##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779734_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	108406
Sequences flagged as poor quality	0
Sequence length	76
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.52134568197332	34.0	34.0	34.0	34.0	34.0
2	33.53510875781783	34.0	34.0	34.0	34.0	34.0
3	33.54715606147261	34.0	34.0	34.0	34.0	34.0
4	33.609864767632786	34.0	34.0	34.0	34.0	34.0
5	33.59672896334151	34.0	34.0	34.0	34.0	34.0
6	37.1419017397561	38.0	38.0	38.0	36.0	38.0
7	37.152030330424516	38.0	38.0	38.0	36.0	38.0
8	36.96897773185986	38.0	37.0	38.0	35.0	38.0
9	36.97849750013837	38.0	37.0	38.0	35.0	38.0
10-11	36.889909230116416	38.0	37.0	38.0	35.0	38.0
12-13	36.86242458904489	38.0	37.0	38.0	34.5	38.0
14-15	36.9006420308839	38.0	37.0	38.0	35.0	38.0
16-17	36.86419109643377	38.0	37.0	38.0	34.5	38.0
18-19	36.86275667398483	38.0	37.0	38.0	34.0	38.0
20-21	36.88751083888346	38.0	37.0	38.0	35.0	38.0
22-23	36.812473479327714	38.0	37.0	38.0	34.0	38.0
24-25	36.79723447041677	38.0	37.0	38.0	34.0	38.0
26-27	36.67183089496891	38.0	37.0	38.0	34.0	38.0
28-29	36.61061195874767	38.0	37.0	38.0	34.0	38.0
30-31	36.5010885006365	38.0	37.0	38.0	34.0	38.0
32-33	36.55121948969614	38.0	37.0	38.0	34.0	38.0
34-35	36.36938914820213	38.0	37.0	38.0	34.0	38.0
36-37	36.4306403704592	38.0	37.0	38.0	34.0	38.0
38-39	36.12303285795989	38.0	37.0	38.0	34.0	38.0
40-41	36.431336826374924	38.0	37.0	38.0	34.0	38.0
42-43	36.57647639429552	38.0	37.0	38.0	34.0	38.0
44-45	36.55412062062985	38.0	37.0	38.0	34.0	38.0
46-47	36.58000479678247	38.0	37.0	38.0	34.0	38.0
48-49	36.571661162666274	38.0	37.0	38.0	34.0	38.0
50-51	36.49454827223585	38.0	37.0	38.0	34.0	38.0
52-53	36.47663874693283	38.0	37.0	38.0	34.0	38.0
54-55	36.469042303931516	38.0	37.0	38.0	34.0	38.0
56-57	36.50981956718263	38.0	37.0	38.0	34.0	38.0
58-59	36.42271645480877	38.0	37.0	38.0	34.0	38.0
60-61	36.48737615999114	38.0	37.0	38.0	34.0	38.0
62-63	36.51070973931333	38.0	37.0	38.0	34.0	38.0
64-65	36.53938896371049	38.0	37.0	38.0	34.0	38.0
66-67	36.48419367931665	38.0	37.0	38.0	34.0	38.0
68-69	36.29099404092024	38.0	37.0	38.0	34.0	38.0
70-71	35.437415825692305	38.0	36.5	38.0	29.0	38.0
72-73	34.12304669483239	38.0	36.0	38.0	21.5	38.0
74-75	33.93879028836042	38.0	36.0	38.0	21.0	38.0
76	33.385670534841246	38.0	34.0	38.0	19.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	0.0
20	1.0
21	2.0
22	9.0
23	23.0
24	36.0
25	87.0
26	159.0
27	295.0
28	485.0
29	870.0
30	1385.0
31	2006.0
32	3182.0
33	4978.0
34	7885.0
35	13643.0
36	18120.0
37	55239.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.47763038946184	17.055329040828	11.122078113757542	34.34496245595262
2	19.50353301477778	17.015663339667547	38.60026197811929	24.88054166743538
3	18.448240872276443	19.46940206261646	35.021124292013354	27.061232773093742
4	13.607180414368209	23.396306477501245	33.08488460048336	29.911628507647176
5	13.953102226814016	31.812814788849327	39.00245373872295	15.231629245613712
6	28.439385273877832	40.04667638322602	16.24541077062155	15.268527572274598
