##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779735_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	43387
Sequences flagged as poor quality	0
Sequence length	76
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.44363519026437	34.0	34.0	34.0	34.0	34.0
2	33.470624841542396	34.0	34.0	34.0	34.0	34.0
3	33.51688293728536	34.0	34.0	34.0	34.0	34.0
4	33.550049554013874	34.0	34.0	34.0	34.0	34.0
5	33.55265402078964	34.0	34.0	34.0	34.0	34.0
6	37.04289303247516	38.0	37.0	38.0	35.0	38.0
7	37.05709083365985	38.0	37.0	38.0	35.0	38.0
8	36.86279300251228	38.0	37.0	38.0	35.0	38.0
9	36.841634591006525	38.0	37.0	38.0	34.0	38.0
10-11	36.7423421762279	38.0	37.0	38.0	34.0	38.0
12-13	36.72386890082282	38.0	37.0	38.0	34.0	38.0
14-15	36.75188420494618	38.0	37.0	38.0	34.0	38.0
16-17	36.630995459469425	38.0	37.0	38.0	34.0	38.0
18-19	36.6362274413995	38.0	37.0	38.0	34.0	38.0
20-21	36.71728858874778	38.0	37.0	38.0	34.0	38.0
22-23	36.62389655887708	38.0	37.0	38.0	34.0	38.0
24-25	36.57944776085003	38.0	37.0	38.0	34.0	38.0
26-27	36.377590061539166	38.0	37.0	38.0	34.0	38.0
28-29	36.29445456012169	38.0	37.0	38.0	34.0	38.0
30-31	36.356915665982896	38.0	37.0	38.0	34.0	38.0
32-33	36.026885933574576	38.0	37.0	38.0	32.5	38.0
34-35	35.86722981538249	38.0	36.5	38.0	31.5	38.0
36-37	35.79569917256321	38.0	36.0	38.0	31.0	38.0
38-39	34.51864613824417	38.0	34.0	38.0	24.5	38.0
40-41	35.676562103856	38.0	36.5	38.0	31.5	38.0
42-43	36.214799363864756	38.0	37.0	38.0	34.0	38.0
44-45	36.21138820383986	38.0	37.0	38.0	34.0	38.0
46-47	36.244209094890174	38.0	37.0	38.0	34.0	38.0
48-49	36.194562887500865	38.0	37.0	38.0	33.5	38.0
50-51	36.00507064328025	38.0	37.0	38.0	32.0	38.0
52-53	36.037453615138176	38.0	37.0	38.0	33.5	38.0
54-55	36.03262497983267	38.0	37.0	38.0	33.0	38.0
56-57	36.04558969276512	38.0	37.0	38.0	32.5	38.0
58-59	35.8777053034319	38.0	37.0	38.0	32.0	38.0
60-61	35.94095005416369	38.0	37.0	38.0	32.0	38.0
62-63	36.030469956438566	38.0	37.0	38.0	32.5	38.0
64-65	36.11291400649964	38.0	37.0	38.0	34.0	38.0
66-67	36.022679604489824	38.0	37.0	38.0	32.0	38.0
68-69	35.60689837969899	38.0	36.0	38.0	30.5	38.0
70-71	34.11261437757854	38.0	34.5	38.0	24.5	38.0
72-73	31.591409869315694	38.0	26.5	38.0	11.0	38.0
74-75	31.312167239034736	38.0	25.0	38.0	11.0	38.0
76	30.805471685066955	37.0	25.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	2.0
21	2.0
22	8.0
23	11.0
24	22.0
25	59.0
26	128.0
27	203.0
28	355.0
29	613.0
30	883.0
31	1202.0
32	1843.0
33	2791.0
34	4442.0
35	7029.0
36	6873.0
37	16921.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.875607900984164	15.894161845714155	12.512964712932446	40.71726554036923
2	15.944868278516605	15.412450734090857	45.81556687487035	22.827114112522185
3	16.643234148477653	18.219743241062993	41.752137737110196	23.38488487334916
4	13.105308041579274	29.594118053794915	30.149583976767236	27.150989927858575
5	12.86790974254961	30.62438057482656	42.37905363357688	14.12865604904695
6	25.173439048562933	43.73429829211515	15.064420218037661	16.027842441284257
7	22.68882384124277	26.397307949385763	17.645838615253417	33.268029594118055
