FastQCFastQC Report
Wed 25 May 2016
SRR1779733_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779733_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences151226
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT100036.61460330895481No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG12290.8126909393887294No Hit
ATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTCTT7740.5118167510877759RNA PCR Primer, Index 24 (95% over 23bp)
CTCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC7390.4886725827569333No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC4560.30153545025326334No Hit
CTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTC4380.28963273511168713No Hit
CTTTACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTC2910.1924272281221483RNA PCR Primer, Index 24 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCACCTCGTATGCCGT2280.15076772512663167No Hit
CTTAATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG1600.10580191236956608No Hit
TATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTCT1560.10315686456032692RNA PCR Primer, Index 24 (95% over 22bp)
CTTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG1540.10183434065570734No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGTCT150.002221159270.023
CGAGATA150.002221159270.027
GCATAAC252.384379E-455.99999611
CGATTCT200.00694042552.546
TCGAGAT200.00694042552.526
TCTCGTA16050.048.8473538
CATCTCG16000.048.78124636
ATCTCGT16150.048.54489537
TATGCCG16750.048.26865843
CTCGTAT16700.048.20359439
CGTATGC16900.048.04733741
TCGTATG16950.047.9056140
ATGCCGT16800.047.70833244
CCGTCTT16700.047.57485247
TGCTTGA16850.047.56676555
GTATGCC17050.047.41935742
TGCCGTC16950.047.28613745
CTTGAAA16850.047.15133757
CGTCTTC16800.047.08333248
GCCGTCT16950.047.07964746
Produced by FastQC (version 0.11.5)