##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779736_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	163024
Sequences flagged as poor quality	0
Sequence length	76
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.54101236627736	34.0	34.0	34.0	34.0	34.0
2	33.55555010305231	34.0	34.0	34.0	34.0	34.0
3	33.56988541564432	34.0	34.0	34.0	34.0	34.0
4	33.61259078417902	34.0	34.0	34.0	34.0	34.0
5	33.612406762194524	34.0	34.0	34.0	34.0	34.0
6	37.14840759642752	38.0	38.0	38.0	36.0	38.0
7	37.149241829423886	38.0	38.0	38.0	36.0	38.0
8	36.99104426342134	38.0	37.0	38.0	35.0	38.0
9	36.99405608990087	38.0	37.0	38.0	35.0	38.0
10-11	36.901983756992834	38.0	37.0	38.0	35.0	38.0
12-13	36.87215072627343	38.0	37.0	38.0	34.5	38.0
14-15	36.903339385611936	38.0	37.0	38.0	35.0	38.0
16-17	36.88702583668662	38.0	37.0	38.0	34.5	38.0
18-19	36.88650444106389	38.0	37.0	38.0	34.5	38.0
20-21	36.9023303317303	38.0	37.0	38.0	35.0	38.0
22-23	36.822035405829816	38.0	37.0	38.0	34.0	38.0
24-25	36.833545980959855	38.0	37.0	38.0	34.0	38.0
26-27	36.73907522818726	38.0	37.0	38.0	34.0	38.0
28-29	36.69366780351359	38.0	37.0	38.0	34.0	38.0
30-31	36.606113210324864	38.0	37.0	38.0	34.0	38.0
32-33	36.60588318284424	38.0	37.0	38.0	34.0	38.0
34-35	36.57796398076357	38.0	37.0	38.0	34.0	38.0
36-37	36.64005913239768	38.0	37.0	38.0	34.0	38.0
38-39	36.30318848758465	38.0	37.0	38.0	34.0	38.0
40-41	36.5371601727353	38.0	37.0	38.0	34.0	38.0
42-43	36.66204975954461	38.0	37.0	38.0	34.0	38.0
44-45	36.647398542545886	38.0	37.0	38.0	34.0	38.0
46-47	36.680264868976344	38.0	37.0	38.0	34.0	38.0
48-49	36.670876680734125	38.0	37.0	38.0	34.0	38.0
50-51	36.63087030130533	38.0	37.0	38.0	34.0	38.0
52-53	36.62260158013544	38.0	37.0	38.0	34.0	38.0
54-55	36.61660859750711	38.0	37.0	38.0	34.0	38.0
56-57	36.638847654333105	38.0	37.0	38.0	34.0	38.0
58-59	36.586152959073516	38.0	37.0	38.0	34.0	38.0
60-61	36.650775345961335	38.0	37.0	38.0	34.0	38.0
62-63	36.640709343409554	38.0	37.0	38.0	34.0	38.0
64-65	36.64090870055943	38.0	37.0	38.0	34.0	38.0
66-67	36.61058494454804	38.0	37.0	38.0	34.0	38.0
68-69	36.484505348905685	38.0	37.0	38.0	34.0	38.0
70-71	35.95225856315635	38.0	37.0	38.0	32.5	38.0
72-73	35.20742344685445	38.0	37.0	38.0	26.0	38.0
74-75	35.12560727254883	38.0	37.0	38.0	25.0	38.0
76	34.571615222298554	38.0	36.0	38.0	24.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	1.0
21	4.0
22	9.0
23	19.0
24	39.0
25	95.0
26	151.0
27	324.0
28	569.0
29	1045.0
30	1718.0
31	2653.0
32	4190.0
33	6769.0
34	10648.0
35	18225.0
36	26330.0
37	90235.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.46412798115615	18.58622043380116	11.19283050348415	30.756821081558545
2	20.530105996663067	18.481941309255077	36.22411424084797	24.76383845323388
3	18.35741976641476	21.431813720679163	32.816027088036115	27.394739424869957
4	13.639709490627148	21.15823437039945	35.48741289626067	29.71464324271273
5	13.048998920404358	33.94469525959368	38.31460398468937	14.691701835312593
6	28.00998625969183	39.746295024045544	17.353886544312495	14.889832171950143
7	27.787933065070174	28.13512120914712	20.730689959760525	23.346255766022182
8	23.95107468838944	37.46687604279125	18.070958877220534	20.51109039159878
9	23.276940818529788	16.68956718029247	19.053022867798607	40.98046913337913
10-11	24.19889096084012	26.338759937187163	27.275738541564433	22.186610560408283
12-13	24.03388458141133	21.40267690646776	27.224519089213857	27.338919422907054
14-15	22.223721660614387	21.83328835018157	22.224948473844343	33.7180415153597
16-17	25.56280056924134	27.583668662282857	22.920858278535675	23.93267248994013
18-19	25.247202865835703	24.968716262636175	24.128349200117775	25.655731671410344
20-21	21.762439886151732	24.769359112768672	23.732088526842674	29.73611247423692
22-23	22.584098046913336	27.681507017371676	23.898628422808912	25.835766512906076
