.537723110679778	25.5	11.0	37.0	11.0	38.0
64-65	24.374214101860836	25.5	11.0	36.5	11.0	38.0
66-67	23.82811511034221	24.5	11.0	34.0	11.0	38.0
68-69	23.48472509388582	24.0	11.0	34.0	11.0	38.0
70-71	23.268597831132116	24.0	11.0	34.0	11.0	38.0
72-73	23.056500274273176	24.0	11.0	34.0	11.0	37.0
74-75	23.048968310899195	23.0	11.0	34.0	11.0	37.5
76	22.622178150976833	23.0	11.0	34.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	fail
#Quality	Count
4	1.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	3.0
12	28.0
13	149.0
14	445.0
15	754.0
16	1196.0
17	1878.0
18	2396.0
19	2498.0
20	2114.0
21	1614.0
22	1186.0
23	767.0
24	565.0
25	410.0
26	400.0
27	364.0
28	395.0
29	421.0
30	450.0
31	557.0
32	628.0
33	761.0
34	984.0
35	1184.0
36	1075.0
37	476.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.460989915186293	15.042828811342252	14.211570108443395	45.28461116502806
2	13.582851597113802	11.65450018988143	52.25958901219461	22.50305920081016
3	15.650449386050044	11.14393012363391	49.81644795139035	23.389172538925692
4	13.35077429427402	23.2836828558167	26.612937254736487	36.752605595172795
5	13.131355753407318	17.92480695387991	47.55474914553356	21.389088147179205
6	21.152791257015064	35.57112114435208	15.57871640153593	27.697371197096928
7	19.439638803325035	19.802523313219965	18.47335330604667	42.284484577408335
8	16.271415309308804	33.73280445607224	15.967592201873575	34.028188032745376
9	16.803105747320448	14.866233437420878	16.959237066419107	51.37142374883956
10-11	19.03747151658368	21.147354207106083	28.74715165836779	31.068022617942443
12-13	17.16564339515159	14.070510791822265	35.1288056206089	33.63504019241724
14-15	24.423486718568686	20.581471401143535	22.104774563790958	32.890267316496825
16-17	30.669254789433708	14.8493543758967	21.28449658199004	33.19689425267955
18-19	18.746307705291585	14.00751118237826	31.16296733901595	36.083213773314206
20-21	30.108025993754744	14.100346020761245	22.48501983289729	33.30660815258671
22-23	32.291754578445435	12.536922947084141	21.225419866655415	33.945902607815
24-25	20.287775855521332	21.682349466222202	19.11262078568716	38.917253892569306
26-27	28.726052831462574	21.261287872394295	21.57988015866318	28.432779137479958
28-29	22.50986349347005	19.925311728590415	23.106947697111632	34.4578770808279
30-31	20.79922356316989	19.666216558359356	25.519031141868513	34.01552873660225
32-33	22.767744113427295	18.265254451852478	25.293273693982616	33.67372774073761
34-35	21.567220862520045	19.611359608405774	24.801671027090894	34.01974850198329
36-37	29.517258840408473	13.123470335049372	31.200945227445352	26.1583255970968
38-39	29.266182800236308	10.6591273525192	37.13393535319437	22.940754494050132
40-41	24.170816102624695	22.839480124905055	22.850029538357667	30.139674234112583
42-43	16.644513365824842	23.860159925733697	27.489081587442243	32.006245120999225
44-45	17.48844863598962	15.334303858894021	32.93246408000506	34.244783425111294
46-47	28.57142857142857	14.114817393505918	25.77167331265692	31.54208072240859
48-49	27.832985210034387	14.836382049496805	29.80568390404456	27.524948836424244
50-51	40.914845134610516	12.408220102962275	23.62224660308887	23.05468815933834
