FastQCFastQC Report
Wed 25 May 2016
SRR1779738_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779738_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences93049
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT32693.513202721146923No Hit
ATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTCTT5690.6115057657793206RNA PCR Primer, Index 42 (95% over 22bp)
TCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG4810.516931939085858No Hit
CTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTC2430.2611527259830842RNA PCR Primer, Index 32 (95% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC2300.2471815924942772No Hit
TCTTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC2250.24180807961396686No Hit
CTTTACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTC2210.23750926930971852No Hit
CTTATACACATCTCCGAGCCCACGAGACATCACTCGACCTCGTATGCCGT1220.13111371427957313No Hit
CTTAATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG970.10424614987802126No Hit
TATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTCT940.10102204214983504RNA PCR Primer, Index 42 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCCAT150.00221846370.050
AGTTCAT150.00221846370.012
TTCGACG150.00221846370.010
CCTCGTA200.006932064452.538
TATAGGG200.006932064452.52
TAGCTCA200.006932064452.531
AATGCCG350.001249827940.040
CTTAATA459.136822E-538.888892
GCCTATG459.136822E-538.888891
CTTTATA459.136822E-538.888892
GGCTATG501.6984473E-435.0000041
CTATACA601.204589E-535.01
TCTAGCA400.002408056835.03
CATACCT400.002408056835.041
TATGCCG7450.034.295343
GTATGCC7450.034.295342
TCGTATG7450.034.295340
CTCGTAT7350.034.28571739
CGTCTTC7350.034.28571748
CCGTCTT7350.034.28571747
Produced by FastQC (version 0.11.5)