FastQCFastQC Report
Wed 25 May 2016
SRR1779734_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779734_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences108406
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCGGA150.002212152870.0581766
TTCGGAA150.002212152870.0581767
CGTGTAC207.899927E-569.99354635
GCCGTAT553.45608E-1157.2674454
GTCTATC252.3824409E-455.99483538
ACGTGTA252.3824409E-455.99483534
GTAGATC850.053.52447538
TCGGAAT200.006912387452.5436368
CGGAATA200.00693767952.49515569
TTAGGCC200.00693767952.4951553
CGAGTAA200.00693767952.49515510
GACAACT200.00693767952.49515515
AGAACTT200.00693767952.49515526
CCGTATC608.367351E-1152.49515555
GTGTACT200.00693767952.49515536
TGAATTG200.00693767952.49515532
GAATTAT200.00693767952.49515537
TCGGTGG1000.052.49515545
ATATTGA200.00693767952.49515528
TTCCGAC552.2555469E-950.90439219
Produced by FastQC (version 0.11.5)