52-53	16.990885306776942	18.132331842349565	30.416068866571017	34.46071398430247
54-55	27.352519199932484	15.315638450502153	24.72149548485104	32.61034686471432
56-57	26.843471105766188	16.211996539865392	23.282064265670822	33.662468088697594
58-59	17.929783104059414	19.70630432947928	28.896953329394886	33.46695923706642
60-61	17.328466537260528	16.976116127943286	28.643767406532195	37.05164992826399
62-63	17.292598531521648	20.149379694488985	27.945396235969277	34.61262553802008
64-65	17.298928179593215	22.092581652460122	26.844037471516586	33.764452696430084
66-67	15.229133260190734	32.61034686471432	18.256814921090385	33.90370495400456
68-69	15.887219314536551	31.377680229613368	18.204457200742866	34.53064325510721
70-71	15.929614313444173	32.903620558696936	16.786226685796272	34.38053844206262
72-73	14.47168537429319	36.78791459194869	15.640560384842603	33.09983964891552
74-75	16.957127183728584	24.9810110557853	19.860325765887417	38.2015359945987
76	18.048698147444824	21.551251213233744	23.04089125205722	37.359159387264214
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.5
17	1.0
18	1.0
19	1.0
20	2.5
21	4.0
22	3.5
23	6.5
24	11.5
25	13.0
26	17.0
27	27.5
28	46.0
29	58.0
30	75.5
31	109.5
32	138.5
33	151.0
34	198.5
35	266.0
36	323.5
37	361.0
38	401.5
39	505.0
40	597.0
41	681.5
42	737.0
43	811.0
44	910.5
45	953.0
46	970.0
47	990.5
48	1013.5
49	1009.0
50	1002.0
51	987.5
52	962.0
53	915.0
54	879.0
55	852.0
56	843.5
57	819.0
58	776.0
59	763.0
60	746.0
61	742.5
62	743.0
63	736.0
64	701.0
65	680.5
66	675.5
67	663.0
68	666.5
69	638.0
70	587.5
71	569.0
72	505.0
73	413.5
74	348.0
75	310.0
76	293.0
77	233.5
78	173.0
79	155.0
80	126.0
81	79.0
82	50.5
83	40.0
84	32.0
85	17.0
86	7.5
87	5.0
88	2.5
89	0.5
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.5
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0042195873243596775
9	0.0042195873243596775
10-11	0.0042195873243596775
12-13	0.0021097936621798387
14-15	0.0021097936621798387
16-17	0.0042195873243596775
18-19	0.0042195873243596775
20-21	0.0042195873243596775
22-23	0.0042195873243596775
24-25	0.0
26-27	0.0042195873243596775
28-29	0.0021097936621798387
30-31	0.0042195873243596775
32-33	0.0042195873243596775
34-35	0.0042195873243596775
36-37	0.0042195873243596775
38-39	0.0042195873243596775
40-41	0.0042195873243596775
42-43	0.0021097936621798387
44-45	0.0021097936621798387
46-47	0.0021097936621798387
48-49	0.0021097936621798387
50-51	0.0042195873243596775
52-53	0.0042195873243596775
54-55	0.0042195873243596775
56-57	0.0021097936621798387
58-59	0.0042195873243596775
60-61	0.0042195873243596775
62-63	0.0042195873243596775
64-65	0.0042195873243596775
66-67	0.0042195873243596775
68-69	0.029537111270517745
70-71	0.0042195873243596775
72-73	0.0042195873243596775
74-75	0.0042195873243596775
76	0.008439174648719355
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	23699.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	99.68775053799739
#Duplication Level	Percentage of deduplicated	Percentage of total
1	99.6952380952381	99.38394025064349
2	0.2962962962962963	0.5907422254103549
3	0.008465608465608466	0.025317523946158067
