FastQCFastQC Report
Wed 25 May 2016
SRR1779735_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779735_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43387
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGT609514.047986724133956No Hit
TCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCG8341.9222347707838754No Hit
ATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTCTT6351.4635720377071473RNA PCR Primer, Index 30 (95% over 22bp)
CTCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCC3960.9127157904441423No Hit
TCTTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCC3030.6983658699610482No Hit
CTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTC2900.6684029778505082No Hit
CTTTACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTC2620.6038675179201143No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGACCTCGTATGCCGT2120.4886256251872681No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGAACTCGTATGCCGT1300.29962892110540024No Hit
TCTTAATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCC1290.29732408325074333No Hit
CTTAATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCG1230.2834950561228018No Hit
TATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTCT1170.2696660289948602RNA PCR Primer, Index 30 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGAGCTCGTATGCCGT930.214349920483094No Hit
TTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTC920.2120450826284371No Hit
CTTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCG860.19821605550049554No Hit
TCTTATACACATCTCCGAGCCCACGAGACATTGCCGGACCTCGTATGCCG820.18899670408186786No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATATCGTATGCCGT600.13829027127941548No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTACAC252.7951155E-670.000012
ATCCTCG150.002210451370.037
TACCAAC150.002210451370.09
TAGGATA150.002210451370.04
CTATACA401.0353688E-861.251
TCTTAAT308.245432E-658.3333321
TCTTTAT700.055.01
ACTCTAC200.00690722152.510
AAACAAG200.00690722152.568
TGCTATC200.00690722152.52
AAGATTT200.00690722152.536
CGTTTGG200.00690722152.567
TTTATAC750.051.3333363
CTTTACA352.0540985E-550.01
TTACACA352.0540985E-550.03
CTTTATA800.048.1252
CGGACCT305.8166776E-446.66666434
ATCTCGT10200.042.20588337
TCTCGTA10200.042.20588338
GGATCTC10150.042.06896635
Produced by FastQC (version 0.11.5)