##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779734_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	108406
Sequences flagged as poor quality	0
Sequence length	76
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.029407966348725	34.0	23.0	34.0	12.0	34.0
2	27.495747467852333	33.0	23.0	34.0	12.0	34.0
3	26.412191207128757	32.0	21.0	34.0	11.0	34.0
4	28.79122926775271	33.0	23.0	34.0	12.0	34.0
5	28.39601129088796	34.0	23.0	34.0	11.0	34.0
6	30.769514602512775	36.0	26.0	38.0	11.0	38.0
7	31.567136505359482	37.0	27.0	38.0	12.0	38.0
8	30.903058871280187	36.0	26.0	38.0	11.0	38.0
9	32.377792742099146	37.0	31.0	38.0	12.0	38.0
10-11	32.062699481578505	37.0	31.0	38.0	11.0	38.0
12-13	31.107544785343983	35.5	27.0	38.0	11.0	38.0
14-15	31.148183679870115	36.5	26.5	38.0	11.0	38.0
16-17	30.793355533826542	35.5	26.5	38.0	11.0	38.0
18-19	31.330848846004834	36.5	27.5	38.0	11.5	38.0
20-21	30.206303156651845	35.0	24.5	38.0	11.0	38.0
22-23	30.21049572901869	35.5	26.0	38.0	11.0	38.0
24-25	31.45415382912385	36.5	28.5	38.0	11.0	38.0
26-27	29.992366658672026	35.0	24.0	38.0	11.0	38.0
28-29	32.16645757614892	37.0	31.0	38.0	11.0	38.0
30-31	32.66975536409424	37.0	31.5	38.0	11.0	38.0
32-33	32.774325221851186	37.0	32.5	38.0	11.0	38.0
34-35	32.87266387469329	37.0	34.0	38.0	11.0	38.0
36-37	30.603518255447117	36.0	25.0	38.0	11.0	38.0
38-39	28.767964872793016	34.0	22.0	37.5	11.0	38.0
40-41	30.026760511410806	35.0	24.5	37.5	11.0	38.0
42-43	31.141818718521115	36.0	28.0	38.0	11.0	38.0
44-45	32.878673689648174	37.0	33.5	38.0	11.0	38.0
46-47	31.416854233160528	36.5	28.5	38.0	11.0	38.0
48-49	30.78455989520875	35.5	27.0	38.0	11.0	38.0
50-51	28.508989354832757	34.0	21.5	37.5	11.0	38.0
52-53	31.974955260778927	36.5	29.0	38.0	11.0	38.0
54-55	30.999423463645925	36.5	27.0	38.0	11.0	38.0
56-57	31.474918362452264	36.5	28.5	38.0	11.0	38.0
58-59	32.62060218069111	37.0	32.0	38.0	11.0	38.0
60-61	33.14517185395643	37.5	34.0	38.0	11.0	38.0
62-63	33.01916868070033	38.0	34.0	38.0	11.0	38.0
64-65	32.70332361677398	37.5	32.5	38.0	11.0	38.0
66-67	30.477902514620965	36.5	24.5	38.0	11.0	38.0
68-69	30.57556316071066	36.0	25.0	38.0	11.0	38.0
70-71	30.06717340368614	35.0	24.0	38.0	11.0	38.0
72-73	28.81227515082191	34.0	22.0	37.5	11.0	38.0
74-75	31.086240613988156	35.5	28.0	37.5	11.0	38.0
76	31.421019131782373	36.0	27.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	0.0
8	0.0
9	0.0
10	0.0
11	8.0
12	30.0
13	179.0
14	463.0
15	816.0
16	1350.0
17	2085.0
18	2690.0
19	2962.0
20	2625.0
21	2119.0
22	1831.0
23	1651.0
24	1731.0
25	1871.0
26	2152.0
27	2478.0
28	2897.0
29	3604.0
30	4395.0
31	5515.0
32	7073.0
33	9215.0
34	12222.0
35	14618.0
36	14817.0
37	7008.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.8040698854307	19.36608674796598	11.256757006069774	29.57308636053355
2	20.627087061601756	17.749017582052655	38.42499492648008	23.198900429865503
3	19.806099293397043	17.084847702156708	37.57356603878014	25.535486965666106
4	14.538863162555579	18.46761249377341	32.5904470232275	34.40307732044352
5	14.065642123129715	29.39320701806173	39.265354316181764	17.275796542626793
6	27.424681290703468	33.951995277014184	16.758297511207868	21.86502592107448