24-25	25.307623417410934	28.021640985376383	23.771959956816175	22.898775640396508
26-27	24.770279222691137	28.46360045146727	24.67366768083227	22.092452645009324
28-29	22.17464913141623	24.784694278143096	28.21823780547649	24.822418784964174
30-31	24.530130532927664	23.86918490529002	29.159510256158605	22.44117430562371
32-33	21.962103739326725	25.236161546766123	27.771677789773285	25.03005692413387
34-35	21.898616154676613	30.909866031995286	25.533357051722444	21.658160761605654
36-37	25.361296496221414	28.373122975758168	24.123441947197957	22.142138580822458
38-39	21.618289331632152	25.29842231818628	28.17744626558053	24.90584208460104
40-41	21.352377564039653	23.170208067523802	29.270843556776917	26.206570811659635
42-43	24.616620865639415	23.225721366179215	28.973034645205615	23.184623122975758
44-45	20.953356560997154	26.547624889586807	29.511605653155364	22.987412896260672
46-47	23.985425458828146	22.87945333202473	27.145696339189325	25.9894248699578
48-49	23.581190499558346	22.76413288840907	26.744528412994406	26.91014819903818
50-51	20.57335116301894	22.92423201491805	30.140347433506726	26.362069388556286
52-53	19.102708803611737	23.046913337913434	33.57787810383747	24.27249975463735
54-55	18.695100107959565	23.312825105505937	30.959245264500936	27.032829522033563
56-57	22.083864952399644	23.08034399842968	27.555758661301404	27.28003238786927
58-59	19.157301992344685	23.583950829325744	32.42958092060065	24.829166257728925
60-61	22.020684071057023	26.85432819707528	26.971182157228384	24.153805574639318
62-63	18.41538669153008	31.35151879477868	25.17206055550103	25.061033958190205
64-65	18.719022965943665	32.23298410050054	23.917644027873198	25.1303489056826
66-67	18.544202080675237	31.922293650014723	23.539478849739915	25.99402541957013
68-69	18.366620865639415	32.10723574443027	23.521996761213074	26.004146628717244
70-71	19.06805132986554	31.054936696437334	23.735462263225045	26.141549710472077
72-73	19.539454313475314	28.794226616939838	23.92899205025027	27.73732701933458
74-75	19.594047502208266	28.232039454313473	24.431985474531356	27.741927568946906
76	19.76886838747669	27.959686917263717	24.665079988222594	27.606364707037002
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	1.5
14	3.0
15	4.5
16	7.5
17	9.0
18	13.0
19	21.5
20	38.0
21	50.0
22	54.0
23	98.5
24	185.0
25	231.0
26	302.5
27	398.0
28	519.5
29	617.0
30	782.5
31	1035.5
32	1614.5
33	2106.0
34	2392.0
35	3080.5
36	3938.5
37	4394.0
38	5002.5
39	8155.0
40	11689.5
41	12487.0
42	12294.0
43	11727.5
44	11294.0
45	10923.5
46	10420.0
47	10435.5
48	9772.5
49	8613.0
50	8132.0
51	7702.5
52	6281.5
53	4936.5
54	4583.0
55	4035.0
56	3118.0
57	2357.0
58	1965.0
59	1720.5
60	1469.5
61	1366.0
62	1269.0
63	1223.0
64	1129.0
65	1111.5
66	1206.5
67	1271.0
68	1268.5
69	1281.5
70	1217.5
71	1138.0
72	1171.0
73	1153.0
74	1038.0
75	974.0
76	881.5
77	729.0
78	629.5
79	590.0
80	499.5
81	347.0
82	223.5
83	162.0
84	138.0
85	85.5
86	50.0
87	43.0
88	34.5
89	17.0
90	6.0
91	3.0
92	2.0
93	1.5
94	1.5
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	163024.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.943762881538913
#Duplication Level	Percentage of deduplicated	Percentage of total
1	51.48436899915508	16.44604475414663
2	20.276134879791073	12.953920895082932
3	9.85098701897227	9.440327804495045
4	4.9638989169675085	6.342624398861517
5	2.8573623166141795	4.563745215428403
6	1.8857055073354327	3.614191775444106
7	1.4651662954143945	3.276204730591815
8	1.059989246485905	2.708803611738149
9	0.892925723941931	2.567106683678477
>10	5.144404332129964	28.29399352242615
>50	0.09025270758122744	1.763544018058691
>100	0.021122974114755357	1.3047158700559427
>500	0.003840540748137338	0.8323927765237019
>1k	0.001920270374068669	0.6845617823142605
