FastQCFastQC Report
Wed 25 May 2016
SRR1779734_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779734_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences108406
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGT92958.574248657823368No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCG12611.163219747984429No Hit
ATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTCTT7730.7130601627216205Illumina PCR Primer Index 10 (95% over 23bp)
CTCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC6810.6281940114015829No Hit
CTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTC4070.37544047377451434Illumina PCR Primer Index 10 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC3800.35053410327841633No Hit
CTTTACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTC3390.31271331845100825Illumina PCR Primer Index 10 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACAGTAGATTACCTCGTATGCCGT1870.1724996771396417No Hit
TCTTAATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC1650.15220559747615445No Hit
TTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTC1590.14667084847702155Illumina PCR Primer Index 10 (95% over 21bp)
TATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTCT1480.13652380864527794Illumina PCR Primer Index 10 (95% over 22bp)
CTTAATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCG1410.1300666014796229No Hit
CTTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCG1340.12360939431396785No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTTAT650.064.615391
TCACGCA200.00693514852.50000430
AGCCGAA200.00693514852.50000412
TTAGATT200.00693514852.50000430
CACGCAA200.00693514852.50000431
TGCGGAT200.00693514852.5000044
TACTGTA200.00693514852.50000416
GTCCGGA200.00693514852.50000434
ATCGCTC200.00693514852.50000468
TCAGTGG200.00693514852.50000470
CTTGTAC305.85574E-446.6666643
TTGTACT305.85574E-446.6666644
CCCAAAG305.85574E-446.66666461
CTTTATA900.046.6666642
CCGTCTT16000.043.96874647
TCTTCTG16050.043.83177650
CGTCTTC16100.043.69565248
TATGCCG16100.043.69565243
GTCTTCT16100.043.69565249
CGTATGC16150.043.56037541
Produced by FastQC (version 0.11.5)