FastQCFastQC Report
Wed 25 May 2016
SRR1779736_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779736_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences163024
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT84905.207822161154186No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG11160.6845617823142605No Hit
ATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTCTT7010.4299980370988321Illumina PCR Primer Index 9 (95% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC6560.40239473942487No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC3930.2410687996859358No Hit
CTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTC3920.24045539307095887No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGACCTCGTATGCCGT2590.1588723132790264No Hit
CTTTACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTC2510.1539650603592109No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTAGAG150.002221471470.018
TTAATAC452.6844646E-854.4444473
ATGGACA200.006941392652.58
GAACGTA200.006941392652.51
GCGATAA200.006941392652.511
GGTGCAA200.006941392652.523
TAGGACG200.006941392652.54
CAATACG200.006941392652.569
ACCCAGA200.006941392652.512
GAGGCAC200.006941392652.522
CTATAAC552.2664608E-950.909094
ATCTCGT13300.047.89473737
TCTCGTA13400.047.53731538
TCTTCTG13950.047.41935350
CTCGTAT13850.047.25631339
CGTATGC14000.047.2541
CCGTCTT14000.047.2547
TCGTATG14000.047.2540
CGTCTTC14050.047.08185248
TATGCCG14050.047.08185243
Produced by FastQC (version 0.11.5)