FastQCFastQC Report
Wed 25 May 2016
SRR1779737_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779737_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62692
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT910714.52657436355516No Hit
TCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG12331.9667581190582528No Hit
ATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTCTT7821.2473680852421363RNA PCR Primer, Index 15 (95% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC6130.9777962100427486No Hit
CTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTC4370.6970586358706056No Hit
TCTTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC4030.6428252408600779No Hit
CTTTACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTC3260.5200025521597652No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAACCTCGTATGCCGT1890.30147387226440375No Hit
CTTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG1690.26957187519938747No Hit
TTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTC1610.25681107637338096No Hit
TCTTAATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC1530.24405027754737443No Hit
CTTAATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG1480.23607477828112042No Hit
TATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTCT1480.23607477828112042No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAAACTCGTATGCCGT860.13717858737956995No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAATATCGTATGCCGT830.13239328781981752No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTGTTA150.002215071870.047
GTAGGCA150.002215071870.033
TATGCAA150.002215071870.02
TAGGGAT150.002215071870.067
GCAATGA150.002215071870.05
TGTCGTA150.002215071870.029
TGCAATG150.002215071870.04
TCTATCA150.002215071870.062
TCTTTAT401.0453732E-861.2500041
TTAATAC252.3735693E-455.9999963
CTATACA700.055.01
TTTACAC900.054.4444432
CTTTATA452.6488124E-854.4444432
GTTTGTC200.00692154952.50000426
GAGCTTG200.00692154952.50000444
ATAGGGA200.00692154952.50000466
TTGTCGT200.00692154952.50000428
GTATGCA200.00692154952.5000041
CTCATAG200.00692154952.50000463
AGCGATT200.00692154952.50000411
Produced by FastQC (version 0.11.5)