##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779737_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	62692
Sequences flagged as poor quality	0
Sequence length	76
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.138550373253366	33.0	23.0	34.0	12.0	34.0
2	27.025792764627067	32.0	23.0	34.0	12.0	34.0
3	26.116617750271168	32.0	21.0	34.0	11.0	34.0
4	28.020879857079052	33.0	23.0	34.0	12.0	34.0
5	27.731895616665604	33.0	23.0	34.0	11.0	34.0
6	29.797916799591654	35.0	24.0	38.0	11.0	38.0
7	30.857079053148727	36.0	26.0	38.0	12.0	38.0
8	30.056945064761052	36.0	24.0	38.0	11.0	38.0
9	31.54008485931219	37.0	27.0	38.0	12.0	38.0
10-11	31.12373987111593	37.0	26.0	38.0	11.0	38.0
12-13	30.42369042302048	35.5	25.0	38.0	11.0	38.0
14-15	30.164558476360618	35.0	24.5	38.0	11.0	38.0
16-17	29.92282109360046	35.5	24.0	38.0	11.0	38.0
18-19	30.476121355196835	36.0	25.5	38.0	11.0	38.0
20-21	29.43946596056913	35.0	23.5	38.0	11.0	38.0
22-23	29.45582370956422	35.0	23.5	38.0	11.0	38.0
24-25	30.440311363491354	36.0	24.5	38.0	11.0	38.0
26-27	29.10788457857462	34.5	22.0	38.0	11.0	38.0
28-29	30.920891022778026	37.0	26.0	38.0	11.0	38.0
30-31	31.38311108275378	37.0	26.5	38.0	11.0	38.0
32-33	31.510822752504307	37.0	27.0	38.0	11.0	38.0
34-35	31.570120589548907	37.0	27.0	38.0	11.0	38.0
36-37	29.715155043705735	35.0	23.5	38.0	11.0	38.0
38-39	28.316435908887897	34.0	21.0	37.5	11.0	38.0
40-41	29.151646143048552	34.0	22.0	37.5	11.0	38.0
42-43	30.07332674025394	35.5	24.0	38.0	11.0	38.0
44-45	31.574531040643144	37.0	27.0	38.0	11.0	38.0
46-47	30.38711478338544	35.5	24.5	38.0	11.0	38.0
48-49	29.686092324379505	35.0	23.5	38.0	11.0	38.0
50-51	27.794718624385887	33.0	21.0	37.5	11.0	38.0
52-53	30.65400689083137	36.5	25.0	38.0	11.0	38.0
54-55	29.85549192879474	35.5	23.5	38.0	11.0	38.0
56-57	30.301960377719645	36.0	24.0	38.0	11.0	38.0
58-59	31.203494863778474	37.0	26.0	38.0	11.0	38.0
60-61	31.728426274484782	37.5	27.0	38.0	11.0	38.0
62-63	31.65731672302686	37.5	27.0	38.0	11.0	38.0
64-65	31.384243603649587	37.5	26.0	38.0	11.0	38.0
66-67	29.566738977860012	35.5	23.0	38.0	11.0	38.0
68-69	29.582020034454157	35.0	23.0	38.0	11.0	38.0
70-71	29.15702162955401	34.5	22.0	38.0	11.0	38.0
72-73	28.184800293498373	33.5	21.0	37.5	11.0	38.0
74-75	29.899580488738593	35.5	24.0	37.5	11.0	38.0
76	30.11942512601289	36.0	24.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	28.0
13	177.0
14	490.0
15	829.0
16	1414.0
17	2008.0
18	2517.0
19	2566.0
20	2306.0
21	1750.0
22	1268.0
23	1022.0
24	927.0
25	826.0
26	901.0
27	1040.0
28	1212.0
29	1453.0
30	1812.0
31	2384.0
32	3296.0
33	4499.0
34	6213.0
35	8028.0
36	9020.0
37	4705.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.66949531040643	19.048682447521216	14.159701397307472	32.122120844764886
2	18.381930708862374	16.95591144005615	42.58119058253047	22.08096726855101
3	18.50634849741594	18.008677343201683	39.228290690997255	24.25668346838512
4	14.13736999936196	20.557646908696483	31.713775282332673	33.59120780960888
5	14.269763287181778	28.02430932176354	39.92056402730811	17.78536336374657
6	25.921967715178972	33.66617750271167	17.482294391628916	22.929560390480443
7	25.089325591782046	24.888343010272443	20.48746251515345	29.534868882792065
8	21.292987941045112	32.715497990174185	18.814202769093345	27.17731129968736
9	21.635934409494034	14.98117782173164	20.23384163848657	43.14904613028776
10-11	23.999074842085115	23.488642889044854	27.077617558859185	25.434664710010846
12-13	22.939928539526573	18.602054488610985	30.17450392394564	28.2835130479168
14-15	24.42177630319658	22.236489504242964	23.074714477126268	30.267019715434184