7	27.664520413999227	25.48567422467391	19.931553604044055	26.918251757282807
8	23.90203403902034	31.702412250357455	18.27037498270375	26.125178727918453
9	23.568806154640885	16.045237348486246	19.686179731749164	40.6997767651237
10-11	24.98039758313731	23.289054932890547	26.663438033301045	25.067109450671094
12-13	24.410774410774412	19.3404363267377	29.365342926986767	26.883446335501134
14-15	23.991052073243853	22.409021724090216	23.343941700106082	30.255984502559848
16-17	25.53768524369376	22.643893934292393	25.69358283097104	26.12483799104281
18-19	22.215662489449336	22.799581202060782	27.823772779056412	27.16098352943347
20-21	25.197983497147288	22.378477106788864	26.570607579873716	25.85293181619014
22-23	25.926848392601816	21.40307181403072	27.01120797011208	25.658871823255385
24-25	22.614627486612765	24.972441435167035	24.174050209629584	28.238880868590616
26-27	23.949541072828744	23.82823670494903	26.68742216687422	25.534800055348
28-29	23.57721702312152	24.253381977851678	25.826641637186302	26.342759361840496
30-31	23.221607759753887	24.372379630184817	25.9198103417262	26.486202268335095
32-33	23.009796505728467	24.337213807353837	25.841281847868196	26.811707839049497
34-35	23.600739814861793	24.05274640124348	25.86492382766557	26.481589956229158
36-37	24.895877054208505	21.73552079922144	29.51879747798774	23.849804668582316
38-39	24.478575711452425	20.8039297080393	33.00770259674369	21.709791983764585
40-41	23.767463527805578	23.35742519913841	25.109658731879215	27.765452541176792
42-43	21.600280427282623	23.990830765824768	26.49345977159936	27.91542903529325
44-45	21.703896055975534	22.613913629046763	28.387197948424653	27.29499236655305
46-47	24.020109773534433	21.556201282228677	28.750980120843135	25.67270882339375
48-49	23.986329106918006	21.966597635728817	29.209311335679462	24.837761921673714
50-51	26.329505096628385	20.35238227019049	30.30994880309949	23.00816383008164
52-53	20.84313454176468	23.481850468151837	28.401826484018265	27.273188506065217
54-55	22.91545592915456	22.535860892025276	28.13938471472718	26.409298464092984
56-57	22.68012231851705	22.637227815931848	27.79333151915724	26.889318346393864
58-59	20.560859738941932	23.745214704118816	27.913380379133805	27.78054517780545
60-61	20.63225574348165	23.09753656410422	27.98289754671119	28.28731014570294
62-63	20.567778239011115	23.896038005627044	27.72427471057608	27.81190904478576
64-65	20.899866242331996	24.236889442368895	26.859000968590006	28.004243346709103
66-67	19.56044462893778	25.54725335547253	23.56902356902357	31.323278446566118
68-69	20.02924785946265	25.450250959551223	23.750738116327135	30.76976306465899
70-71	20.203035851832716	25.464348804708294	23.014727248407585	31.317888095051405
72-73	19.528158295281585	26.05829989391633	21.5967898159679	32.816751994834185
74-75	21.62446381624464	24.434758544347584	23.912181172455146	30.028596466952635
76	21.81910428485771	23.2784465661178	25.067109450671094	29.835339698353398
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.5
12	1.0
13	0.5
14	1.0
15	3.0
16	2.5
17	1.0
18	8.0
19	14.5
20	18.5
21	23.0