7	27.130417135582903	27.455122410198697	18.30618231463203	27.108278139586368
8	23.586332859804806	38.92219987823552	16.50646643174732	20.98500083021235
9	22.21187019168681	16.369942623102045	17.10790915631976	44.31027802889139
10-11	23.825249524934044	26.401213954947146	26.835230522295817	22.938305997823
12-13	23.620002582882865	19.320425068723132	27.80058299356124	29.25898935483276
14-15	22.232625500433556	19.77427448665203	20.200911388668523	37.792188624245895
16-17	27.78951349556298	26.779883032304486	20.748390310499417	24.682213161633122
18-19	27.629467003671383	23.318819991513383	21.32031437374315	27.73139863107208
20-21	21.96372894489235	22.073501466708485	21.193476375846355	34.76929321255281
22-23	22.679095253030276	27.18069110565836	21.266350571001606	28.873863070309763
24-25	27.80750142981016	27.724941423906422	20.947180045384943	23.520377100898475
26-27	27.784439975647103	28.630334114347917	21.310167333911405	22.275058576093574
28-29	22.31472427725403	28.530247403280264	22.278748408759665	26.876279910706046
30-31	27.514159732856115	23.67350515654115	26.91179455011715	21.90054056048558
32-33	26.744829621976645	28.111912624762468	23.948397690164754	21.194860063096137
34-35	22.897256609412764	27.982307252366105	27.792742099145805	21.32769403907533
36-37	21.356751471320777	26.982823828939356	29.36368835673302	22.296736343006845
38-39	21.086471228529785	23.01579248381086	28.711049203918606	27.18668708374075
40-41	21.145969780270466	21.16165156910134	29.305573492242125	28.386805158386068
42-43	26.57555854841983	21.23452576425659	28.802833791487558	23.387081895836022
44-45	21.39641717248123	26.233787797723373	29.454550486135457	22.915244543659945
46-47	26.38322602069996	20.926885965721453	24.837186133608842	27.852701879969743
48-49	25.869878051030387	20.52607789236758	24.157795693965276	29.446248362636755
50-51	21.16488017268417	20.67966717709352	29.617364352526614	28.5380882976957
52-53	19.70601258232939	20.788056011659872	35.28771470213826	24.218216703872482
54-55	19.38269099496338	20.901979595225356	31.08822389904618	28.627105510765087
56-57	24.60887773739461	20.708724609338965	25.82836743353689	28.854030219729538
58-59	20.038097522277365	21.46929136763648	33.98889360367507	24.503717506411085
60-61	24.58489382506503	27.05293064959504	24.98155083666956	23.380624688670366
62-63	18.930686493367528	34.929800933527666	22.537959153552386	23.601553419552424
64-65	18.76187664889397	35.737413058317806	21.502038632548015	23.99867166024021
66-67	18.72682323856613	35.43669169603159	21.232219618840286	24.604265446562
68-69	18.641957087246094	35.79414423555892	21.231297160673762	24.332601516521226
70-71	19.183440030994596	34.267475969964764	21.50895706879693	25.040126930243712
72-73	19.951386454624284	31.1684777595336	22.07119532129218	26.808940464549934
74-75	20.10082467760087	30.487242403556998	22.51628138663912	26.89565153220301
76	20.31068391048466	30.69756286552405	22.501522055974764	26.490231168016532
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	3.5
