FastQCFastQC Report
Wed 25 May 2016
SRR1779739_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779739_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23699
Sequences flagged as poor quality0
Sequence length76
%GC49

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT904838.17882611080636No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG11614.898940883581586No Hit
ATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTCTT7753.27018017637875Illumina PCR Primer Index 9 (95% over 22bp)
CTCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC6412.7047554749145535No Hit
CTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTC4111.7342503903118274No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC3961.6709565804464324No Hit
CTTTACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTC3381.426220515633571No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGACCTCGTATGCCGT2511.0591164184142792No Hit
CTTAATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG1550.65403603527575No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC1470.6202793366808727No Hit
TTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTC1370.5780834634372759No Hit
TATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTCT1200.5063504789231613Illumina PCR Primer Index 9 (95% over 21bp)
CTTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG1170.4936917169500823No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGAACTCGTATGCCGT1160.48947212962572256No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGAGCTCGTATGCCGT1000.42195873243596776No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTTAACAGACCTCGTATGCCG850.35866492257057264No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATATCGTATGCCGT840.3544453352462129No Hit
TCTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT690.29115152538081773No Hit
CCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG430.18144225494746613No Hit
ATACACATCTCCGAGCCCACGAGACGTTAACAGACCTCGTATGCCGTCTT330.13924638170386938No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTTAACAGACCTCGTATGCC300.12658761973079033No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATTTCGTATGCCGT280.11814844508207098No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTTAACAGAACTCGTATGCCG270.11392885775771129No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGC270.11392885775771129No Hit
CTCTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG260.10970927043335163No Hit
CTTATTACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG240.10127009578463227No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGGCGT150.002197984870.029
CTTTACA401.2551027E-1070.01
GGCGTAC150.002197984870.031
CGAGACA150.002197984870.023
CCGATCT150.002197984870.034
TCTTAAT309.8156306E-870.01
GAGACAT150.002197984870.024
TCTTTAT501.2732926E-1163.0000041
TTACACA453.583409E-1062.2222253
TTTACAC453.583409E-1062.2222252
CTATACA1000.056.0000041
CTTATAC13800.053.0072481
AGGCGTA200.006868564552.530
GGAGGCG200.006868564552.528
TTATACA14700.049.76192
TATACAC15750.046.6666683
CTTAATA451.6163358E-646.6666682
TAATACA451.6163358E-646.6666684
TTAATAC451.6163358E-646.6666683
AACAACA305.7627464E-446.66666470
Produced by FastQC (version 0.11.5)