##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779738_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	93049
Sequences flagged as poor quality	0
Sequence length	76
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.374781029350125	34.0	34.0	34.0	33.0	34.0
2	33.383873013143614	34.0	34.0	34.0	33.0	34.0
3	33.435888617825015	34.0	34.0	34.0	34.0	34.0
4	33.4868725080334	34.0	34.0	34.0	34.0	34.0
5	33.46416404260121	34.0	34.0	34.0	34.0	34.0
6	36.846919365065716	38.0	37.0	38.0	34.0	38.0
7	36.8857591161646	38.0	37.0	38.0	35.0	38.0
8	36.616460144654965	38.0	37.0	38.0	34.0	38.0
9	36.639910154864644	38.0	37.0	38.0	34.0	38.0
10-11	36.524852497071436	38.0	37.0	38.0	34.0	38.0
12-13	36.51724360283292	38.0	37.0	38.0	34.0	38.0
14-15	36.56530430203441	38.0	37.0	38.0	34.0	38.0
16-17	36.520800868359686	38.0	37.0	38.0	34.0	38.0
18-19	36.52573375318381	38.0	37.0	38.0	34.0	38.0
20-21	36.55915700329933	38.0	37.0	38.0	34.0	38.0
22-23	36.43451837204054	38.0	37.0	38.0	34.0	38.0
24-25	36.40391084267429	38.0	37.0	38.0	34.0	38.0
26-27	36.30078775698826	38.0	37.0	38.0	34.0	38.0
28-29	36.16660039334114	38.0	37.0	38.0	34.0	38.0
30-31	36.078496276155576	38.0	37.0	38.0	33.5	38.0
32-33	36.10278455437458	38.0	37.0	38.0	34.0	38.0
34-35	36.04902793151995	38.0	37.0	38.0	33.0	38.0
36-37	36.12199486292168	38.0	37.0	38.0	34.0	38.0
38-39	35.86931616675085	38.0	37.0	38.0	32.0	38.0
40-41	36.08099495964492	38.0	37.0	38.0	34.0	38.0
42-43	36.19346258422981	38.0	37.0	38.0	34.0	38.0
44-45	36.16556867886813	38.0	37.0	38.0	34.0	38.0
46-47	36.21295768895958	38.0	37.0	38.0	34.0	38.0
48-49	36.207530440950464	38.0	37.0	38.0	34.0	38.0
50-51	36.15182323292029	38.0	37.0	38.0	34.0	38.0
52-53	36.16176423174886	38.0	37.0	38.0	34.0	38.0
54-55	36.150275661210756	38.0	37.0	38.0	34.0	38.0
56-57	36.14678825135144	38.0	37.0	38.0	34.0	38.0
58-59	36.117131833765	38.0	37.0	38.0	34.0	38.0
60-61	36.17908306376211	38.0	37.0	38.0	34.0	38.0
62-63	36.11449343894077	38.0	37.0	38.0	34.0	38.0
64-65	36.13989940783888	38.0	37.0	38.0	34.0	38.0
66-67	36.08773334479683	38.0	37.0	38.0	33.5	38.0
68-69	35.92678588700577	38.0	37.0	38.0	32.0	38.0
70-71	35.499715203817345	38.0	36.5	38.0	29.5	38.0
72-73	34.98294984363078	38.0	36.0	38.0	26.0	38.0
74-75	34.93181549506174	38.0	36.0	38.0	25.0	38.0
76	34.113864737933774	37.0	34.0	38.0	24.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	1.0
21	4.0
22	9.0
23	12.0
24	43.0
25	87.0
26	160.0
27	271.0
28	545.0
29	856.0
30	1382.0
31	2309.0
32	3390.0
33	5128.0
34	8041.0
35	12185.0
36	17393.0
37	41233.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.15841115971155	19.43814549323475	11.594966093133726	28.80847725391998
2	20.882545755462175	19.696074111489644	35.152446560414404	24.268933572633774
3	19.27479070167331	22.47525497318617	31.662887295940852	26.58706702919967
4	14.617029737020278	19.831486636073468	35.58125288826317	29.97023073864308
