##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779738_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	93049
Sequences flagged as poor quality	0
Sequence length	76
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.725563950176788	34.0	32.0	34.0	21.0	34.0
2	28.742286322260316	34.0	23.0	34.0	12.0	34.0
3	27.69251684596288	33.0	23.0	34.0	11.0	34.0
4	30.538544207890467	34.0	32.0	34.0	21.0	34.0
5	29.86963857752367	34.0	31.0	34.0	11.0	34.0
6	32.60836763425722	37.0	32.0	38.0	12.0	38.0
7	33.740179905211235	37.0	34.0	38.0	21.0	38.0
8	32.94594246042408	37.0	34.0	38.0	12.0	38.0
9	34.386914421433865	38.0	34.0	38.0	25.0	38.0
10-11	34.22797665746005	38.0	35.0	38.0	23.0	38.0
12-13	33.007866822856776	37.5	31.5	38.0	18.5	38.0
14-15	33.26108287031564	37.0	33.0	38.0	21.0	38.0
16-17	32.848843082676865	37.5	31.0	38.0	18.5	38.0
18-19	33.3735881094907	37.0	33.5	38.0	22.0	38.0
20-21	32.274178121204955	36.5	30.0	38.0	17.0	38.0
22-23	32.26914313963611	37.0	29.0	38.0	17.0	38.0
24-25	33.72386054659373	37.0	34.0	38.0	22.0	38.0
26-27	32.04550290707047	37.0	30.0	38.0	16.0	38.0
28-29	34.453325667121625	37.5	35.0	38.0	24.5	38.0
30-31	34.88862319852981	38.0	36.5	38.0	26.0	38.0
32-33	34.97452954894733	38.0	37.0	38.0	26.0	38.0
34-35	35.034288385689266	38.0	37.0	38.0	26.5	38.0
36-37	32.647067674021216	37.0	30.5	38.0	16.5	38.0
38-39	30.621269438682845	36.0	26.0	38.0	11.0	38.0
40-41	32.20964760502531	36.5	30.0	38.0	16.0	38.0
42-43	33.31667186106245	37.0	32.5	38.0	18.5	38.0
44-45	35.02685681737579	38.0	36.5	38.0	27.0	38.0
46-47	33.426216294640454	37.5	32.0	38.0	19.0	38.0
48-49	32.81357134413051	37.0	30.5	38.0	17.5	38.0
50-51	30.433803694827457	36.5	24.5	38.0	11.0	38.0
52-53	34.220211931348004	37.5	35.0	38.0	24.5	38.0
54-55	33.1910659974852	37.5	31.0	38.0	18.0	38.0
56-57	33.640270180227624	37.5	33.5	38.0	18.5	38.0
58-59	34.84993927930445	38.0	36.5	38.0	26.0	38.0
60-61	35.249164418747114	38.0	37.0	38.0	27.0	38.0
62-63	35.14410149491128	38.0	37.0	38.0	26.5	38.0
64-65	34.82706960848585	38.0	36.5	38.0	25.0	38.0
66-67	32.64697632430225	37.5	32.0	38.0	11.0	38.0
68-69	32.728589237928404	37.0	32.5	38.0	16.0	38.0
70-71	32.14886242732324	37.0	30.0	38.0	11.0	38.0
72-73	30.946275618222657	36.5	26.0	38.0	11.0	38.0
74-75	33.328789132607554	37.0	32.5	38.0	18.0	38.0
76	33.964212404217136	37.0	34.0	38.0	23.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	13.0
13	84.0
14	270.0
15	418.0
16	690.0
17	990.0
18	1322.0
19	1220.0
20	1108.0
21	916.0
22	791.0
23	692.0
24	696.0
25	779.0
26	901.0
27	1164.0
28	1478.0
29	1895.0
30	2703.0
31	3685.0
32	5387.0
33	7846.0
34	11795.0
35	16491.0
36	19304.0
37	10409.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	39.723156616406406	19.4961794323421	11.160786252404648	29.619877698846842
2	22.02925340412041	17.92281486098722	36.9858891551763	23.062042579716064
3	20.494578125503768	19.023310298874787	33.976721942202495	26.50538963341895
4	14.082902556717428	18.03458392889768	34.34641962836785	33.53609388601704
5	14.527829423207129	30.976152349837182	38.17128609657277	16.324732130382916
6	28.263603047856506	33.168545605003814	17.521950800116066	21.04590054702361
7	28.242108996335265	28.008898537329795	20.498876935808017	23.250115530526926
8	23.610140893507722	32.11103827015873	18.820191511999056	25.458629324334492
9	24.11202699652871	16.115165128050812	20.664381132521576	39.10842674289891
10-11	24.98629754215521	24.340401293941902	27.054562649786675	23.61873851411622
12-13	25.145353523412396	20.914786832744035	28.378596223495144	25.561263420348418
