##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779733_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	151226
Sequences flagged as poor quality	0
Sequence length	76
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.571065822014734	34.0	27.0	34.0	12.0	34.0
2	27.874022985465462	34.0	23.0	34.0	12.0	34.0
3	26.759380000793513	32.0	21.0	34.0	11.0	34.0
4	29.22873712192348	34.0	23.0	34.0	12.0	34.0
5	28.73661275177549	34.0	23.0	34.0	11.0	34.0
6	31.10633753455094	36.0	27.0	38.0	11.0	38.0
7	32.07290413024215	37.0	28.0	38.0	12.0	38.0
8	31.264418816870116	37.0	27.0	38.0	11.0	38.0
9	32.82233213865341	37.0	31.0	38.0	21.0	38.0
10-11	32.50023144168331	37.0	31.0	38.0	11.0	38.0
12-13	31.46538293679658	36.0	29.0	38.0	16.0	38.0
14-15	31.58419517807784	36.5	29.0	38.0	11.5	38.0
16-17	31.221049951727878	36.0	27.5	38.0	11.0	38.0
18-19	31.734536389245235	37.0	29.0	38.0	11.5	38.0
20-21	30.615320778173064	35.5	26.5	38.0	11.0	38.0
22-23	30.581110391070318	35.5	26.0	38.0	11.0	38.0
24-25	31.914171504899947	36.5	29.0	38.0	11.0	38.0
26-27	30.385171861981405	35.5	25.0	38.0	11.0	38.0
28-29	32.65745308346449	37.0	31.5	38.0	11.0	38.0
30-31	33.18116593707431	37.0	34.0	38.0	21.0	38.0
32-33	33.28337719704284	38.0	34.0	38.0	21.0	38.0
34-35	33.343525584224935	38.0	34.0	38.0	21.0	38.0
36-37	31.031132212714745	36.5	27.0	38.0	11.0	38.0
38-39	29.071770065993945	34.0	22.5	37.5	11.0	38.0
40-41	30.44163702008914	35.0	25.5	37.5	11.0	38.0
42-43	31.56943911761205	36.5	28.5	38.0	16.0	38.0
44-45	33.35133508788171	37.0	34.0	38.0	21.0	38.0
46-47	31.814664806316372	37.0	29.0	38.0	16.0	38.0
48-49	31.16965006017484	36.0	27.0	38.0	16.0	38.0
50-51	28.80186938753918	34.0	21.5	37.5	11.0	38.0
52-53	32.42787946517133	36.5	32.5	38.0	16.0	38.0
54-55	31.41681324639943	36.5	28.0	38.0	15.0	38.0
56-57	31.862011162101755	36.5	29.0	38.0	16.0	38.0
58-59	33.05658749156891	37.0	33.5	38.0	21.0	38.0
60-61	33.55599896843135	38.0	34.0	38.0	21.0	38.0
62-63	33.43187348736328	38.0	34.0	38.0	20.0	38.0
64-65	33.125305833652945	37.5	33.0	38.0	16.0	38.0
66-67	30.86550262520995	36.5	25.5	38.0	11.0	38.0
68-69	30.975143163212675	36.5	26.0	38.0	11.0	38.0
70-71	30.47871728406491	35.5	25.5	38.0	11.0	38.0
72-73	29.155621387856584	34.0	22.5	37.5	11.0	38.0
74-75	31.46880827370955	36.0	28.0	38.0	14.5	38.0
76	31.85392723473477	37.0	29.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	1.0
5	1.0
6	0.0
7	0.0
8	0.0
9	0.0
10	2.0
11	3.0
12	30.0
13	156.0
14	468.0
15	831.0
16	1359.0
17	2251.0
18	2901.0
19	3279.0
20	3037.0
21	2771.0
22	2462.0
23	2464.0
24	2565.0
25	2838.0
26	3134.0
27	3659.0
28	4314.0
29	5195.0
30	6507.0
31	7976.0
32	10090.0
33	12991.0
34	17028.0
35	20556.0
36	21784.0
37	10573.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.83292555512941	18.258765027177866	11.26393609564493	29.644373322047795
2	20.776189279621228	17.56642376310952	37.98420906457884	23.67317789269041
3	21.084337349397593	16.691574200203668	36.48380569478793	25.740282755610806