22	34.0
23	54.5
24	87.5
25	111.0
26	149.0
27	228.0
28	355.5
29	442.0
30	528.0
31	735.0
32	1034.0
33	1212.0
34	1400.5
35	1859.5
36	2450.0
37	2770.0
38	3112.0
39	3831.5
40	4483.0
41	5059.0
42	5361.0
43	5612.5
44	6024.5
45	6265.0
46	6345.0
47	6205.0
48	6051.0
49	5818.0
50	5599.0
51	5265.0
52	4672.5
53	4094.0
54	3774.0
55	3547.5
56	3123.5
57	2712.0
58	2498.0
59	2372.0
60	2143.0
61	1962.5
62	1885.0
63	1828.5
64	1742.5
65	1669.0
66	1602.5
67	1580.0
68	1539.5
69	1460.0
70	1342.0
71	1263.0
72	1190.0
73	1036.0
74	892.5
75	830.0
76	768.5
77	609.5
78	447.5
79	383.0
80	325.0
81	234.5
82	165.0
83	128.0
84	99.0
85	56.0
86	36.0
87	30.0
88	22.0
89	8.0
90	2.5
91	4.0
92	5.0
93	3.5
94	1.0
95	0.5
96	1.0
97	1.5
98	1.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	9.224581665221482E-4
9	0.0
10-11	9.224581665221482E-4
12-13	9.224581665221482E-4
14-15	9.224581665221482E-4
16-17	0.0013836872497832223
18-19	4.612290832610741E-4
20-21	4.612290832610741E-4
22-23	9.224581665221482E-4
24-25	4.612290832610741E-4
26-27	9.224581665221482E-4
28-29	4.612290832610741E-4
30-31	4.612290832610741E-4
32-33	0.0
34-35	4.612290832610741E-4
36-37	4.612290832610741E-4
38-39	9.224581665221482E-4
40-41	0.0013836872497832223
42-43	0.0
44-45	0.0013836872497832223
46-47	9.224581665221482E-4
48-49	4.612290832610741E-4
50-51	9.224581665221482E-4
52-53	9.224581665221482E-4
54-55	9.224581665221482E-4
56-57	4.612290832610741E-4
58-59	9.224581665221482E-4
60-61	4.612290832610741E-4
62-63	9.224581665221482E-4
64-65	9.224581665221482E-4
66-67	9.224581665221482E-4
68-69	0.02029407966348726
70-71	0.0013836872497832223
72-73	9.224581665221482E-4
74-75	9.224581665221482E-4
76	9.224581665221482E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	108406.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	95.94273037779244
#Duplication Level	Percentage of deduplicated	Percentage of total
1	97.04213876742229	93.10487755047116
2	2.229856084139794	4.2787696212380855
3	0.45201563726316085	1.301028432374564
4	0.15499431754826712	0.5948231207449334
5	0.058997660281648094	0.2830198306661378
6	0.03199872697180092	0.18420271405728542
7	0.010999562338368227	0.0738729630592648
8	0.006999721486496868	0.05372579130388876
9	0.001999920424694722	0.01726900434751733
>10	0.009999602123469606	0.10841097173714857
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0018449163330442964	0.0
10	0.0	0.0	0.0	0.0027673744995664446	0.0
11	0.0	0.0	0.0	0.005534748999132889	0.0
12	0.0	0.0	0.0	0.005534748999132889	0.0
13	0.0	0.0	0.0	0.005534748999132889	0.0
14	0.0	0.0	0.0	0.008302123498699333	0.0
15	0.0	0.0	0.0	0.008302123498699333	0.0
16	0.0	0.0	0.0	0.009224581665221483	0.0
17	0.0	0.0	0.0	0.01014703983174363	0.0
18	0.0	0.0	0.0	0.011069497998265778	0.0
19	0.0	0.0	0.0	0.013836872497832223	0.0
20	0.0	0.0	0.0	0.01568178883087652	0.0
21	0.0	0.0	0.0	0.02029407966348726	0.0
22	0.0	0.0	0.0	0.03689832666088593	0.0
23	0.0	0.0	0.0	0.037820784827408076	0.0
24	0.0	0.0	0.0	0.048890282825673856	0.0
25	0.0	0.0	0.0	0.0516576573252403	0.0
26	0.0	0.0	0.0	0.05442503182480674	0.0
27	0.0	0.0	0.0	0.0728741951552497	0.0
28	0.0	0.0	0.0	0.09593564931830341	0.0
