##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779733_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	151226
Sequences flagged as poor quality	0
Sequence length	76
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.553522542419955	34.0	34.0	34.0	34.0	34.0
2	33.56635102429476	34.0	34.0	34.0	34.0	34.0
3	33.581685688968825	34.0	34.0	34.0	34.0	34.0
4	33.638944361419334	34.0	34.0	34.0	34.0	34.0
5	33.62231362331874	34.0	34.0	34.0	34.0	34.0
6	37.20026979487654	38.0	38.0	38.0	36.0	38.0
7	37.19618319601127	38.0	38.0	38.0	36.0	38.0
8	37.033010196659305	38.0	37.0	38.0	35.0	38.0
9	37.03865076111251	38.0	37.0	38.0	36.0	38.0
10-11	36.9578147937524	38.0	37.0	38.0	35.0	38.0
12-13	36.933787840715226	38.0	37.0	38.0	35.0	38.0
14-15	36.96726092074114	38.0	37.0	38.0	35.0	38.0
16-17	36.93522277915173	38.0	37.0	38.0	35.0	38.0
18-19	36.947862801370135	38.0	37.0	38.0	35.0	38.0
20-21	36.957097324534146	38.0	37.0	38.0	35.0	38.0
22-23	36.879022125824925	38.0	37.0	38.0	35.0	38.0
24-25	36.87063732426964	38.0	37.0	38.0	35.0	38.0
26-27	36.77986920238583	38.0	37.0	38.0	34.5	38.0
28-29	36.69809424305344	38.0	37.0	38.0	34.0	38.0
30-31	36.60064737545131	38.0	37.0	38.0	34.0	38.0
32-33	36.64337151019005	38.0	37.0	38.0	34.0	38.0
34-35	36.61036792615026	38.0	37.0	38.0	34.0	38.0
36-37	36.64735230714295	38.0	37.0	38.0	34.0	38.0
38-39	36.260328911695076	38.0	37.0	38.0	34.0	38.0
40-41	36.509158478039495	38.0	37.0	38.0	34.0	38.0
42-43	36.6326524539431	38.0	37.0	38.0	34.0	38.0
44-45	36.61654080647508	38.0	37.0	38.0	34.0	38.0
46-47	36.653277214235644	38.0	37.0	38.0	34.0	38.0
48-49	36.636041421448695	38.0	37.0	38.0	34.0	38.0
50-51	36.567015592556835	38.0	37.0	38.0	34.0	38.0
52-53	36.560326927909216	38.0	37.0	38.0	34.0	38.0
54-55	36.5604757118485	38.0	37.0	38.0	34.0	38.0
56-57	36.58057146257919	38.0	37.0	38.0	34.0	38.0
58-59	36.50921137899567	38.0	37.0	38.0	34.0	38.0
60-61	36.567862007855794	38.0	37.0	38.0	34.0	38.0
62-63	36.56423167973761	38.0	37.0	38.0	34.0	38.0
64-65	36.569207675928745	38.0	37.0	38.0	34.0	38.0
66-67	36.53321518786452	38.0	37.0	38.0	34.0	38.0
68-69	36.384599870392655	38.0	37.0	38.0	34.0	38.0
70-71	35.73739965349874	38.0	37.0	38.0	31.0	38.0
72-73	34.75467181569307	38.0	37.0	38.0	23.5	38.0
74-75	34.6430739423115	38.0	37.0	38.0	23.0	38.0
76	34.08392075436763	38.0	35.0	38.0	22.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	0.0
19	0.0
20	2.0
21	8.0
22	14.0
23	24.0
24	55.0
25	129.0
26	233.0
27	365.0
28	592.0
29	1075.0
30	1700.0
31	2551.0
32	4078.0
33	6090.0
34	10054.0
35	17436.0
36	23899.0
37	82920.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.37616547419094	17.011624985121607	11.373044317776044	32.2391652229114
2	20.103685874122174	18.6191527911867	36.75756814304418	24.519593191646937
3	20.08847684921905	19.24206155026252	33.732955973179216	26.936505627339212
4	14.695224366180417	21.559123431156017	31.84571436128708	31.899937841376484
5	15.111158134183276	31.209580363165063	37.30773808736593	16.371523415285733