15	4.5
16	11.0
17	20.0
18	22.5
19	24.0
20	23.5
21	24.0
22	38.0
23	72.0
24	101.5
25	111.0
26	149.5
27	224.0
28	365.0
29	470.0
30	536.5
31	662.0
32	931.0
33	1141.0
34	1305.0
35	1725.5
36	2297.0
37	2612.0
38	4717.0
39	7738.5
40	8290.5
41	7322.0
42	6718.0
43	6273.5
44	5760.5
45	5572.0
46	5452.0
47	5483.0
48	5318.5
49	4849.5
50	4576.0
51	4308.5
52	3561.5
53	2961.5
54	2841.0
55	2661.5
56	2272.0
57	1883.0
58	1704.0
59	1600.5
60	1467.0
61	1360.0
62	1283.0
63	1300.5
64	1281.0
65	1292.0
66	1363.0
67	1386.0
68	1363.5
69	1282.5
70	1231.5
71	1239.0
72	1226.0
73	1193.5
74	1077.0
75	980.0
76	922.0
77	780.0
78	634.5
79	573.0
80	517.0
81	395.5
82	260.0
83	190.0
84	163.5
85	110.5
86	61.5
87	39.0
88	28.0
89	13.5
90	6.5
91	3.0
92	3.0
93	2.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	108406.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.11678320388171
#Duplication Level	Percentage of deduplicated	Percentage of total
1	54.901239393869716	22.02461118388281
2	22.451654441352986	18.013763075844512
3	9.935845846076019	11.957825212626608
4	4.686242498102969	7.519878973488553
5	2.389109889857205	4.79217017508256
6	1.4072524086550622	3.387266387469328
7	0.926671112235278	2.60225448775898
8	0.6875301800455288	2.2065199343209785
9	0.4920784566212146	1.7766544287216575
>10	2.0763871323783025	12.361861889563308
>50	0.016096024282002346	0.37820784827408077
>100	0.020694888362574444	1.9002638230356255
>500	0.004598864080572099	1.3412541741232036
>1k	0.0022994320402860494	1.163219747984429
>5k	0.0022994320402860494	8.574248657823368
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGT	9295	8.574248657823368	No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCG	1261	1.163219747984429	No Hit
ATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTCTT	773	0.7130601627216205	Illumina PCR Primer Index 10 (95% over 23bp)
CTCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC	681	0.6281940114015829	No Hit
CTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTC	407	0.37544047377451434	Illumina PCR Primer Index 10 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC	380	0.35053410327841633	No Hit
CTTTACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTC	339	0.31271331845100825	Illumina PCR Primer Index 10 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACAGTAGATTACCTCGTATGCCGT	187	0.1724996771396417	No Hit
TCTTAATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC	165	0.15220559747615445	No Hit
TTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTC	159	0.14667084847702155	Illumina PCR Primer Index 10 (95% over 21bp)
TATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTCT	148	0.13652380864527794	Illumina PCR Primer Index 10 (95% over 22bp)
CTTAATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCG	141	0.1300666014796229	No Hit
CTTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCG	134	0.12360939431396785	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	9.224581665221482E-4	0.0
4	0.0	0.0	0.0	0.0027673744995664446	0.0