4	15.329374578445506	18.21710552418235	31.402668853239526	35.05085104413263
5	15.12570589713409	29.16165209686165	37.95974237234338	17.752899633660878
6	29.46980016663801	31.97267665613056	16.288865671247006	22.26865750598442
7	27.782920926295745	26.664065702987582	19.570708740560487	25.98230463015619
8	24.16894255503462	31.5520787181844	17.92386078837214	26.35511793840884
9	23.631987197714633	15.653042195962255	19.677562275579774	41.03740833074334
10-11	25.813949643736876	23.462447717766864	25.991833225875776	24.731769412620476
12-13	25.100596137505082	19.62922429384322	28.71312990375173	26.557049664899967
14-15	23.969488869124177	22.783826587798853	23.168687406388557	30.07799713668841
16-17	25.320142969088387	23.242726728672842	25.351223205694957	26.08590709654381
18-19	22.790184032852146	23.037170271717926	27.268669448430465	26.903976246999466
20-21	24.89659345273717	22.558001897853178	26.74948007419482	25.79592457521483
22-23	25.33956699422042	22.08772533097036	26.716350795519173	25.85635687929005
24-25	23.765738242606993	24.596624874358568	23.650015870496745	27.987621012537694
26-27	24.47412795503389	23.2640105802612	26.448338568358405	25.8135228963465
28-29	23.86129185843517	24.504708247368143	25.067780246521714	26.56621964767497
30-31	23.53610231247892	24.676140534178	25.183999788391976	26.603757364951097
32-33	23.72985590816212	24.273424016188013	25.339730067516186	26.656990008133686
34-35	23.475848661087728	24.477346444170383	25.643501175412553	26.403303719329337
36-37	24.455355519185307	22.1732877052026	29.1967134520326	24.174643323579495
38-39	24.04767809023128	21.11160706905388	32.2713220585561	22.56939278215874
40-41	23.59701763957083	23.377804228868058	24.884524458992544	28.140653672568565
42-43	21.971307559290988	23.61786615264061	26.01562577492404	28.395200513144363
44-45	22.43350030584073	22.639157532774554	27.88969895352874	27.03764320785597
46-47	23.569088137756367	21.62858285914557	28.894649310457698	25.90767969264036
48-49	23.387976696666513	22.212560258690807	29.077918041567752	25.32154500307493
50-51	25.08712299055038	21.025902144515054	30.780370710804572	23.106604154129993
52-53	20.814762402295962	23.702900371639046	28.311687452883838	27.17064977318115
54-55	22.784052584941346	22.784052584941346	27.755220801206175	26.676674028911133
56-57	22.30737947475095	23.09561675268725	27.448776149209614	27.148227623352188
58-59	20.659555755407577	23.79962042810948	27.997394576221872	27.54342924026107
60-61	20.7994154328376	23.267955271354225	27.976564411498252	27.956064884309928
62-63	21.065248011215225	24.12033883734617	27.259080959906896	27.55533219153171
64-65	21.30356723657368	24.186386375133495	26.35040188859536	28.159644499697468
66-67	20.159698457570798	24.451057217014665	23.616855957281487	31.772388368133047
68-69	20.54295739480986	24.676815011525193	23.722720673587293	31.05750692007765