8	19.807776522921614	43.6351902643649	15.901076359278123	20.655956853435363
9	19.43669762832185	16.394311660174708	15.43319427478277	48.735796436720676
10-11	21.671237928411735	28.28727499020444	27.361882591559684	22.67960448982414
12-13	21.675847604121053	16.09929241477862	29.60679466199553	32.6180653191048
14-15	21.074284924055593	16.88524212321663	18.359185931269735	43.68128702145804
16-17	30.914790144513333	26.99080369695992	16.448475349759146	25.645930808767602
18-19	31.119920713577798	20.645585083089404	16.9163574342545	31.318136769078297
20-21	21.61592181989997	18.293498052412012	16.919814691036485	43.17076543665153
22-23	21.724249199068847	28.290732246986423	16.627100283495054	33.35791827044967
24-25	31.108396524304517	28.63761034411229	16.831078433632193	23.422914697951
26-27	30.79263373821652	30.59672252057068	17.839444995044598	20.771198746168206
28-29	21.479936386475213	29.667872865143934	19.38138151981008	29.470809228570772
30-31	20.89681240924701	19.858482955724067	36.492497752783095	22.752206882245833
32-33	31.025422361536865	17.33122824809275	21.61015972526333	30.033189665107056
34-35	31.065757023993363	18.290040795630027	18.276211768502083	32.36799041187452
36-37	32.55122502131975	26.148385461082814	19.047180030884828	22.25320948671261
38-39	21.120381681148732	19.982944199875536	28.479728951068296	30.41694516790744
40-41	19.760527346901146	17.47067093829949	30.650886210155114	32.11791550464425
42-43	29.726646230437687	17.59858943923295	29.908728420955587	22.766035909373773
44-45	20.327517459146748	27.024223845852447	31.298545647313713	21.349713047687093
46-47	29.142369834282157	18.219743241062993	22.558600502454652	30.079286422200198
48-49	28.590361168091828	16.799963122594324	20.57298269066771	34.03669301864613
50-51	20.66287136699933	17.214833936432573	30.904418374167374	31.217876322400716
52-53	18.44446493189204	17.084610597644456	40.50867771452278	23.96224675594072
54-55	18.457141540092657	17.107658976191026	32.855463618134465	31.579735865581853
56-57	28.322999976951625	17.35888630234863	23.522022725701248	30.796090994998504
58-59	20.20766589070459	19.10480097725125	37.49971189526817	23.187821236775992
60-61	27.58084218775209	29.624080945905458	21.815290294327795	20.979786572014657
62-63	18.30156498490331	42.71210270357481	18.069928780510292	20.916403531011593
64-65	17.490262060064072	43.75504183280706	17.773757116186875	20.98093899094199
66-67	17.65851522345403	43.83455873879273	17.154908152211494	21.352017885541752
68-69	17.417659667642383	43.79998617097287	17.574388641759054	21.207965519625695
70-71	17.74840389978565	41.1736234355913	18.295802890266668	22.782169774356372
72-73	18.727959988014845	35.17874017562864	20.25837232350704	25.83492751284947
74-75	18.904280083896097	32.188213059211286	21.552538778896903	27.35496807799571
76	19.466660520432388	32.70564915758176	21.0016825316339	26.826007790351948
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	3.0
16	8.0
17	10.0
18	15.5
19	27.0
20	37.5
21	42.0
22	48.5
23	81.5
24	121.0
25	134.0
