FastQCFastQC Report
Wed 25 May 2016
SRR1779735_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779735_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43387
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTCCG207.843006E-570.014
TGATACA207.843006E-570.02
CCAGCGC150.002210451370.066
GTGGTAG150.002210451370.048
TCTCGGT600.064.16666443
TCGGTGG600.064.16666445
ATCTCGG501.2732926E-1163.00000442
GATCTCG453.7107384E-1062.22222541
CTCGGTG750.060.6666744
GGTGGTC700.060.047
AGATCTC509.458745E-1056.00000440
AGAATGG252.3653737E-456.00000457
CGGTGGT653.6379788E-1253.84615346
GTGGTCG653.6379788E-1253.84615348
TGGACTG200.00690722152.55
TCTTACG200.00690722152.511
CCCAGCG200.00690722152.565
TCGTGTC200.00690722152.534
GAGAATG200.00690722152.556
GTGTCGA200.00690722152.536
Produced by FastQC (version 0.11.5)