FastQCFastQC Report
Wed 25 May 2016
SRR1779738_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779738_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences93049
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTGTC150.00221846370.034
AGTACGG150.00221846370.05
TAGATCT553.45608E-1157.27272439
GATCTTG252.3795883E-456.00000441
GCCGTAT252.3795883E-456.00000454
CGTGCGA200.006932064452.510
CCGTGTA200.006932064452.534
GTAGATC407.409544E-752.538
CAAGGTC200.006932064452.564
CGGTGGT407.409544E-752.546
GACGCTT200.006932064452.514
CGTCCTA200.006932064452.515
CTTACGC407.409544E-752.512
TGCAACG200.006932064452.530
AGATCTC451.6690119E-646.66666840
CGGCGGT305.851422E-446.66666446
AGATCTT305.851422E-446.66666440
AGGACAA305.851422E-446.66666450
GTGTCGA305.851422E-446.66666436
TCTTACG404.5621517E-543.7511
Produced by FastQC (version 0.11.5)