26	159.5
27	208.0
28	258.0
29	285.0
30	332.0
31	405.5
32	484.5
33	537.0
34	595.0
35	714.5
36	808.0
37	840.0
38	889.5
39	1015.0
40	1230.5
41	2600.0
42	3830.0
43	4282.0
44	4358.0
45	3358.5
46	2735.0
47	2290.5
48	1611.5
49	1236.0
50	1095.0
51	1001.5
52	821.0
53	728.0
54	722.0
55	677.5
56	625.5
57	598.0
58	578.0
59	574.5
60	615.5
61	690.5
62	721.0
63	702.5
64	719.0
65	755.0
66	766.5
67	777.0
68	786.0
69	788.0
70	792.5
71	804.0
72	777.0
73	753.5
74	692.5
75	628.0
76	578.0
77	508.5
78	397.0
79	305.0
80	278.5
81	222.0
82	156.0
83	120.0
84	92.0
85	53.0
86	32.5
87	23.0
88	16.0
89	6.0
90	3.0
91	1.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	43387.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.93401249222117
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.1061655126937	32.34839929010994
2	21.153030945315813	20.7020536105285
3	7.790495030851114	11.436605434807662
4	2.722434176440111	5.328785119966811
5	1.1257124016767932	2.754281236315025
6	0.32499646742970184	0.9542028718279669
7	0.26376524892845365	0.9034964390255146
8	0.11775234327163112	0.46096757093138496
9	0.08007159342470915	0.3526401917625095
>10	0.23550468654326223	1.8507847972895106
>50	0.023550468654326223	0.9518980339733099
>100	0.0423908435777872	4.522091870836887
>500	0.009420187461730487	3.385806808491023
>1k	0.0	0.0
>5k	0.004710093730865244	14.047986724133956
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGT	6095	14.047986724133956	No Hit
TCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCG	834	1.9222347707838754	No Hit
ATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTCTT	635	1.4635720377071473	RNA PCR Primer, Index 30 (95% over 22bp)
CTCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCC	396	0.9127157904441423	No Hit
TCTTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCC	303	0.6983658699610482	No Hit
CTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTC	290	0.6684029778505082	No Hit
CTTTACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTC	262	0.6038675179201143	No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGACCTCGTATGCCGT	212	0.4886256251872681	No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGAACTCGTATGCCGT	130	0.29962892110540024	No Hit
TCTTAATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCC	129	0.29732408325074333	No Hit
CTTAATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCG	123	0.2834950561228018	No Hit
TATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTCT	117	0.2696660289948602	RNA PCR Primer, Index 30 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGAGCTCGTATGCCGT	93	0.214349920483094	No Hit
TTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTC	92	0.2120450826284371	No Hit
CTTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCG	86	0.19821605550049554	No Hit
TCTTATACACATCTCCGAGCCCACGAGACATTGCCGGACCTCGTATGCCG	82	0.18899670408186786	No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATATCGTATGCCGT	60	0.13829027127941548	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.006914513563970774	0.0
2	0.0	0.0	0.0	0.006914513563970774	0.0