70-71	20.610757693986326	24.6409252621973	23.228432370951317	31.51988467286506
72-73	20.095752313628505	24.898577271389698	21.803158900567706	33.2025115144141
74-75	22.25554313827923	24.577610548659926	23.504691746625845	29.662154566435
76	22.531113197815134	23.455581859782306	24.15653806985756	29.856766872545
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	1.0
6	1.0
7	0.0
8	0.0
9	0.5
10	0.5
11	0.0
12	0.0
13	0.5
14	4.5
15	9.0
16	12.5
17	15.0
18	26.5
19	55.0
20	91.5
21	111.0
22	141.5
23	232.5
24	350.5
25	408.0
26	501.0
27	682.5
28	919.5
29	1068.0
30	1241.0
31	1688.5
32	2181.5
33	2400.0
34	2649.5
35	3242.0
36	3879.5
37	4174.0
38	4501.0
39	5051.0
40	5638.5
41	6129.5
42	6256.0
43	6376.0
44	6557.5
45	6725.0
46	6831.0
47	6934.5
48	6840.0
49	6570.0
50	6498.0
51	6317.0
52	5964.0
53	5597.5
54	5403.0
55	5278.5
56	4920.5
57	4563.5
58	4440.0
59	4285.5
60	3994.5
61	3667.5
62	3477.0
63	3359.0
64	3123.5
65	2868.5
66	2655.5
67	2580.0
68	2387.0
69	2134.5
70	1996.0
71	1917.0
72	1806.0
73	1584.5
74	1300.5
75	1127.0
76	1059.0
77	876.0
78	668.5
79	576.0
80	533.0
81	389.0
82	241.5
83	195.0
84	162.5
85	105.0
86	60.0
87	40.0
88	37.5
89	26.0
90	12.5
91	5.5
92	3.0
93	2.5
94	2.5
95	2.0
96	1.0
97	0.5
98	1.0
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.001983785856929364
9	0.001983785856929364
10-11	0.002314416833084258
12-13	0.00165315488077447
14-15	0.00165315488077447
16-17	0.002975678785394046
18-19	0.001983785856929364
20-21	0.00165315488077447
22-23	0.002645047809239152
24-25	0.001322523904619576
26-27	6.61261952309788E-4
28-29	0.001322523904619576
30-31	0.001983785856929364
32-33	0.001983785856929364
34-35	0.00165315488077447
36-37	0.002314416833084258
38-39	0.002314416833084258
40-41	0.002314416833084258
42-43	9.91892928464682E-4
44-45	0.002314416833084258
46-47	0.00165315488077447
48-49	0.001983785856929364
50-51	0.001983785856929364
52-53	0.002645047809239152
54-55	0.002645047809239152
56-57	0.00165315488077447
58-59	0.001983785856929364
60-61	0.001983785856929364
62-63	0.001983785856929364
64-65	0.00165315488077447
66-67	0.002314416833084258
68-69	0.023474799306997474
70-71	0.002645047809239152
72-73	0.00165315488077447
74-75	0.001983785856929364
76	0.002645047809239152
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	151226.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	97.4531607790878
#Duplication Level	Percentage of deduplicated	Percentage of total
1	97.62253798125286	95.13624889549641
2	2.1647018511056606	4.219140750691779
3	0.19258341209807534	0.5630358666773718
4	0.01817630203305322	0.07085352337585585
5	0.001001575821892923	0.004880336480168904
6	9.988776884740657E-4	0.0058406272784104025
7	0.0	0.0
8	0.0	0.0
9	0.0	0.0
>10	0.0	0.0
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.001983785856929364	0.0
5	0.0	0.0	0.0	0.002645047809239152	0.0
6	0.0	0.0	0.0	0.002645047809239152	0.0
7	0.0	0.0	0.0	0.003967571713858728	0.0