14-15	23.087422150813822	23.357173946920152	24.197058523243257	29.358345379022765
16-17	23.563107213481214	25.051586278050035	26.815729515948757	24.56957699251999
18-19	22.045492404498727	24.900455138986658	27.87148637538488	25.182566081129732
20-21	23.050758202667414	24.31836989113263	28.125503766832527	24.50536813936743
22-23	23.3812292448065	23.735881094906986	28.71067931949833	24.172210340788187
24-25	22.202817870154433	25.963202183795637	25.689690378187834	26.144289567862096
26-27	21.973906221453213	24.952444411009253	28.538189556040365	24.53545981149717
28-29	22.482240539930572	26.245311610011928	26.82726305494954	24.445184795107956
30-31	22.337813075976506	25.81986813328533	27.010107632041354	24.83221115869681
32-33	22.230222785844017	26.18835237348064	26.547840385173405	25.033584455501938
34-35	22.585411987232533	25.4102677084117	27.094863996389	24.90945630796677
36-37	22.856237036400177	23.891712968435986	30.219024385001454	23.03302561016239
38-39	22.777783748347645	23.063117282292126	32.48503476662833	21.674064202731895
40-41	22.540395600144013	23.910648747696094	26.275007119942828	27.27394853221707
42-43	21.06040903180045	24.45861857730873	27.05832410880289	27.42264828208793
44-45	21.50157443927393	24.561790024610687	28.356564820685875	25.580070715429503
46-47	22.38175584906877	23.509118851357886	29.5913980805812	24.517727218992142
48-49	22.3043772635923	24.11202699652871	29.65964169416114	23.923954045717846
50-51	22.81647304108588	22.520392481380778	31.996045094520092	22.66708938301325
52-53	20.571419359692207	24.86646820492429	28.8745714623478	25.68754097303571
54-55	21.476856280024503	24.05882921901364	29.09327343657643	25.37104106438543
56-57	21.065782544680758	24.5515803501381	28.78160968951843	25.601027415662713
58-59	20.024503350403283	25.237376207031815	28.575957699479304	26.16216274308559
60-61	20.25513713352248	24.725410540796148	28.466490413549995	26.552961912131373
62-63	19.832130555570483	25.271229520088983	28.22452806869536	26.672111855645177
64-65	20.62569183978334	25.363518146352998	27.344194994035405	26.666595019828264
66-67	19.540884592443724	23.906349914292008	25.47005056502791	31.08271492823635
68-69	19.688606792177012	24.368103962551125	25.526826358247295	30.416462887024565
70-71	20.347990564060677	23.916559643626712	24.734950052929385	31.000499739383226
72-73	19.825038420617094	23.13727176004041	23.465056045739342	33.57263377360316
74-75	21.04590054702361	25.038957968382253	25.066900235359864	28.848241249234274
76	22.23046169718855	24.388487662281833	25.213868111082455	28.167182529447167
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.5
10	1.0
11	0.5
12	0.0
13	0.0
14	0.0
15	1.0
16	2.0
17	2.0
18	2.5
19	9.0
20	17.0
21	19.0
22	29.5
23	63.5
24	114.0
25	141.0
26	182.5
27	278.0
28	421.5
29	511.0
30	623.0
31	833.0
32	1081.0
33	1231.0
34	1484.0
35	1950.5
36	2481.0
37	2798.0
38	3167.0
39	3864.0
40	4545.0
41	5102.0
42	5306.0
43	5450.0
44	5702.0
45	5845.5
46	5881.0
47	5753.5
48	5478.5
49	5101.5
50	4872.0
51	4599.5
52	4004.5
53	3350.5
54	3019.0
55	2764.5
56	2249.0
57	1847.5
58	1707.0
59	1576.0
60	1336.5
61	1210.0
62	1192.0
63	1123.5
64	1035.5
65	1031.0
66	1004.5
67	963.0
68	964.0
69	911.5
70	837.0
71	816.0
72	765.0
73	688.5
74	597.5
75	532.0
76	460.0
77	344.5
78	256.5
79	212.0
80	178.0
81	121.0
82	81.0
83	64.0
84	52.5
85	31.5
86	16.0
87	10.0
88	11.5
89	8.5
90	3.5
91	2.0
92	1.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.5