16-17	26.368595674089196	21.103171058508263	24.86999936196006	27.65823390544248
18-19	21.727652651055955	20.94685127288968	28.477317680086774	28.84817839596759
20-21	25.87331716965482	20.76660498947234	25.950679512537484	27.409398328335357
22-23	26.98111401773751	19.824698526127733	25.6436227907867	27.55056466534805
24-25	22.69109296241945	23.863491354558793	22.77882345434824	30.666592228673515
26-27	25.11085943980093	22.90244369297518	25.639635041153575	26.347061826070313
28-29	23.212690614432464	23.833982007273654	25.139571237159448	27.813756141134434
30-31	22.84502009825815	23.172813118101192	25.575831047023545	28.406335736617113
32-33	23.349869201812034	22.95348688827921	25.259203726153256	28.437440183755502
34-35	23.44078989344733	22.747719007209852	25.49687360428763	28.314617495055188
36-37	25.74331653161488	19.71623173610668	29.79806035857845	24.74239137369999
38-39	25.288713073438394	18.458495501818415	33.549735213424356	22.703056211318827
40-41	23.866074348197124	23.346067648724308	24.369332365631706	28.418525637446862
42-43	20.620653352899893	23.66968672238882	26.77773878644803	28.931921138263256
44-45	20.833599183308877	21.316116888917247	28.992534932686787	28.85774899508709
46-47	24.993619600586996	20.2976456326166	27.697313851847127	27.011420914949273
48-49	24.964110253301858	20.76102213998596	29.14087921903911	25.133988387673067
50-51	29.33627895106234	18.627576086263	28.923945638997	23.11219932367766
52-53	20.05439290499585	22.360109742869906	28.650386014164486	28.935111337969754
54-55	24.11073183181267	20.979550819881325	27.22037899572513	27.689338352580872
56-57	24.357174759139923	21.363172334588146	26.05914630255854	28.220506603713392
58-59	20.674089197983793	23.03962228035475	27.812161041281186	28.474127480380275
60-61	20.681267147323425	21.43335672813118	27.832897339373446	30.052478785171953
62-63	19.877336821285013	23.014100682702736	27.54338671600842	29.565175780003827
64-65	20.51218656287884	24.368340458112677	26.3717858737957	28.747687105212787
66-67	19.45782555988005	26.690008294519235	22.132010463855035	31.720155681745677
68-69	19.40033022517528	26.93967408210961	22.399476744649792	31.260518948065315
70-71	19.52880750334971	27.29215848912142	21.495565622407963	31.68346838512091
72-73	18.76475467364257	27.704491801186755	20.442799719262425	33.08795380590825
74-75	20.676481847763668	24.755949722452623	23.321954954380146	31.245613475403562
76	20.736298092260576	23.286862757608624	24.68895552861609	31.287883621514705
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.5
14	1.0
15	0.5
16	1.0
17	2.0
18	3.5
19	6.5
20	16.0
21	24.0
22	24.0
23	29.0
24	49.5
25	65.0
26	87.0
27	130.5
28	202.0
29	252.0
30	306.5
31	431.0
32	575.0
33	649.0
34	778.0
35	1054.0
36	1356.0
37	1511.0
38	1697.0
39	2046.5
40	2465.5
41	2866.0
42	3011.0
43	3087.0
44	3225.5
45	3390.5
46	3493.0
47	3465.5
48	3362.0
49	3102.5
50	2919.0
51	2812.5
52	2508.5
53	2178.0
54	2045.0
55	1944.5
56	1700.5
57	1480.0
58	1403.0
59	1374.0
60	1301.5
61	1247.5
62	1237.0
63	1234.5
64	1227.5
65	1218.0
66	1190.0
67	1167.0
68	1124.5
69	1044.0
70	968.0
71	930.0
72	890.5
73	810.5
74	694.5
75	619.0
76	582.0
77	473.0
78	336.5
79	272.0
80	227.0
81	146.0
82	95.5
83	81.0
84	60.0
85	28.0
86	15.0
87	13.0
88	10.5
89	6.0
90	2.5
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	7.975499266254068E-4
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.011963248899381102
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	62692.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	96.62476871052128
#Duplication Level	Percentage of deduplicated	Percentage of total
1	97.38840464870576	94.1013207426785