5	14.214016270997002	34.8719491880622	36.69894356736773	14.215090973573064
6	30.14755666369332	37.74892798418038	17.23715461745962	14.866360734666682
7	28.8417930337779	28.86651119302733	20.363464411224193	21.928231361970575
8	25.206074218959902	36.702167675095914	18.391385184150284	19.700372921793893
9	23.775645090221282	17.30056206944728	19.452116626723555	39.47167621360788
10-11	25.162011413341357	25.826714956635747	26.947092392180465	22.06418123784243
12-13	24.9723264086664	21.374759535298608	27.20072220013111	26.452191855903877
14-15	23.088372792829585	22.494599619555288	22.3199604509452	32.09706713666993
16-17	25.557501961332203	27.38019753033348	23.227546776429623	23.834753731904694
18-19	25.06851228922396	25.432836462509	24.4962331674709	25.00241808079614
20-21	22.40701135960623	25.17920665455835	24.04485808552483	28.368923900310588
22-23	23.427978806865198	27.136777396855422	24.258723898161183	25.176519898118194
24-25	25.440896731829465	27.505937731732743	24.00831819793872	23.044847338499068
26-27	24.591344345452395	28.322174338251887	24.975550516394588	22.110930799901126
28-29	22.291480832679554	27.991703296112803	25.625208223624114	24.091607647583533
30-31	21.78529591935432	25.315156530430205	27.89283065911509	25.00671689110039
32-33	21.985190598501863	27.504325677868653	25.18834162645488	25.322142097174606
34-35	22.571978205031755	24.910531010542833	27.853604015088823	24.663886769336585
36-37	25.19855130092747	26.410278455437457	25.1609367107653	23.23023353286978
38-39	22.344141258906596	25.46346548592677	27.86381368956141	24.328579565605217
40-41	22.187234682801535	23.80090060075874	28.83427011574547	25.17759460069426
42-43	24.688604928586013	23.9664047974723	28.472095347612548	22.87289492632914
44-45	21.973906221453213	26.37911208073166	28.61932960053305	23.02765209728208
46-47	24.398972584337283	23.466668099603435	27.01211189803222	25.12224741802706
48-49	23.810035572655266	23.187782781115327	26.86326559124762	26.13891605498178
50-51	21.665466582123397	23.23721909961418	29.329708003310085	25.767606314952335
52-53	20.688561940482973	23.01797977409752	32.511364979741856	23.782093305677655
54-55	20.476308181710714	23.439800535201883	30.293716214037765	25.790175069049642
56-57	22.71867510666423	23.475265720211933	27.659620200109618	26.14643897301422
58-59	20.396242839794088	24.036260464916335	31.392062246773207	24.175434448516373
60-61	22.349514771786907	26.85305591677503	27.16794377156122	23.62948553987684
62-63	19.591827961611624	30.91704370815377	25.432299111220967	24.05882921901364
64-65	19.583230341003127	31.344775333426472	24.860019989467915	24.211974336102486
66-67	19.59021590774753	30.93800040838698	24.46829090049329	25.0034927833722
68-69	19.268879837504972	31.42107921632688	24.388225558576664	24.921815387591483
70-71	19.864265064643362	30.45223484400692	24.170598286924093	25.512901804425624
72-73	20.142075680555408	28.761190340573243	24.200689959053832	26.896044019817516