3	0.0	0.0	0.0	0.006914513563970774	0.0
4	0.0	0.0	0.0	0.011524189273284625	0.0
5	0.0	0.0	0.0	0.011524189273284625	0.0
6	0.0	0.0	0.0	0.011524189273284625	0.0
7	0.0	0.0	0.0	0.013829027127941549	0.0
8	0.0	0.0	0.0	0.013829027127941549	0.0
9	0.0	0.0	0.0	0.016133864982598473	0.0
10	0.0	0.0	0.0	0.016133864982598473	0.0
11	0.0	0.0	0.0	0.018438702837255398	0.0
12	0.0	0.0	0.0	0.018438702837255398	0.0
13	0.0	0.0	0.0	0.020743540691912324	0.0
14	0.0	0.0	0.0	0.020743540691912324	0.0
15	0.0	0.0	0.0	0.020743540691912324	0.0
16	0.0	0.0	0.0	0.020743540691912324	0.0
17	0.0	0.0	0.0	0.020743540691912324	0.0
18	0.0	0.0	0.0	0.02304837854656925	0.0
19	0.0	0.0	0.0	0.02304837854656925	0.0
20	0.0	0.0	0.0	0.025353216401226175	0.0
21	0.0	0.0	0.0	0.025353216401226175	0.0
22	0.0	0.0	0.0	0.029962892110540023	0.0
23	0.0	0.0	0.0	0.032267729965196945	0.0
24	0.0	0.0	0.0	0.03457256781985387	0.0
25	0.0	0.0	0.0	0.03457256781985387	0.0
26	0.0	0.0	0.0	0.04148708138382465	0.0
27	0.0	0.0	0.0	0.059925784221080046	0.0
28	0.0	0.0	0.0	0.11524189273284624	0.0
29	0.0	0.0	0.0	0.17747251480858323	0.0
30	0.0	0.0	0.0	0.2235692719017217	0.0
31	0.0	0.0	0.0	0.2650563532855464	0.0
32	0.0	0.0	0.0	0.3434208403438818	0.0
33	0.0	0.0	0.0	0.4356143545301588	0.0
34	0.0	0.0	0.0	0.583123977228202	0.0
35	0.0	0.0	0.0	0.7490723027635006	0.0
36	0.0	0.0	0.0	1.1178463595086086	0.0
37	0.0	0.0	0.0	1.4635720377071473	0.0
38	0.0	0.0	0.0	1.8553944729988245	0.0
39	0.0	0.0	0.0	2.2564362597091296	0.0
40	0.0	0.0	0.0	2.6851361006753174	0.0
41	0.0	0.0	0.0	3.0838730495309656	0.0
42	0.0	0.0	0.0	3.588632539700832	0.0
43	0.0	0.0	0.0	3.982759812847166	0.0
44	0.0	0.0	0.0	4.43681287021458	0.0
45	0.0	0.0	0.0	4.971535252494987	0.0
46	0.0	0.0	0.0	5.556964067577846	0.0
47	0.0	0.0	0.0	6.151612234079333	0.0
48	0.0	0.0	0.0	6.534215317952382	0.0
49	0.0	0.0	0.0	6.997487726738424	0.0
50	0.0	0.0	0.0	7.426187567704612	0.0
51	0.0	0.0	0.0	7.859497084380114	0.0
52	0.0	0.0	0.0	8.33429368243944	0.0
53	0.0	0.0	0.0	8.70998225274852	0.0
54	0.0	0.0	0.0	9.090280498766912	0.0
55	0.0	0.0	0.0	9.525894853297071	0.0
56	0.0	0.0	0.0	9.910802775024777	0.0
57	0.0	0.0	0.0	10.316454237444395	0.0
58	0.0	0.0	0.0	10.71058151059073	0.0
59	0.0	0.0	0.0	11.04017332380667	0.0
60	0.0	0.0	0.0	11.51957959757531	0.0
61	0.0	0.0	0.0	11.888353654320419	0.0
62	0.0	0.0	0.0	12.204116440408418	0.0
63	0.0	0.0	0.0	12.568280821444212	0.0
64	0.0	0.0	0.0	12.994675824555742	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTACAC	25	2.7951155E-6	70.00001	2
ATCCTCG	15	0.0022104513	70.0	37
TACCAAC	15	0.0022104513	70.0	9
TAGGATA	15	0.0022104513	70.0	4
CTATACA	40	1.0353688E-8	61.25	1
TCTTAAT	30	8.245432E-6	58.333332	1
TCTTTAT	70	0.0	55.0	1
ACTCTAC	20	0.006907221	52.5	10
AAACAAG	20	0.006907221	52.5	68
TGCTATC	20	0.006907221	52.5	2
AAGATTT	20	0.006907221	52.5	36
CGTTTGG	20	0.006907221	52.5	67
TTTATAC	75	0.0	51.333336	3
CTTTACA	35	2.0540985E-5	50.0	1
TTACACA	35	2.0540985E-5	50.0	3
CTTTATA	80	0.0	48.125	2
CGGACCT	30	5.8166776E-4	46.666664	34
ATCTCGT	1020	0.0	42.205883	37
TCTCGTA	1020	0.0	42.205883	38
GGATCTC	1015	0.0	42.068966	35
>>END_MODULE