FastQCFastQC Report
Wed 25 May 2016
SRR1779733_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779733_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences151226
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA452.6833732E-854.44264253
AACGAGC200.00693225452.51563366
ACGGGGT200.00694133152.49826444
ATACGCT200.00694133152.4982644
GCCGTAT552.2646418E-950.9074154
CCGTATC703.8016879E-1044.99851255
TAGATCT1100.044.54398339
ATCTCGG950.044.20906442
TCTCGGT1300.043.07549743
GTAGATC855.638867E-1141.1751138
CGCAGAG602.5805457E-740.8319870
GATCTCG1050.039.99867641
TCGGTGG1150.039.56390845
CGTTTAG459.1658214E-538.88760435
AGATCTC1200.037.91541340
CGTATCA654.834128E-737.6910656
CTCGGTG1550.036.1278444
CGACGAT501.7038117E-434.99884422
TTCCGCT501.7038117E-434.99884413
TATCTAT400.002413207734.9988443