2	2.047015319598521	3.9558476360620176
3	0.37143423137876386	1.076692400944299
4	0.11885895404120445	0.4593887577362342
5	0.0462229265715795	0.2233139794551139
6	0.016508188061278396	0.09570599119504881
7	0.003301637612255679	0.022331397945511387
8	0.006603275224511358	0.05104319530402603
9	0.0016508188061278395	0.01435589867925732
>10	0.0	0.0
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.003190199706501627	0.0
2	0.0	0.0	0.0	0.003190199706501627	0.0
3	0.0	0.0	0.0	0.006380399413003254	0.0
4	0.0	0.0	0.0	0.00957059911950488	0.0
5	0.0	0.0	0.0	0.011165698972755694	0.0
6	0.0	0.0	0.0	0.012760798826006508	0.0
7	0.0	0.0	0.0	0.012760798826006508	0.0
8	0.0	0.0	0.0	0.012760798826006508	0.0
9	0.0	0.0	0.0	0.015950998532508136	0.0
10	0.0	0.0	0.0	0.015950998532508136	0.0
11	0.0	0.0	0.0	0.01754609838575895	0.0
12	0.0	0.0	0.0	0.01754609838575895	0.0
13	0.0	0.0	0.0	0.01914119823900976	0.0
14	0.0	0.0	0.0	0.028711797358514642	0.0
15	0.0	0.0	0.0	0.028711797358514642	0.0
16	0.0	0.0	0.0	0.028711797358514642	0.0
17	0.0	0.0	0.0	0.028711797358514642	0.0
18	0.0	0.0	0.0	0.030306897211765455	0.0
19	0.0	0.0	0.0	0.03190199706501627	0.0
20	0.0	0.0	0.0	0.033497096918267084	0.0
21	0.0	0.0	0.0	0.0350921967715179	0.0
22	0.0	0.0	0.0	0.0590186945702801	0.0
23	0.0	0.0	0.0	0.06220889427678172	0.0
24	0.0	0.0	0.0	0.07975499266254067	0.0
25	0.0	0.0	0.0	0.08454029222229312	0.0
26	0.0	0.0	0.0	0.08613539207554392	0.0
27	0.0	0.0	0.0	0.11165698972755694	0.0
28	0.0	0.0	0.0	0.13558348752631916	0.0
29	0.0	0.0	0.0	0.15791488547183052	0.0
30	0.0	0.0	0.0	0.18662668283034517	0.0
31	0.0	0.0	0.0	0.1993874816563517	0.0
32	0.0	0.0	0.0	0.23926497798762203	0.0
33	0.0	0.0	0.0	0.2919032731448989	0.0
34	0.0	0.0	0.0	0.3429464684489249	0.0
35	0.0	0.0	0.0	0.4514132584699802	0.0
36	0.0	0.0	0.0	0.547119249665029	0.0
37	0.0	0.0	0.0	0.8342372232501755	0.0
38	0.0	0.0	0.0	1.0591463025585401	0.0
39	0.0	0.0	0.0	1.3303132776111786	0.0
40	0.0	0.0	0.0	1.633382249728833	0.0
41	0.0	0.0	0.0	2.009825815096025	0.0
42	0.0	0.0	0.0	2.4070056785554774	0.0
43	0.0	0.0	0.0	2.7276207490588913	0.0
44	0.0	0.0	0.0	3.1184202131053405	0.0
45	0.0	0.0	0.0	3.429464684489249	0.0
46	0.0	0.0	0.0	3.7676258533784215	0.0
47	0.0	0.0	0.0	4.198302813756142	0.0
48	0.0	0.0	0.0	4.443948191156767	0.0
49	0.0	0.0	0.0	4.731066164741913	0.0
50	0.0	0.0	0.0	5.147387226440375	0.0
51	0.0	0.0	0.0	5.54935238945958	0.0
52	0.0	0.0	0.0	5.927391054680023	0.0
53	0.0	0.0	0.0	6.3819945128565045	0.0
54	0.0	0.0	0.0	6.820646972500478	0.0
55	0.0	0.0	0.0	7.169973840362407	0.0
56	0.0	0.0	0.0	7.535251706756843	0.0
57	0.0	0.0	0.0	7.78408728386397	0.0
58	0.0	0.0	0.0	8.013781662732088	0.0
59	0.0	0.0	0.0	8.420532125311045	0.0
60	0.0	0.0	0.0	8.659797103298667	0.0
61	0.0	0.0	0.0	8.876730683340778	0.0
62	0.0	0.0	0.0	9.036240668665858	0.0
63	0.0	0.0	0.0	9.227652651055957	0.0
64	0.0	0.0	0.0	9.465322529190328	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCATGTG	15	0.0022157726	69.994415	32
CTTGGTT	15	0.0022157726	69.994415	28
GTAGATT	20	7.8734556E-5	69.994415	38
ACGTGTA	15	0.0022157726	69.994415	34
CGTACCA	15	0.0022157726	69.994415	56
CGTGTAT	20	7.8734556E-5	69.994415	35
GCGTGTA	15	0.0022157726	69.994415	34
TTTGAGC	15	0.0022157726	69.994415	26
CGACTTG	15	0.0022157726	69.994415	25
TCGCGTA	20	7.8734556E-5	69.994415	34
ACGACTT	25	2.8110753E-6	69.994415	24
TCATTAA	45	3.765308E-10	62.21726	60
ACGCTTC	45	3.765308E-10	62.21726	15
CTCTCGG	40	1.0461008E-8	61.245113	42
GACGACT	35	2.9320654E-7	59.995213	23
TCGCTGG	30	8.292283E-6	58.328682	45
CACGTGT	30	8.292283E-6	58.328682	33
TGTGTAG	30	8.292283E-6	58.328682	35
AAGTGTA	30	8.292283E-6	58.328682	34
GCCGTAT	60	1.8189894E-12	58.328682	54
>>END_MODULE