FastQCFastQC Report
Wed 25 May 2016
SRR1779736_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779736_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences163024
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTTT308.348006E-658.33333244
TCCGAAC200.006941392652.53
TGTGGAC200.006941392652.537
ACTTCGT200.006941392652.533
AGCGCAT200.006941392652.510
CAGTGTA200.006941392652.534
AGATCTC750.051.33333240
AACGCTG352.0795253E-550.014
CGTATCA751.4551915E-1146.66666856
CTGTGTA451.6768372E-646.66666834
TTCCGCT551.3033423E-744.54545213
GTCGCCG901.8189894E-1242.7777851
TCGCCGT953.6379788E-1240.52631852
ACCTGAT350.001252601740.025
TGCCTAC701.9108484E-840.019
GAACCGA350.001252601740.033
GATCTCG1150.039.56521641
GTATAAA459.1671885E-538.888891
TTACGCT556.663915E-638.18181613
CTTCCGC654.8359834E-737.69230712
Produced by FastQC (version 0.11.5)