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	5.373512880310374E-4
16-17	0.0010747025760620749
18-19	5.373512880310374E-4
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	5.373512880310374E-4
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	5.373512880310374E-4
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	5.373512880310374E-4
60-61	0.0010747025760620749
62-63	5.373512880310374E-4
64-65	0.0
66-67	5.373512880310374E-4
68-69	0.01665788992896216
70-71	5.373512880310374E-4
72-73	0.0
74-75	0.0
76	0.0010747025760620749
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	93049.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	94.93492675901945
#Duplication Level	Percentage of deduplicated	Percentage of total
1	96.3933164281833	91.51092435168567
2	2.658032965042565	5.046803297187504
3	0.5433798224959246	1.5475717095293877
4	0.22754030067016845	0.8640608711539082
5	0.08829922115558776	0.4191340046642092
6	0.046413693171526896	0.2643768337112704
7	0.015848578156131138	0.10532085245408332
8	0.012452454265531607	0.09457382669346259
9	0.0022640825937330195	0.019344646369117348
>10	0.012452454265531607	0.12788960655138693
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0010747025760620749	0.0
6	0.0	0.0	0.0	0.0010747025760620749	0.0
7	0.0	0.0	0.0	0.0010747025760620749	0.0
8	0.0	0.0	0.0	0.0010747025760620749	0.0
9	0.0	0.0	0.0	0.0010747025760620749	0.0
10	0.0	0.0	0.0	0.0010747025760620749	0.0
11	0.0	0.0	0.0	0.0010747025760620749	0.0
12	0.0	0.0	0.0010747025760620749	0.0010747025760620749	0.0
13	0.0	0.0	0.0010747025760620749	0.0021494051521241497	0.0
14	0.0	0.0	0.0010747025760620749	0.0021494051521241497	0.0
15	0.0	0.0	0.0010747025760620749	0.0021494051521241497	0.0
16	0.0	0.0	0.0010747025760620749	0.0021494051521241497	0.0
17	0.0	0.0	0.0010747025760620749	0.0021494051521241497	0.0
18	0.0	0.0	0.0010747025760620749	0.0021494051521241497	0.0
19	0.0	0.0	0.0010747025760620749	0.0032241077281862244	0.0
20	0.0	0.0	0.0010747025760620749	0.004298810304248299	0.0
21	0.0	0.0	0.0010747025760620749	0.008597620608496599	0.0
22	0.0	0.0	0.0010747025760620749	0.013971133488806972	0.0
23	0.0	0.0	0.0010747025760620749	0.013971133488806972	0.0
24	0.0010747025760620749	0.0	0.0010747025760620749	0.019344646369117348	0.0
25	0.0010747025760620749	0.0	0.0010747025760620749	0.02256875409730357	0.0
26	0.0010747025760620749	0.0	0.0010747025760620749	0.025792861825489795	0.0
27	0.0010747025760620749	0.0	0.0010747025760620749	0.03546518501004847	0.0
28	0.0010747025760620749	0.0	0.0010747025760620749	0.04513750819460714	0.0
29	0.0010747025760620749	0.0	0.0010747025760620749	0.05480983137916581	0.0
30	0.0010747025760620749	0.0	0.0010747025760620749	0.07307977517222108	0.0
31	0.0010747025760620749	0.0	0.0010747025760620749	0.08275209835677975	0.0
32	0.0010747025760620749	0.0	0.0010747025760620749	0.09564852926952466	0.0
33	0.0010747025760620749	0.0	0.0010747025760620749	0.11606787821470409	0.0
34	0.0010747025760620749	0.0010747025760620749	0.0010747025760620749	0.13863663231200765	0.0
35	0.0010747025760620749	0.0010747025760620749	0.0010747025760620749	0.18162473535449064	0.0
36	0.0010747025760620749	0.0010747025760620749	0.0010747025760620749	0.23428516158153231	0.0
37	0.0010747025760620749	0.0010747025760620749	0.0010747025760620749	0.38904233253447107	0.0
38	0.0010747025760620749	0.0010747025760620749	0.0010747025760620749	0.6115057657793206	0.0
39	0.0010747025760620749	0.0010747025760620749	0.0010747025760620749	0.8210727681114252	0.0