5	0.0	0.0	0.0	0.004612290832610741	0.0
6	0.0	0.0	0.0	0.004612290832610741	0.0
7	0.0	0.0	0.0	0.004612290832610741	0.0
8	0.0	0.0	0.0	0.004612290832610741	0.0
9	0.0	0.0	0.0	0.007379665332177186	0.0
10	0.0	0.0	0.0	0.011991956164787926	0.0
11	0.0	0.0	0.0	0.016604246997398667	0.0
12	0.0	0.0	0.0	0.016604246997398667	0.0
13	0.0	0.0	0.0	0.016604246997398667	0.0
14	0.0	0.0	0.0	0.017526705163920814	0.0
15	0.0	0.0	0.0	0.019371621496965113	0.0
16	0.0	0.0	0.0	0.019371621496965113	0.0
17	0.0	0.0	0.0	0.022138995996531557	0.0
18	0.0	0.0	0.0	0.023983912329575852	0.0
19	0.0	0.0	0.0	0.0267512868291423	0.0
20	0.0	0.0	0.0	0.03136357766175304	0.0
21	0.0	0.0	0.0	0.03597586849436378	0.0
22	0.0	0.0	0.0	0.03874324299393023	0.0
23	0.0	0.0	0.0	0.04151061749349667	0.0
24	0.0	0.0	0.0	0.04612290832610741	0.0
25	0.0	0.0	0.0	0.05073519915871815	0.0
26	0.0	0.0	0.0	0.055347489991328894	0.0
27	0.0	0.0	0.0	0.0802538604874269	0.0
28	0.0	0.0	0.0	0.11346235448222423	0.0
29	0.0	0.0	0.0	0.14667084847702155	0.0
30	0.0	0.0	0.0	0.1872590078039961	0.0
31	0.0	0.0	0.0	0.24352895596184712	0.0
32	0.0	0.0	0.0	0.3062561112853532	0.0
33	0.0	0.0	0.0	0.37082818294190356	0.0
34	0.0	0.0	0.0	0.4750659557589063	0.0
35	0.0	0.0	0.0	0.5894507684076528	0.0
36	0.0	0.0	0.0	0.7887017323764367	0.0
37	0.0	0.0	0.0	1.1604523734848624	0.0
38	0.0	0.0	0.0	1.5368153054258988	0.0
39	0.0	0.0	0.0	1.9620685201926094	0.0
40	0.0	0.0	0.0	2.546906997767651	0.0
41	0.0	0.0	0.0	3.2138442521631645	0.0
42	0.0	0.0	0.0	3.9222921240521744	0.0
43	0.0	0.0	0.0	4.636274744940317	0.0
44	0.0	0.0	0.0	5.3078242901684405	0.0
45	0.0	0.0	0.0	5.956312381233511	0.0
46	0.0	0.0	0.0	6.638008966293379	0.0
47	0.0	0.0	0.0	7.431322989502426	0.0
48	0.0	0.0	0.0	8.09826024389794	0.0
49	0.0	0.0	0.0	8.81962253011826	0.0
50	0.0	0.0	0.0	9.441359334354187	0.0
51	0.0	0.0	0.0	10.159031787908418	0.0
52	0.0	0.0	0.0	10.961570392782688	0.0
53	0.0	0.0	0.0	11.785325535486965	0.0
54	0.0	0.0	0.0	12.537128941202516	0.0
55	0.0	0.0	0.0	13.220670442595429	0.0
56	0.0	0.0	0.0	13.89590982048964	0.0
57	0.0	0.0	0.0	14.49643008689556	0.0
58	0.0	0.0	0.0	15.142150803461064	0.0
59	0.0	0.0	0.0	15.743593528033504	0.0
60	0.0	0.0	0.0	16.32935446377507	0.0
61	0.0	0.0	0.0	16.9344870210136	0.0
62	0.0	0.0	0.0	17.511945833256462	0.0
63	0.0	0.0	0.0	18.15951146615501	0.0
64	0.0	0.0	0.0	18.8172241388853	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTTTAT	65	0.0	64.61539	1
TCACGCA	20	0.006935148	52.500004	30
AGCCGAA	20	0.006935148	52.500004	12
TTAGATT	20	0.006935148	52.500004	30
CACGCAA	20	0.006935148	52.500004	31
TGCGGAT	20	0.006935148	52.500004	4
TACTGTA	20	0.006935148	52.500004	16
GTCCGGA	20	0.006935148	52.500004	34
ATCGCTC	20	0.006935148	52.500004	68
TCAGTGG	20	0.006935148	52.500004	70
CTTGTAC	30	5.85574E-4	46.666664	3
TTGTACT	30	5.85574E-4	46.666664	4
CCCAAAG	30	5.85574E-4	46.666664	61
CTTTATA	90	0.0	46.666664	2
CCGTCTT	1600	0.0	43.968746	47
TCTTCTG	1605	0.0	43.831776	50
CGTCTTC	1610	0.0	43.695652	48
TATGCCG	1610	0.0	43.695652	43
GTCTTCT	1610	0.0	43.695652	49
CGTATGC	1615	0.0	43.560375	41
>>END_MODULE