##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779736_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	163024
Sequences flagged as poor quality	0
Sequence length	76
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.400198743743253	34.0	32.0	34.0	21.0	34.0
2	28.591557071351456	34.0	23.0	34.0	12.0	34.0
3	27.42823756011385	33.0	23.0	34.0	11.0	34.0
4	30.09596746491314	34.0	31.0	34.0	21.0	34.0
5	29.621712140543725	34.0	31.0	34.0	11.0	34.0
6	32.20091520266955	37.0	31.0	38.0	12.0	38.0
7	33.23596525664933	37.0	34.0	38.0	21.0	38.0
8	32.459938413975856	37.0	31.0	38.0	12.0	38.0
9	33.85921704779664	37.0	34.0	38.0	21.0	38.0
10-11	33.67977414368437	38.0	34.0	38.0	21.0	38.0
12-13	32.50551145843557	37.5	30.0	38.0	17.0	38.0
14-15	32.68899671214054	37.0	32.5	38.0	16.0	38.0
16-17	32.317048410050056	37.0	30.0	38.0	16.5	38.0
18-19	32.93810727254883	37.0	32.5	38.0	16.0	38.0
20-21	31.74298569535774	36.5	29.0	38.0	16.0	38.0
22-23	31.715796447148886	36.5	28.5	38.0	16.0	38.0
24-25	33.08853911080577	37.0	32.5	38.0	21.0	38.0
26-27	31.557022278928255	37.0	28.5	38.0	11.0	38.0
28-29	33.84025971636078	37.5	34.0	38.0	22.0	38.0
30-31	34.28964446952595	38.0	35.5	38.0	23.0	38.0
32-33	34.37049759544607	38.0	36.0	38.0	23.0	38.0
34-35	34.426602831484935	38.0	36.0	38.0	23.5	38.0
36-37	32.141865614878796	37.0	30.0	38.0	16.0	38.0
38-39	30.20771174796349	35.0	24.5	38.0	11.0	38.0
40-41	31.648959662381	36.5	29.0	38.0	11.0	38.0
42-43	32.753757115516734	37.0	30.5	38.0	17.0	38.0
44-45	34.47788055746393	38.0	35.5	38.0	24.5	38.0
46-47	32.91999644224163	37.5	31.0	38.0	18.0	38.0
48-49	32.36637243596035	37.0	30.0	38.0	17.0	38.0
50-51	29.987066321523212	35.0	23.5	38.0	11.0	38.0
52-53	33.68384102954166	37.0	34.0	38.0	22.0	38.0
54-55	32.64854561291589	37.0	30.5	38.0	17.0	38.0
56-57	33.09683236824026	37.5	31.5	38.0	17.5	38.0
58-59	34.283430660516245	38.0	35.0	38.0	23.5	38.0
60-61	34.721372313279026	38.0	36.0	38.0	25.0	38.0
62-63	34.61521003042496	38.0	36.0	38.0	24.5	38.0
64-65	34.30659289429777	38.0	35.0	38.0	23.0	38.0
66-67	32.03097396702326	37.0	29.0	38.0	11.0	38.0
68-69	32.18240259102954	37.0	29.5	38.0	11.0	38.0
70-71	31.68653388458141	37.0	28.0	38.0	11.0	38.0
72-73	30.380572799097067	35.5	24.5	38.0	11.0	38.0
74-75	32.813229953871826	37.0	31.5	38.0	17.0	38.0
76	33.27844366473648	37.0	34.0	38.0	21.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	2.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	4.0
12	25.0
13	149.0
14	411.0
15	732.0
16	1245.0
17	1874.0
18	2506.0
19	2595.0
20	2411.0
21	2026.0
22	1708.0
23	1584.0
24	1743.0
25	1844.0
26	2272.0
27	2782.0
28	3491.0
29	4261.0
30	5604.0
31	7435.0
32	10156.0
33	13869.0
34	19356.0
35	25863.0
36	30121.0
37	16955.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	40.95593286878006	19.26648836981058	11.434512709785063	28.343066051624298
2	21.96425066247914	17.33732456570812	37.12091471194425	23.577510059868487
3	20.74418490529002	18.876975169300223	34.56055550103052	25.818284424379236