6	30.75066456826207	37.27401372779813	16.16058085249891	15.81474085144089
7	27.560075648367345	27.63281446312142	18.40490391863833	26.402205969872906
8	24.32716596352479	38.00603070900507	17.562456191395658	20.104347136074484
9	23.413963207384974	16.292833242960867	17.766786134659384	42.526417414994775
10-11	24.87965032467962	26.00247311970164	26.24085805350932	22.877018502109426
12-13	24.747067303241508	19.784626982132703	26.872032586989008	28.596273127636785
14-15	22.91933926705725	20.91736870643937	20.739489241268036	35.423802785235345
16-17	27.104466163225897	26.614140425588197	21.138560829486995	25.142832581698915
18-19	27.0333805033526	23.81237353365162	22.211788978085778	26.942456984910002
20-21	23.22583418195284	22.55233888352532	21.841151653816144	32.3806752807057
22-23	23.22120534828667	26.925594805126103	21.602105458056155	28.251094388531072
24-25	27.534617063203413	26.99039847645246	21.57631624191607	23.89866821842805
26-27	27.381865552219857	27.243000542234803	22.25444037400976	23.120693531535583
28-29	27.065451708039625	23.280718923994552	22.91305727851031	26.74077208945552
30-31	27.36268895560287	22.697816513033473	26.72953063626625	23.209963895097406
32-33	23.398423551505694	27.015195799664077	26.658114345416795	22.928266303413434
34-35	26.178368799016045	27.171584251385344	23.834525809054	22.815521140544615
36-37	23.31973337918083	26.50172589369553	23.064155634613094	27.114385092510545
38-39	22.370161215663973	22.97521590202743	27.231428458069377	27.423194424239217
40-41	22.081851004456908	21.812386758890668	28.171412323277746	27.934349913374685
42-43	26.441220425059182	21.14021398436777	28.298043987145068	24.12052160342798
44-45	22.43198920820494	25.25524711359158	29.025101503709678	23.287662174493803
46-47	25.199701109597555	21.54788197796675	25.96147487865843	27.29094203377726
48-49	25.1114226389642	21.144842818033936	25.239707457712296	28.504027085289568
50-51	21.33297184346607	21.64839379471784	29.43409202121328	27.58454234060281
52-53	20.04549482231891	21.703609167735706	33.67840186211365	24.572494147831723
54-55	20.112943541454513	21.84015976088768	30.075185483977624	27.97171121368019
56-57	23.944956555089732	21.508206260828164	25.99718302408316	28.549654159998944
58-59	20.167167021543914	22.01803922605901	32.62104400037031	25.19374975202677
60-61	23.397101027601074	26.430640233822228	25.6113366749104	24.5609220636663
62-63	19.83918109319826	32.02161004060148	23.285678388636875	24.853530477563382
64-65	19.651052067766127	33.047227328633966	22.266343089151334	25.035377514448577
66-67	19.728750347162524	32.84785685001256	21.919841826141006	25.503550976683904
68-69	19.655350270456136	32.92985333209898	21.968444579635776	25.446351817809106
70-71	19.96482086413712	31.623860976287144	22.346025154404668	26.06529300517107
72-73	20.93092457646172	29.351764908150713	22.83006890349543	26.887241611892136
74-75	21.55251081163292	28.676616454842417	23.045309668972266	26.725563064552393