74-75	19.867489172371545	28.576878848778602	24.545132134681726	27.01049984416813
76	20.01418607400402	28.642973057206415	24.27215768036196	27.0706831884276
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.5
8	1.0
9	0.5
10	0.0
11	0.0
12	0.0
13	0.0
14	0.5
15	1.0
16	4.0
17	7.0
18	11.0
19	17.0
20	29.5
21	40.0
22	44.0
23	69.0
24	140.5
25	191.0
26	240.5
27	339.5
28	492.5
29	596.0
30	682.5
31	895.5
32	1272.5
33	1523.0
34	1700.0
35	2056.5
36	2504.5
37	2773.0
38	3064.0
39	3924.0
40	5352.0
41	6605.0
42	6999.0
43	6884.0
44	6668.0
45	6141.0
46	5715.0
47	5582.5
48	5183.0
49	4580.5
50	4245.0
51	3952.5
52	3282.5
53	2616.5
54	2328.0
55	2170.5
56	1835.0
57	1479.5
58	1302.0
59	1155.5
60	951.0
61	880.5
62	868.0
63	822.0
64	799.0
65	834.5
66	870.0
67	893.0
68	838.5
69	792.0
70	798.5
71	797.0
72	776.5
73	713.0
74	658.0
75	646.0
76	601.5
77	506.5
78	400.0
79	344.0
80	298.0
81	200.0
82	132.5
83	117.0
84	91.5
85	45.5
86	23.5
87	22.0
88	19.0
89	11.5
90	5.0
91	2.0
92	1.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	93049.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.6894002084923
#Duplication Level	Percentage of deduplicated	Percentage of total
1	63.167420814479634	30.755838321744456
2	19.547511312217196	19.035132027211468
3	7.528970312327557	10.997431460843211
4	3.2888202185189273	6.405227353329966
5	1.7834676084317405	4.341798407290782
6	1.121289040944708	3.275693451837204
7	0.840966780708531	2.8662317703575533
8	0.6114115439796932	2.381540908553558
9	0.4348305926498179	1.9054476673580587
>10	1.64220284736784	11.666971165729883
>50	0.015450833241364089	0.6082816580511343
>100	0.013243571349740647	1.6356973207664778
>500	0.002207261891623441	0.6115057657793206
>1k	0.002207261891623441	3.513202721146923
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT	3269	3.513202721146923	No Hit
ATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTCTT	569	0.6115057657793206	RNA PCR Primer, Index 42 (95% over 22bp)
TCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG	481	0.516931939085858	No Hit
CTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTC	243	0.2611527259830842	RNA PCR Primer, Index 32 (95% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC	230	0.2471815924942772	No Hit
TCTTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC	225	0.24180807961396686	No Hit
CTTTACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTC	221	0.23750926930971852	No Hit
CTTATACACATCTCCGAGCCCACGAGACATCACTCGACCTCGTATGCCGT	122	0.13111371427957313	No Hit
CTTAATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG	97	0.10424614987802126	No Hit
TATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTCT	94	0.10102204214983504	RNA PCR Primer, Index 42 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0010747025760620749	0.0
5	0.0	0.0	0.0	0.004298810304248299	0.0
6	0.0	0.0	0.0	0.004298810304248299	0.0
7	0.0	0.0	0.0	0.004298810304248299	0.0