4	13.950706644420455	18.688045931887327	35.23836981058004	32.12287761311218
5	14.240234566689566	31.14940131514378	38.71025125134949	15.900112866817157
6	27.77443811954068	33.83857591520267	17.466139954853276	20.920846010403377
7	28.11119835116302	27.437064481303363	21.42629306114437	23.02544410638924
8	24.420630344370696	31.993841322030153	18.250910919998528	25.33461741360062
9	23.810283274650047	16.66523536700568	20.75425402706383	38.77022733128044
10-11	24.992485630685618	24.49899092754921	27.179933873550034	23.32858956821514
12-13	25.2505796763627	21.169228693059832	28.25845591392573	25.321735716651737
14-15	23.244715437180258	23.141355154519022	24.333218829360455	29.280710578940266
16-17	23.772543246227457	24.457428536375904	27.49723960250276	24.272788614893877
18-19	22.430614554611232	24.49017148150079	27.96151404271242	25.117699921175557
20-21	23.195335598876227	24.238139637594927	28.411196034890995	24.155328728637855
22-23	23.842867351852362	23.564989893971042	28.965197842002432	23.62694491217416
24-25	22.500506063561584	25.618164308103765	25.45929102028548	26.42203860804917
26-27	22.093337095606728	24.867195838598473	28.41579664094417	24.623670424850634
28-29	22.936168124547606	25.59317147378881	27.472672400044168	23.997988001619415
30-31	22.650149367259434	25.6289067052711	27.033020285730057	24.687923641739406
32-33	22.278963204239933	25.84137675091936	26.988771419720713	24.89088862512
34-35	23.126326508078666	25.274809534909398	27.37789991534885	24.220964041663088
36-37	23.347298814263194	23.72945816796609	30.096122585433775	22.82712043233694
38-39	23.040353820531774	22.785784609911023	32.774098963013856	21.399762606543348
40-41	23.041273950208716	23.55286605058873	26.657690290484936	26.74816970871762
42-43	21.485136975377557	24.01792396118315	27.332813976027776	27.164125087411517
44-45	21.315900049993406	23.98640670095662	29.21424474685854	25.48344850219143
46-47	22.49389652930279	22.732207922857036	30.358785930733273	24.415109617106896
48-49	22.383857343969968	23.511622700073303	30.348450971190914	23.756068984765815
50-51	22.654987225611343	22.23909116282208	32.7171569394221	22.38876467214447
52-53	20.45233574918492	24.242963308295582	29.711293978364683	25.593406964154813
54-55	21.075812318658333	24.15394335699082	29.522576845927823	25.247667478423025
56-57	21.02691661248175	24.370330384856032	29.126436922623938	25.476316080038274
58-59	19.964544553939202	24.915731974003428	29.062730989470715	26.056992482586654
60-61	19.804871166509734	24.35184532006711	29.241107713447086	26.602175799976074
62-63	19.77720661755234	24.811527349237213	28.437747284092236	26.973518749118213
64-65	20.56838954251573	24.861981818404878	27.490154703046215	27.07947393603317
66-67	19.62955351967096	23.60392468385684	25.00222366035965	31.76429813611255
68-69	20.041352737624855	24.297801065109088	24.92177338208948	30.739072815176577
70-71	20.483557333104326	23.573036602646287	24.637316664724178	31.306089399525217