76	21.664925343525585	29.085606972346024	22.564902860619206	26.684564823509184
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.5
7	0.5
8	0.0
9	0.5
10	0.5
11	1.0
12	2.0
13	1.0
14	3.0
15	13.0
16	31.0
17	42.0
18	56.0
19	108.0
20	157.5
21	169.0
22	207.0
23	319.0
24	462.5
25	532.0
26	651.5
27	885.5
28	1151.5
29	1303.0
30	1538.5
31	1883.5
32	2268.5
33	2544.0
34	2790.5
35	3175.0
36	3635.0
37	3957.0
38	4137.5
39	4722.5
40	7191.0
41	9794.0
42	10333.0
43	9710.5
44	8430.5
45	7162.0
46	6551.0
47	6268.5
48	5645.0
49	5248.5
50	5193.0
51	5011.0
52	4709.0
53	4481.5
54	4374.0
55	4278.5
56	3994.5
57	3771.5
58	3737.0
59	3612.0
60	3324.5
61	3057.0
62	2952.0
63	2899.5
64	2701.0
65	2461.5
66	2321.0
67	2274.0
68	2162.0
69	2014.0
70	1928.0
71	1878.0
72	1831.5
73	1680.5
74	1498.0
75	1420.0
76	1336.0
77	1167.0
78	957.0
79	832.0
80	725.5
81	532.5
82	378.0
83	310.0
84	277.0
85	201.0
86	125.0
87	92.0
88	66.5
89	32.5
90	19.0
91	11.5
92	9.0
93	7.0
94	3.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	151226.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.93367542618333
#Duplication Level	Percentage of deduplicated	Percentage of total
1	59.89479259455426	32.90241096107812
2	24.907913426583526	27.365664634388263
3	10.000722247634638	16.48129289936916
4	3.465584900209452	7.615092642799519
5	1.1086501191708598	3.045111290386574
6	0.3490863567421817	1.150595797019031
7	0.10833714519584949	0.41659502995516645
8	0.051761080482461426	0.2274741115945671
9	0.02407492115463322	0.11902715141576183
>10	0.06740977923297302	0.6030709005065267
>50	0.006018730288658305	0.21689392035761046
>100	0.01083371451958495	1.428987078941452
>500	0.002407492115463322	1.0004893338447092
>1k	0.001203746057731661	0.8126909393887294
>5k	0.0	0.0
>10k+	0.001203746057731661	6.61460330895481
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT	10003	6.61460330895481	No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG	1229	0.8126909393887294	No Hit
ATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTCTT	774	0.5118167510877759	RNA PCR Primer, Index 24 (95% over 23bp)
CTCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC	739	0.4886725827569333	No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC	456	0.30153545025326334	No Hit
CTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTC	438	0.28963273511168713	No Hit
CTTTACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTC	291	0.1924272281221483	RNA PCR Primer, Index 24 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCACCTCGTATGCCGT	228	0.15076772512663167	No Hit
CTTAATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG	160	0.10580191236956608	No Hit
TATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTCT	156	0.10315686456032692	RNA PCR Primer, Index 24 (95% over 22bp)
CTTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG	154	0.10183434065570734	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	6.61261952309788E-4	0.0
3	0.0	0.0	0.0	6.61261952309788E-4	0.0
4	0.0	0.0	0.0	0.005951357570788092	0.0