40	0.0010747025760620749	0.0010747025760620749	0.0010747025760620749	1.1316618125933648	0.0
41	0.0010747025760620749	0.0010747025760620749	0.0010747025760620749	1.6034562434846156	0.0010747025760620749
42	0.0010747025760620749	0.0010747025760620749	0.0010747025760620749	2.069877161495556	0.0010747025760620749
43	0.0010747025760620749	0.0010747025760620749	0.0010747025760620749	2.6179754752872144	0.0010747025760620749
44	0.0010747025760620749	0.0010747025760620749	0.0010747025760620749	3.113413362851831	0.0010747025760620749
45	0.0010747025760620749	0.0010747025760620749	0.0010747025760620749	3.4949327773538674	0.0010747025760620749
46	0.0010747025760620749	0.0010747025760620749	0.0010747025760620749	3.9312620232350697	0.0010747025760620749
47	0.0010747025760620749	0.0010747025760620749	0.0010747025760620749	4.515900224612839	0.0010747025760620749
48	0.0010747025760620749	0.0010747025760620749	0.0010747025760620749	4.918913690636116	0.0010747025760620749
49	0.0010747025760620749	0.0010747025760620749	0.0010747025760620749	5.315478941203022	0.0010747025760620749
50	0.0010747025760620749	0.0010747025760620749	0.0010747025760620749	5.892594224548357	0.0010747025760620749
51	0.0010747025760620749	0.0010747025760620749	0.0010747025760620749	6.601897924749325	0.0010747025760620749
52	0.0010747025760620749	0.0010747025760620749	0.0010747025760620749	7.17363969521435	0.0010747025760620749
53	0.0010747025760620749	0.0010747025760620749	0.0010747025760620749	7.938827929370547	0.0010747025760620749
54	0.0010747025760620749	0.0010747025760620749	0.0010747025760620749	8.669625681092757	0.0010747025760620749
55	0.0010747025760620749	0.0010747025760620749	0.0010747025760620749	9.125299573343078	0.0010747025760620749
56	0.0010747025760620749	0.0010747025760620749	0.0010747025760620749	9.605691624842825	0.0010747025760620749
57	0.0010747025760620749	0.0010747025760620749	0.0010747025760620749	10.01300390117035	0.0010747025760620749
58	0.0010747025760620749	0.0010747025760620749	0.0010747025760620749	10.392373910520263	0.0010747025760620749
59	0.0010747025760620749	0.0010747025760620749	0.0010747025760620749	10.842674289890272	0.0010747025760620749
60	0.0010747025760620749	0.0010747025760620749	0.0010747025760620749	11.160786252404646	0.0010747025760620749
61	0.0010747025760620749	0.0010747025760620749	0.0010747025760620749	11.481047620071145	0.0010747025760620749
62	0.0010747025760620749	0.0010747025760620749	0.0010747025760620749	11.729303915141484	0.0010747025760620749
63	0.0010747025760620749	0.0010747025760620749	0.0010747025760620749	12.073208739481348	0.0010747025760620749
64	0.0010747025760620749	0.0010747025760620749	0.0010747025760620749	12.375200163354792	0.0010747025760620749
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGTGTC	15	0.002218463	70.0	34
AGTACGG	15	0.002218463	70.0	5
TAGATCT	55	3.45608E-11	57.272724	39
GATCTTG	25	2.3795883E-4	56.000004	41
GCCGTAT	25	2.3795883E-4	56.000004	54
CGTGCGA	20	0.0069320644	52.5	10
CCGTGTA	20	0.0069320644	52.5	34
GTAGATC	40	7.409544E-7	52.5	38
CAAGGTC	20	0.0069320644	52.5	64
CGGTGGT	40	7.409544E-7	52.5	46
GACGCTT	20	0.0069320644	52.5	14
CGTCCTA	20	0.0069320644	52.5	15
CTTACGC	40	7.409544E-7	52.5	12
TGCAACG	20	0.0069320644	52.5	30
AGATCTC	45	1.6690119E-6	46.666668	40
CGGCGGT	30	5.851422E-4	46.666664	46
AGATCTT	30	5.851422E-4	46.666664	40
AGGACAA	30	5.851422E-4	46.666664	50
GTGTCGA	30	5.851422E-4	46.666664	36
TCTTACG	40	4.5621517E-5	43.75	11
>>END_MODULE