72-73	20.06698502958199	23.233131826170165	23.154614575378094	33.54526856886975
74-75	21.358839171520323	24.881380676781898	24.430826608760196	29.328953542937587
76	22.029063740254323	23.888333404898756	24.964881825040948	29.117721029805978
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.5
3	1.0
4	1.0
5	0.5
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.5
16	3.0
17	5.0
18	7.0
19	12.5
20	26.0
21	36.0
22	53.5
23	82.0
24	132.0
25	171.0
26	221.5
27	341.0
28	512.0
29	614.0
30	753.5
31	1082.5
32	1536.0
33	1800.0
34	2221.5
35	3089.0
36	4040.5
37	4546.0
38	5217.5
39	6592.0
40	7951.5
41	9098.5
42	9589.0
43	9886.0
44	10469.0
45	10980.5
46	11206.0
47	11009.5
48	10452.5
49	9609.5
50	9127.0
51	8551.5
52	7353.5
53	6120.5
54	5510.0
55	5058.0
56	4062.5
57	3249.5
58	2980.0
59	2679.0
60	2210.0
61	1940.5
62	1839.0
63	1758.5
64	1640.5
65	1553.5
66	1510.5
67	1517.0
68	1459.5
69	1384.0
70	1285.5
71	1205.0
72	1168.5
73	1071.0
74	920.5
75	831.0
76	744.0
77	573.5
78	433.5
79	377.0
80	326.5
81	221.5
82	144.5
83	122.0
84	97.5
85	53.5
86	30.0
87	26.0
88	18.5
89	7.0
90	2.0
91	1.5
92	2.0
93	1.5
94	0.5
95	0.0
96	0.0
97	1.5
98	3.5
99	3.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.001226813229953872
9	0.001226813229953872
10-11	0.0018402198449308078
12-13	0.001226813229953872
14-15	0.001226813229953872
16-17	0.002453626459907744
18-19	0.0021469231524192754
20-21	0.001226813229953872
22-23	0.00153351653744234
24-25	6.13406614976936E-4
26-27	0.001226813229953872
28-29	0.001226813229953872
30-31	0.0018402198449308078
32-33	0.00153351653744234
34-35	0.001226813229953872
36-37	0.0018402198449308078
38-39	0.0021469231524192754
40-41	0.0021469231524192754
42-43	0.001226813229953872
44-45	0.00153351653744234
46-47	0.001226813229953872
48-49	0.00153351653744234
50-51	0.00153351653744234
52-53	0.0021469231524192754
54-55	0.0018402198449308078
56-57	0.001226813229953872
58-59	0.00153351653744234
60-61	0.0021469231524192754
62-63	0.0018402198449308078
64-65	0.001226813229953872
66-67	0.0027603297673962114
68-69	0.02208263813916969
70-71	0.0018402198449308078
72-73	0.00153351653744234
74-75	0.00153351653744234
76	0.0018402198449308078
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	163024.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.5617640838312
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.29839264079479	80.81810081730096
2	4.951641070149851	8.671488544245415
3	1.3887877362551515	3.648141123734747
4	0.5815419235987427	2.03683346876042
5	0.295512653832521	1.293780463933504
6	0.15722576395170865	0.8260179150623788
7	0.08945803121500163	0.5483172117256386
8	0.08048081927860569	0.5637634008757381
9	0.04967280883996264	0.3914494892123448
>10	0.10728655208364982	1.20210756514884
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0018402198449308078	0.0
5	0.0	0.0	0.0	0.0018402198449308078	0.0
6	0.0	0.0	0.0	0.002453626459907744	0.0
7	0.0	0.0	0.0	0.0030670330748846794	0.0