29	0.0	0.0	0.0	0.11622972898179068	0.0
30	0.0	0.0	0.0	0.14667084847702155	0.0
31	0.0	0.0	0.0	0.16143017914137595	0.0
32	0.0	0.0	0.0	0.19925096396878403	0.0
33	0.0	0.0	0.0	0.22692470896444847	0.0
34	0.0	0.0	0.0	0.2619781192922901	0.0
35	0.0	0.0	0.0	0.33208493994797333	9.224581665221482E-4
36	9.224581665221482E-4	0.0	0.0	0.385587513606258	9.224581665221482E-4
37	9.224581665221482E-4	0.0	0.0	0.5885283102411306	9.224581665221482E-4
38	9.224581665221482E-4	0.0	0.0	0.796081397708614	9.224581665221482E-4
39	9.224581665221482E-4	0.0	0.0	1.039610353670461	9.224581665221482E-4
40	9.224581665221482E-4	0.0	0.0	1.4012139549471432	9.224581665221482E-4
41	9.224581665221482E-4	0.0	0.0	1.8532184565429959	9.224581665221482E-4
42	9.224581665221482E-4	0.0	0.0	2.328284412301902	9.224581665221482E-4
43	9.224581665221482E-4	0.0	0.0	2.819032156891685	9.224581665221482E-4
44	9.224581665221482E-4	0.0	0.0	3.2950205708171136	9.224581665221482E-4
45	9.224581665221482E-4	0.0	0.0	3.6003542239359443	9.224581665221482E-4
46	9.224581665221482E-4	0.0	0.0	4.000701068206557	9.224581665221482E-4
47	9.224581665221482E-4	0.0	0.0	4.532959430289837	9.224581665221482E-4
48	9.224581665221482E-4	0.0	0.0	4.889028282567385	9.224581665221482E-4
49	9.224581665221482E-4	0.0	0.0	5.26908104717451	9.224581665221482E-4
50	9.224581665221482E-4	0.0	0.0	5.7450694610999395	9.224581665221482E-4
51	9.224581665221482E-4	0.0	0.0	6.3382100621736805	9.224581665221482E-4
52	9.224581665221482E-4	0.0	0.0	6.925815914248289	9.224581665221482E-4
53	9.224581665221482E-4	0.0	0.0	7.667472280132096	9.224581665221482E-4
54	9.224581665221482E-4	0.0	0.0	8.335331992694131	9.224581665221482E-4
55	9.224581665221482E-4	0.0	0.0	8.746748334963009	9.224581665221482E-4
56	9.224581665221482E-4	0.0	0.0	9.20613250189104	9.224581665221482E-4
57	9.224581665221482E-4	0.0	0.0	9.552976772503367	9.224581665221482E-4
58	9.224581665221482E-4	0.0	0.0	9.91919266461266	9.224581665221482E-4
59	9.224581665221482E-4	0.0	0.0	10.322306883382838	9.224581665221482E-4
60	9.224581665221482E-4	0.0	0.0	10.587052377174695	9.224581665221482E-4
61	9.224581665221482E-4	0.0	0.0	10.852720329133074	9.224581665221482E-4
62	9.224581665221482E-4	0.0	0.0	11.045514085936203	9.224581665221482E-4
63	9.224581665221482E-4	0.0	0.0	11.342545615556334	9.224581665221482E-4
64	9.224581665221482E-4	0.0	0.0	11.6368097706769	9.224581665221482E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTCGGA	15	0.0022121528	70.05817	66
TTCGGAA	15	0.0022121528	70.05817	67
CGTGTAC	20	7.899927E-5	69.993546	35
GCCGTAT	55	3.45608E-11	57.26744	54
GTCTATC	25	2.3824409E-4	55.994835	38
ACGTGTA	25	2.3824409E-4	55.994835	34
GTAGATC	85	0.0	53.524475	38
TCGGAAT	20	0.0069123874	52.54363	68
CGGAATA	20	0.006937679	52.495155	69
TTAGGCC	20	0.006937679	52.495155	3
CGAGTAA	20	0.006937679	52.495155	10
GACAACT	20	0.006937679	52.495155	15
AGAACTT	20	0.006937679	52.495155	26
CCGTATC	60	8.367351E-11	52.495155	55
GTGTACT	20	0.006937679	52.495155	36
TGAATTG	20	0.006937679	52.495155	32
GAATTAT	20	0.006937679	52.495155	37
TCGGTGG	100	0.0	52.495155	45
ATATTGA	20	0.006937679	52.495155	28
TTCCGAC	55	2.2555469E-9	50.904392	19
>>END_MODULE