4	0.0	0.0
5	0.0	0.0
6	0.0	0.0
7	0.0	0.0
8	0.0	0.0
9	0.0	0.0
>10	0.0	0.0
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0042195873243596775	0.0
5	0.0	0.0	0.0	0.012658761973079033	0.0
6	0.0	0.0	0.0	0.012658761973079033	0.0
7	0.0	0.0	0.0	0.01687834929743871	0.0
8	0.0	0.0	0.0	0.01687834929743871	0.0
9	0.0	0.0	0.0	0.029537111270517745	0.0
10	0.0	0.0	0.0	0.042195873243596776	0.0
11	0.0	0.0	0.0	0.042195873243596776	0.0
12	0.0	0.0	0.0	0.04641546056795645	0.0
13	0.0	0.0	0.0	0.04641546056795645	0.0
14	0.0	0.0	0.0	0.05485463521667581	0.0
15	0.0	0.0	0.0	0.05485463521667581	0.0
16	0.0	0.0	0.0	0.05485463521667581	0.0
17	0.0	0.0	0.0	0.05907422254103549	0.0
18	0.0	0.0	0.0	0.05907422254103549	0.0
19	0.0	0.0	0.0	0.06751339718975484	0.0
20	0.0	0.0	0.0	0.06751339718975484	0.0
21	0.0	0.0	0.0	0.06751339718975484	0.0
22	0.0	0.0	0.0	0.10548968310899194	0.0
23	0.0	0.0	0.0	0.10970927043335162	0.0
24	0.0	0.0	0.0	0.12236803240643065	0.0
25	0.0	0.0	0.0	0.12236803240643065	0.0
26	0.0	0.0	0.0	0.13502679437950968	0.0
27	0.0	0.0	0.0	0.16034431832566776	0.0
28	0.0	0.0	0.0	0.1856618422718258	0.0
29	0.0	0.0	0.0	0.21941854086670323	0.0
30	0.0	0.0	0.0	0.24051647748850163	0.0
31	0.0	0.0	0.0	0.24895565213722098	0.0
32	0.0	0.0	0.0	0.2827123507320984	0.0
33	0.0	0.0	0.0	0.3206886366513355	0.0
34	0.0	0.0	0.0	0.3755432718680113	0.0
35	0.0	0.0	0.0	0.4683741930039242	0.0
36	0.0	0.0	0.0	0.5485463521667581	0.0
37	0.0	0.0	0.0	0.7299886071142242	0.0
38	0.0	0.0	0.0	0.8776741634668129	0.0
39	0.0	0.0	0.0	1.0759947677117179	0.0
40	0.0	0.0	0.0	1.2996328959027807	0.0
41	0.0	0.0	0.0	1.4895143254989662	0.0
42	0.0	0.0	0.0	1.6540782311489937	0.0
43	0.0	0.0	0.0	1.9325709945567324	0.0
44	0.0	0.0	0.0	2.1013544875311196	0.0
45	0.0	0.0	0.0	2.206844170640111	0.0
46	0.0	0.0	0.0	2.367188488965779	0.0
47	0.0	0.0	0.0	2.569728680535044	0.0
48	0.0	0.0	0.0	2.721633824211992	0.0
49	0.0	0.0	0.0	2.8060255706991857	0.0
50	0.0	0.0	0.0	2.9663698890248535	0.0
51	0.0	0.0	0.0	3.1478121439723195	0.0
52	0.0	0.0	0.0	3.3165956369467064	0.0
53	0.0	0.0	0.0	3.5275750031646904	0.0
54	0.0	0.0	0.0	3.7469935440313935	0.0
55	0.0	0.0	0.0	3.911557449681421	0.0
56	0.0	0.0	0.0	4.0508038313852905	0.0
57	0.0	0.0	0.0	4.19005021308916	0.0
58	0.0	0.0	0.0	4.2828811342250726	0.0
59	0.0	0.0	0.0	4.413688341280222	0.0
60	0.0	0.0	0.0	4.4938605004430565	0.0
61	0.0	0.0	0.0	4.624667707498206	0.0
62	0.0	0.0	0.0	4.683741930039242	0.0
63	0.0	0.0	0.0	4.776572851175155	0.0
64	0.0	0.0	0.0	4.84830583568927	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGGTGG	55	0.0	70.00001	45
CGGTGTT	25	2.7558508E-6	70.00001	46
CGGTGGT	50	0.0	70.00001	46
GGTGTTC	25	2.7558508E-6	70.00001	47
ATCCCCA	15	0.0021979848	70.0	4
GTGTCTA	15	0.0021979848	70.0	36
GGCAACC	15	0.0021979848	70.0	28
TATGTAT	15	0.0021979848	70.0	27
TGTCACT	15	0.0021979848	70.0	53
CCCACGA	15	0.0021979848	70.0	21
ATTACGT	15	0.0021979848	70.0	12
AACTTAT	15	0.0021979848	70.0	56
TTCCTAC	15	0.0021979848	70.0	19
ACTTATT	20	7.769413E-5	70.0	57
AGTGTAG	35	3.5088306E-9	70.0	35
CGGCTGT	15	0.0021979848	70.0	31
GACTCTG	15	0.0021979848	70.0	14
GTTATAC	15	0.0021979848	70.0	1
CTTCCGA	30	9.8156306E-8	70.0	18
TTACGCT	35	3.5088306E-9	70.0	13
>>END_MODULE