5	0.0	0.0	0.0	0.007273881475407668	0.0
6	0.0	0.0	0.0	0.007273881475407668	0.0
7	0.0	0.0	0.0	0.009257667332337032	0.0
8	0.0	0.0	0.0	0.009257667332337032	0.0
9	0.0	0.0	0.0	0.01587028685543491	0.0
10	0.0	0.0	0.0	0.019176596616983854	0.0
11	0.0	0.0	0.0	0.021160382473913216	0.0
12	0.0	0.0	0.0	0.021821644426223002	0.0
13	0.0	0.0	0.0	0.02380543028315237	0.0
14	0.0	0.0	0.0	0.024466692235462155	0.0
15	0.0	0.0	0.0	0.02578921614008173	0.0
16	0.0	0.0	0.0	0.027111740044701307	0.0
17	0.0	0.0	0.0	0.027111740044701307	0.0
18	0.0	0.0	0.0	0.028434263949320884	0.0
19	0.0	0.0	0.0	0.02975678785394046	0.0
20	0.0	0.0	0.0	0.031079311758560036	0.0
21	0.0	0.0	0.0	0.03240183566317961	0.0
22	0.0	0.0	0.0	0.035708145424728555	0.0
23	0.0	0.0	0.0	0.04165950299551664	0.0
24	0.0	0.0	0.0	0.043643288852446005	0.0
25	0.0	0.0	0.0	0.05223969423247325	0.0
26	0.0	0.0	0.0	0.05686852789864177	0.0
27	0.0	0.0	0.0	0.11439831774959332	0.0
28	0.0	0.0	0.0	0.14613889146046316	0.0
29	0.0	0.0	0.0	0.19441101397907767	0.0
30	0.0	0.0	0.0	0.2327642072130454	0.0
31	0.0	0.0	0.0	0.2731011863039424	0.0
32	0.0	0.0	0.0	0.32732466639334507	0.0
33	0.0	0.0	0.0	0.3789030986735085	0.0
34	0.0	0.0	0.0	0.47809239151997673	0.0
35	0.0	0.0	0.0	0.6182799254096518	0.0
36	0.0	0.0	0.0	0.8649306336212027	0.0
37	0.0	0.0	0.0	1.2610265430547658	0.0
38	0.0	0.0	0.0	1.7305225291947153	0.0
39	0.0	0.0	0.0	2.1920833719069472	0.0
40	0.0	0.0	0.0	2.800444368031952	0.0
41	0.0	0.0	0.0	3.5443640643804635	0.0
42	0.0	0.0	0.0	4.451615462949492	0.0
43	0.0	0.0	0.0	5.39457500694325	0.0
44	0.0	0.0	0.0	6.2317326385674425	0.0
45	0.0	0.0	0.0	7.033182124766905	0.0
46	0.0	0.0	0.0	7.810164918730906	0.0
47	0.0	0.0	0.0	8.519698993559308	0.0
48	0.0	0.0	0.0	9.188896089296813	0.0
49	0.0	0.0	0.0	9.75956515414016	0.0
50	0.0	0.0	0.0	10.373877507835955	0.0
51	0.0	0.0	0.0	11.111845846613678	0.0
52	0.0	0.0	0.0	11.960244931427136	0.0
53	0.0	0.0	0.0	12.917752238371708	0.0
54	0.0	0.0	0.0	13.702670175763426	0.0
55	0.0	0.0	0.0	14.445267348207318	0.0
56	0.0	0.0	0.0	15.162736566463439	0.0
57	0.0	0.0	0.0	15.823998518773227	0.0
58	0.0	0.0	0.0	16.380119820665758	0.0
59	0.0	0.0	0.0	16.917725787893616	0.0
60	0.0	0.0	0.0	17.446735349741445	0.0
61	0.0	0.0	0.0	17.979712483303135	0.0
62	0.0	0.0	0.0	18.53186621348181	0.0
63	0.0	0.0	0.0	19.20833719069472	0.0
64	0.0	0.0	0.0	19.842487402959808	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGCGTCT	15	0.0022211592	70.0	23
CGAGATA	15	0.0022211592	70.0	27
GCATAAC	25	2.384379E-4	55.999996	11
CGATTCT	20	0.006940425	52.5	46
TCGAGAT	20	0.006940425	52.5	26
TCTCGTA	1605	0.0	48.84735	38
CATCTCG	1600	0.0	48.781246	36
ATCTCGT	1615	0.0	48.544895	37
TATGCCG	1675	0.0	48.268658	43
CTCGTAT	1670	0.0	48.203594	39
CGTATGC	1690	0.0	48.047337	41
TCGTATG	1695	0.0	47.90561	40
ATGCCGT	1680	0.0	47.708332	44
CCGTCTT	1670	0.0	47.574852	47
TGCTTGA	1685	0.0	47.566765	55
GTATGCC	1705	0.0	47.419357	42
TGCCGTC	1695	0.0	47.286137	45
CTTGAAA	1685	0.0	47.151337	57
CGTCTTC	1680	0.0	47.083332	48
GCCGTCT	1695	0.0	47.079647	46
>>END_MODULE