8	0.0	0.0	0.0	0.003967571713858728	0.0
9	0.0	0.0	0.0	0.005951357570788092	0.0
10	0.0	0.0	0.0	0.007273881475407668	0.0
11	0.0	0.0	0.0	0.007935143427717456	0.0
12	0.0	0.0	0.0	0.008596405380027244	0.0
13	0.0	0.0	0.0	0.009257667332337032	0.0
14	0.0	0.0	0.0	0.010580191236956608	0.0
15	0.0	0.0	0.0	0.010580191236956608	0.0
16	0.0	0.0	0.0	0.011241453189266396	0.0
17	0.0	0.0	0.0	0.011241453189266396	0.0
18	0.0	0.0	0.0	0.011241453189266396	0.0
19	0.0	0.0	0.0	0.011902715141576184	0.0
20	0.0	0.0	0.0	0.013886500998505549	0.0
21	0.0	0.0	0.0	0.01587028685543491	0.0
22	0.0	0.0	0.0	0.022482906378532792	0.0
23	0.0	0.0	0.0	0.025127954187771945	0.0
24	0.0	0.0	0.0	0.028434263949320884	0.0
25	0.0	0.0	0.0	0.033724359567799185	0.0
26	0.0	0.0	0.0	0.03835319323396771	0.0
27	0.0	0.0	0.0	0.08530279184796265	0.0
28	0.0	0.0	0.0	0.11043074603573459	0.0
29	0.0	0.0	0.0	0.13489743827119674	0.0
30	0.0	0.0	0.0	0.16134791636358828	0.0
31	0.0	0.0	0.0	0.1719281076005449	0.0
32	0.0	0.0	0.0	0.20102363350217556	0.0
33	0.0	0.0	0.0	0.22152275402377897	0.0
34	0.0	0.0	0.0	0.2539245896869586	0.0
35	0.0	0.0	0.0	0.3260021424887255	0.0
36	0.0	0.0	0.0	0.4033697909089707	0.0
37	0.0	0.0	0.0	0.6321664264081573	0.0
38	0.0	0.0	0.0	0.87352703900123	0.0
39	0.0	0.0	0.0	1.1367092960205256	0.0
40	0.0	0.0	0.0	1.5109835610278657	0.0
41	0.0	0.0	0.0	1.993704786214011	0.0
42	0.0	0.0	0.0	2.5961144247682277	0.0
43	0.0	0.0	0.0	3.228280851176385	0.0
44	0.0	0.0	0.0	3.814820202875167	0.0
45	0.0	0.0	0.0	4.184465634216338	0.0
46	0.0	0.0	0.0	4.6123021173607714	0.0
47	0.0	0.0	0.0	5.074524222025313	0.0
48	0.0	0.0	0.0	5.403171412323278	0.0
49	0.0	0.0	0.0	5.665692407390264	0.0
50	0.0	0.0	0.0	6.121963154484018	0.0
51	0.0	0.0	0.0	6.70453493446894	0.0
52	0.0	0.0	0.0	7.29967069154775	0.0
53	0.0	0.0	0.0	8.152037348075066	0.0
54	0.0	0.0	0.0	8.79875153743404	0.0
55	0.0	0.0	0.0	9.272215095287848	0.0
56	0.0	0.0	0.0	9.749646224855514	0.0
57	0.0	0.0	0.0	10.145080872336768	0.0
58	0.0	0.0	0.0	10.44529379868541	0.0
59	0.0	0.0	0.0	10.809649134408104	0.0
60	0.0	0.0	0.0	11.04307460357346	0.0
61	0.0	0.0	0.0	11.29038657373732	0.0
62	0.0	0.0	0.0	11.4510732281486	0.0
63	0.0	0.0	0.0	11.751947416449552	0.0
64	0.0	0.0	0.0	12.013145887611918	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCCGTA	45	2.6833732E-8	54.442642	53
AACGAGC	20	0.006932254	52.515633	66
ACGGGGT	20	0.006941331	52.498264	44
ATACGCT	20	0.006941331	52.498264	4
GCCGTAT	55	2.2646418E-9	50.90741	54
CCGTATC	70	3.8016879E-10	44.998512	55
TAGATCT	110	0.0	44.543983	39
ATCTCGG	95	0.0	44.209064	42
TCTCGGT	130	0.0	43.075497	43
GTAGATC	85	5.638867E-11	41.17511	38
CGCAGAG	60	2.5805457E-7	40.83198	70
GATCTCG	105	0.0	39.998676	41
TCGGTGG	115	0.0	39.563908	45
CGTTTAG	45	9.1658214E-5	38.887604	35
AGATCTC	120	0.0	37.915413	40
CGTATCA	65	4.834128E-7	37.69106	56
CTCGGTG	155	0.0	36.12784	44
CGACGAT	50	1.7038117E-4	34.998844	22
TTCCGCT	50	1.7038117E-4	34.998844	13
TATCTAT	40	0.0024132077	34.998844	3
>>END_MODULE