>5k	0.001920270374068669	5.207822161154186
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT	8490	5.207822161154186	No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG	1116	0.6845617823142605	No Hit
ATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTCTT	701	0.4299980370988321	Illumina PCR Primer Index 9 (95% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC	656	0.40239473942487	No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC	393	0.2410687996859358	No Hit
CTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTC	392	0.24045539307095887	No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGACCTCGTATGCCGT	259	0.1588723132790264	No Hit
CTTTACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTC	251	0.1539650603592109	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0018402198449308078	0.0
5	0.0	0.0	0.0	0.0030670330748846794	0.0
6	0.0	0.0	0.0	0.0036804396898616155	0.0
7	0.0	0.0	0.0	0.004293846304838552	0.0
8	0.0	0.0	0.0	0.004293846304838552	0.0
9	0.0	0.0	0.0	0.006747472764746295	0.0
10	0.0	0.0	0.0	0.008587692609677103	0.0
11	0.0	0.0	0.0	0.008587692609677103	0.0
12	0.0	0.0	0.0	0.008587692609677103	0.0
13	0.0	0.0	0.0	0.00920109922465404	0.0
14	0.0	0.0	0.0	0.010427912454607911	0.0
15	0.0	0.0	0.0	0.011654725684561782	0.0
16	0.0	0.0	0.0	0.011654725684561782	0.0
17	0.0	0.0	0.0	0.012268132299538718	0.0
18	0.0	0.0	0.0	0.01349494552949259	0.0
19	0.0	0.0	0.0	0.01349494552949259	0.0
20	0.0	0.0	0.0	0.015335165374423398	0.0
21	0.0	0.0	0.0	0.01656197860437727	0.0
22	0.0	0.0	0.0	0.02269604475414663	0.0
23	0.0	0.0	0.0	0.025763077829031308	0.0
24	0.0	0.0	0.0	0.026376484444008245	0.0
25	0.0	0.0	0.0	0.028216704288939052	0.0
26	0.0	0.0	0.0	0.03005692413386986	0.0
27	0.0	0.0	0.0	0.0631808813426244	0.0
28	0.0	0.0	0.0	0.09446461870644814	0.0
29	0.0	0.0	0.0	0.12881538914515653	0.0
30	0.0	0.0	0.0	0.15764550004907252	0.0
31	0.0	0.0	0.0	0.1981303366375503	0.0
32	0.0	0.0	0.0	0.2373883599960742	0.0
33	0.0	0.0	0.0	0.287074295809206	0.0
34	0.0	0.0	0.0	0.3741780351359309	0.0
35	0.0	0.0	0.0	0.4845912258317794	0.0
36	0.0	0.0	0.0	0.6876288153891451	0.0
37	0.0	0.0	0.0	1.06303366375503	0.0
38	0.0	0.0	0.0	1.5279958779075473	0.0
39	0.0	0.0	0.0	2.0561389734026894	0.0
40	0.0	0.0	0.0	2.727819216802434	0.0
41	0.0	0.0	0.0	3.5283148493473355	0.0
42	0.0	0.0	0.0	4.4453577387378544	0.0
43	0.0	0.0	0.0	5.417607223476298	0.0
44	0.0	0.0	0.0	6.300912749043086	0.0
45	0.0	0.0	0.0	7.232677397193052	0.0
46	0.0	0.0	0.0	8.185297870252233	0.0
47	0.0	0.0	0.0	9.079031308273628	0.0
48	0.0	0.0	0.0	9.9813524389047	0.0
49	0.0	0.0	0.0	10.880606536460888	0.0
50	0.0	0.0	0.0	11.776793600942193	0.0
51	0.0	0.0	0.0	12.733707920306212	0.0
52	0.0	0.0	0.0	13.739694768868388	0.0
53	0.0	0.0	0.0	14.80027480616351	0.0
54	0.0	0.0	0.0	15.768843851212091	0.0
55	0.0	0.0	0.0	16.668711355383255	0.0
56	0.0	0.0	0.0	17.480248306997744	0.0
57	0.0	0.0	0.0	18.236578663264304	0.0
58	0.0	0.0	0.0	19.012538031210127	0.0
59	0.0	0.0	0.0	19.778069486701344	0.0
60	0.0	0.0	0.0	20.58224555893611	0.0
61	0.0	0.0	0.0	21.322013936598292	0.0
62	0.0	0.0	0.0	22.12680341544803	0.0
63	0.0	0.0	0.0	22.972691137501226	0.0
64	0.0	0.0	0.0	23.83698105800373	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTAGAG	15	0.0022214714	70.0	18
TTAATAC	45	2.6844646E-8	54.444447	3
ATGGACA	20	0.0069413926	52.5	8
GAACGTA	20	0.0069413926	52.5	1
GCGATAA	20	0.0069413926	52.5	11
GGTGCAA	20	0.0069413926	52.5	23
TAGGACG	20	0.0069413926	52.5	4
CAATACG	20	0.0069413926	52.5	69
ACCCAGA	20	0.0069413926	52.5	12
GAGGCAC	20	0.0069413926	52.5	22
CTATAAC	55	2.2664608E-9	50.90909	4
ATCTCGT	1330	0.0	47.894737	37
TCTCGTA	1340	0.0	47.537315	38
TCTTCTG	1395	0.0	47.419353	50
CTCGTAT	1385	0.0	47.256313	39
CGTATGC	1400	0.0	47.25	41
CCGTCTT	1400	0.0	47.25	47
TCGTATG	1400	0.0	47.25	40
CGTCTTC	1405	0.0	47.081852	48
TATGCCG	1405	0.0	47.081852	43
>>END_MODULE