8	0.0	0.0	0.0	0.004298810304248299	0.0
9	0.0	0.0	0.0	0.004298810304248299	0.0
10	0.0	0.0	0.0	0.004298810304248299	0.0
11	0.0	0.0	0.0	0.004298810304248299	0.0
12	0.0	0.0	0.0	0.004298810304248299	0.0
13	0.0	0.0	0.0	0.004298810304248299	0.0
14	0.0	0.0	0.0	0.004298810304248299	0.0
15	0.0	0.0	0.0	0.004298810304248299	0.0
16	0.0	0.0	0.0	0.004298810304248299	0.0
17	0.0	0.0	0.0	0.0053735128803103745	0.0
18	0.0	0.0	0.0	0.006448215456372449	0.0
19	0.0	0.0	0.0	0.007522918032434524	0.0
20	0.0	0.0	0.0	0.008597620608496599	0.0
21	0.0	0.0	0.0	0.010747025760620749	0.0
22	0.0	0.0	0.0	0.012896430912744897	0.0
23	0.0	0.0	0.0	0.013971133488806972	0.0
24	0.0	0.0	0.0	0.015045836064869048	0.0
25	0.0	0.0	0.0	0.019344646369117348	0.0
26	0.0	0.0	0.0	0.021494051521241498	0.0
27	0.0	0.0	0.0	0.03116637470580017	0.0
28	0.0	0.0	0.0	0.04943631849885544	0.0
29	0.0	0.0	0.0	0.06878096486797279	0.0
30	0.0	0.0	0.0	0.09134971896527636	0.0
31	0.0	0.0	0.0	0.11929198594289031	0.0
32	0.0	0.0	0.0	0.14401014519231803	0.0
33	0.0	0.0	0.0	0.19452116626723553	0.0
34	0.0	0.0	0.0	0.24503218734215307	0.0
35	0.0	0.0	0.0	0.3191866650904362	0.0
36	0.0	0.0	0.0	0.47501853861943705	0.0
37	0.0	0.0	0.0	0.7082289976249073	0.0
38	0.0	0.0	0.0	1.0521338219647713	0.0
39	0.0	0.0	0.0	1.3584240561424625	0.0
40	0.0	0.0	0.0	1.7743339530784856	0.0
41	0.0	0.0	0.0	2.3578974518801923	0.0
42	0.0	0.0	0.0	3.005943105245623	0.0
43	0.0	0.0	0.0	3.704499779685972	0.0
44	0.0	0.0	0.0	4.333200786682285	0.0
45	0.0	0.0	0.0	5.0543262152199375	0.0
46	0.0	0.0	0.0	5.794796290126707	0.0
47	0.0	0.0	0.0	6.590076196412642	0.0
48	0.0	0.0	0.0	7.306902814646047	0.0
49	0.0	0.0	0.0	8.030177648335822	0.0
50	0.0	0.0	0.0	8.730883727928296	0.0
51	0.0	0.0	0.0	9.52079012133392	0.0
52	0.0	0.0	0.0	10.293501273522553	0.0
53	0.0	0.0	0.0	11.097378800416985	0.0
54	0.0	0.0	0.0	11.904480435039602	0.0
55	0.0	0.0	0.0	12.62238175584907	0.0
56	0.0	0.0	0.0	13.304817891648486	0.0
57	0.0	0.0	0.0	13.93889241152511	0.0
58	0.0	0.0	0.0	14.613805629292093	0.0
59	0.0	0.0	0.0	15.268299498113898	0.0
60	0.0	0.0	0.0	15.952885039065439	0.0
61	0.0	0.0	0.0	16.614901825919677	0.0
62	0.0	0.0	0.0	17.286590935958472	0.0
63	0.0	0.0	0.0	17.98407290782276	0.0
64	0.0	0.0	0.0	18.67940547453492	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTCCCAT	15	0.002218463	70.0	50
AGTTCAT	15	0.002218463	70.0	12
TTCGACG	15	0.002218463	70.0	10
CCTCGTA	20	0.0069320644	52.5	38
TATAGGG	20	0.0069320644	52.5	2
TAGCTCA	20	0.0069320644	52.5	31
AATGCCG	35	0.0012498279	40.0	40
CTTAATA	45	9.136822E-5	38.88889	2
GCCTATG	45	9.136822E-5	38.88889	1
CTTTATA	45	9.136822E-5	38.88889	2
GGCTATG	50	1.6984473E-4	35.000004	1
CTATACA	60	1.204589E-5	35.0	1
TCTAGCA	40	0.0024080568	35.0	3
CATACCT	40	0.0024080568	35.0	41
TATGCCG	745	0.0	34.2953	43
GTATGCC	745	0.0	34.2953	42
TCGTATG	745	0.0	34.2953	40
CTCGTAT	735	0.0	34.285717	39
CGTCTTC	735	0.0	34.285717	48
CCGTCTT	735	0.0	34.285717	47
>>END_MODULE