8	0.0	0.0	0.0	0.0030670330748846794	0.0
9	0.0	0.0	0.0	0.0036804396898616155	0.0
10	0.0	0.0	0.0	0.004293846304838552	0.0
11	0.0	0.0	0.0	0.004293846304838552	0.0
12	0.0	0.0	0.0	0.004293846304838552	0.0
13	0.0	0.0	0.0	0.004293846304838552	0.0
14	0.0	0.0	0.0	0.006134066149769359	0.0
15	0.0	0.0	0.0	0.006747472764746295	0.0
16	0.0	0.0	0.0	0.006747472764746295	0.0
17	0.0	0.0	0.0	0.006747472764746295	0.0
18	0.0	0.0	0.0	0.007360879379723231	0.0
19	0.0	0.0	0.0	0.007974285994700167	0.0
20	0.0	0.0	0.0	0.00920109922465404	0.0
21	0.0	0.0	0.0	0.010427912454607911	0.0
22	0.0	0.0	0.0	0.019015605064285013	0.0
23	0.0	0.0	0.0	0.020242418294238885	0.0
24	0.0	0.0	0.0	0.02269604475414663	0.0
25	0.0	0.0	0.0	0.0239228579841005	0.0
26	0.0	0.0	0.0	0.026376484444008245	0.0
27	0.0	0.0	0.0	0.051526155658062615	0.0
28	0.0	0.0	0.0	0.07299538718225537	0.0
29	0.0	0.0	0.0	0.09017077240160958	0.0
30	0.0	0.0	0.0	0.10857297085091766	0.0
31	0.0	0.0	0.0	0.12268132299538718	0.0
32	0.0	0.0	0.0	0.14415055451957995	0.0
33	0.0	0.0	0.0	0.16807341250368044	0.0
34	0.0	0.0	0.0	0.20242418294238884	0.0
35	0.0	0.0	0.0	0.2631514378251055	0.0
36	0.0	0.0	0.0	0.3300127588575915	0.0
37	0.0	0.0	0.0	0.5766022180783198	0.0
38	0.0	0.0	0.0	0.842207282363333	0.0
39	0.0	0.0	0.0	1.1924624595151634	0.0
40	0.0	0.0	0.0	1.6482235744430267	0.0
41	0.0	0.0	0.0	2.250588870350378	0.0
42	0.0	0.0	0.0	2.9326970262047305	0.0
43	0.0	0.0	0.0	3.6405682598881146	0.0
44	0.0	0.0	0.0	4.30611443713809	0.0
45	0.0	0.0	0.0	4.7845715968201	0.0
46	0.0	0.0	0.0	5.38325645303759	0.0
47	0.0	0.0	0.0	6.03592109137305	0.0
48	0.0	0.0	0.0	6.537687702424183	0.0
49	0.0	0.0	0.0	6.982407498282462	0.0
50	0.0	0.0	0.0	7.7154284031799	0.0
51	0.0	0.0	0.0	8.52819216802434	0.0
52	0.0	0.0	0.0	9.272254391991364	0.0
53	0.0	0.0	0.0	10.246957503189714	0.0
54	0.0	0.0	0.0	11.108180390617333	0.0
55	0.0	0.0	0.0	11.663926783786437	0.0
56	0.0	0.0	0.0	12.234394935714986	0.0
57	0.0	0.0	0.0	12.671753852193541	0.0
58	0.0	0.0	0.0	13.119540681126706	0.0
59	0.0	0.0	0.0	13.656884875846501	0.0
60	0.0	0.0	0.0	14.007753459613308	0.0
61	0.0	0.0	0.0	14.369663362449701	0.0
62	0.0	0.0	0.0	14.645696339189321	0.0
63	0.0	0.0	0.0	15.029688880164883	0.0
64	0.0	0.0	0.0	15.436377465894592	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCGTTT	30	8.348006E-6	58.333332	44
TCCGAAC	20	0.0069413926	52.5	3
TGTGGAC	20	0.0069413926	52.5	37
ACTTCGT	20	0.0069413926	52.5	33
AGCGCAT	20	0.0069413926	52.5	10
CAGTGTA	20	0.0069413926	52.5	34
AGATCTC	75	0.0	51.333332	40
AACGCTG	35	2.0795253E-5	50.0	14
CGTATCA	75	1.4551915E-11	46.666668	56
CTGTGTA	45	1.6768372E-6	46.666668	34
TTCCGCT	55	1.3033423E-7	44.545452	13
GTCGCCG	90	1.8189894E-12	42.77778	51
TCGCCGT	95	3.6379788E-12	40.526318	52
ACCTGAT	35	0.0012526017	40.0	25
TGCCTAC	70	1.9108484E-8	40.0	19
GAACCGA	35	0.0012526017	40.0	33
GATCTCG	115	0.0	39.565216	41
GTATAAA	45	9.1671885E-5	38.88889	1
TTACGCT	55	6.663915E-6	38.181816	13
CTTCCGC	65	4.8359834E-7	37.692307	12
>>END_MODULE