FastQCFastQC Report
Wed 25 May 2016
SRR1779732_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779732_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences97660
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGCCGT150.002219246769.99641452
TAGATCT600.064.1633839
GTAGATC650.059.22773738
CGACTAG252.3807831E-455.9971322
AGATCTC552.2519089E-950.90648340
CGCCGTA451.6704671E-646.6642853
GCCGTAT451.6704671E-646.6642854
CGTATCC305.8543455E-446.66427656
CACGTGT305.8543455E-446.66427633
TATCGTG305.8543455E-446.66427637
ATCTCGG703.765308E-1044.99769642
TGGTCGC551.2966848E-744.5431749
GGTGGTC1000.041.9978547
GACGCTT503.4493787E-641.9978514
GATCTCG1000.041.9978541
GTGGTCG1200.040.8312448
CCGTATC701.8982973E-839.9979555
GACGAAA350.001250449939.9979523
GTGTAGA1150.039.5631936
TGTAGAT1250.039.19799437