##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779732_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	97660
Sequences flagged as poor quality	0
Sequence length	76
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.054300634855622	34.0	31.0	34.0	21.0	34.0
2	28.325414704075364	34.0	23.0	34.0	12.0	34.0
3	27.37444194142945	33.0	23.0	34.0	11.0	34.0
4	29.88362686872824	34.0	31.0	34.0	21.0	34.0
5	29.347982797460578	34.0	27.0	34.0	11.0	34.0
6	31.95738275650215	37.0	28.0	38.0	12.0	38.0
7	33.00281589186975	37.0	32.0	38.0	21.0	38.0
8	32.18035019455253	37.0	31.0	38.0	11.0	38.0
9	33.661560516076186	37.0	34.0	38.0	21.0	38.0
10-11	33.391030104443985	38.0	34.0	38.0	21.0	38.0
12-13	32.317801556420235	37.5	30.0	38.0	16.0	38.0
14-15	32.45273909481876	37.0	32.0	38.0	11.5	38.0
16-17	32.09932418595126	37.5	29.5	38.0	16.0	38.0
18-19	32.59289371288143	37.0	32.0	38.0	16.0	38.0
20-21	31.565538603317634	36.5	28.5	38.0	16.0	38.0
22-23	31.52646938357567	36.5	28.5	38.0	16.0	38.0
24-25	32.855964570960474	37.0	32.5	38.0	16.5	38.0
26-27	31.30146938357567	37.0	27.5	38.0	11.0	38.0
28-29	33.51943989350809	37.5	34.0	38.0	21.0	38.0
30-31	33.94493139463445	38.0	35.0	38.0	21.0	38.0
32-33	34.034507474912964	38.0	35.0	38.0	21.0	38.0
34-35	34.101034200286705	38.0	36.0	38.0	21.0	38.0
36-37	31.89282715543723	37.0	29.0	38.0	16.0	38.0
38-39	30.025747491296336	35.0	24.0	38.0	11.0	38.0
40-41	31.428578742576285	36.5	28.5	38.0	11.0	38.0
42-43	32.509246364939585	37.0	30.5	38.0	16.0	38.0
44-45	34.125517100143355	38.0	35.5	38.0	21.5	38.0
46-47	32.626315789473686	37.5	31.0	38.0	16.0	38.0
48-49	31.98441019864837	36.5	29.5	38.0	16.0	38.0
50-51	29.789739913987304	35.0	23.5	38.0	11.0	38.0
52-53	33.271057751382344	37.0	33.5	38.0	21.0	38.0
54-55	32.305565226295315	37.0	30.0	38.0	16.0	38.0
56-57	32.7431855416752	37.5	31.0	38.0	16.0	38.0
58-59	33.85601576899447	38.0	35.0	38.0	21.0	38.0
60-61	34.317939791112025	38.0	36.0	38.0	22.0	38.0
62-63	34.210731107925454	38.0	36.0	38.0	21.5	38.0
64-65	33.905293876715135	38.0	35.0	38.0	16.5	38.0
66-67	31.89638541879992	37.0	28.0	38.0	11.0	38.0
68-69	31.91555908253123	37.0	29.0	38.0	11.0	38.0
70-71	31.42099119393815	37.0	27.0	38.0	11.0	38.0
72-73	30.238925865246774	35.5	24.5	38.0	11.0	38.0
74-75	32.389509522834324	37.0	30.5	38.0	16.0	38.0
76	32.9136289166496	37.0	33.0	38.0	19.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	6.0
12	23.0
13	129.0
14	439.0
15	742.0
16	1161.0
17	1688.0
18	1962.0
19	2085.0
20	1791.0
21	1418.0
22	1121.0
23	989.0
24	844.0
25	949.0
26	1001.0
27	1285.0
28	1646.0
29	2064.0
30	2729.0
31	3870.0
32	5282.0
33	7649.0
34	11266.0
35	15868.0
36	19086.0
37	10567.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.60557034609871	17.769813639156258	11.626049559696908	28.998566455048124
2	22.584476756092567	17.08171206225681	37.39913987302887	22.93467130862175
3	21.248208068810158	17.269096866680318	34.70612328486586	26.77657177964366
4	14.637517919311898	18.758959655949212	32.62031537988941	33.98320704484948
5	14.930370673766127	29.33647347941839	38.07188203972968	17.66127380708581
6	28.914601679295515	32.05304116321933	16.777595740323573	22.254761417161582
7	28.458939176735615	26.059799303706736	20.123899242269097	25.35736227728855
8	24.25967642842515	31.507270120827357	17.84456276878968	26.388490681957812
9	23.475322547614173	16.903542903952488	19.379479827974606	40.24165472045873
10-11	25.4500307188204	23.358079049764488	26.224656973172227	24.96723325824288
12-13	25.062973581814457	19.83207044849478	28.2454433749744	26.85951259471636
14-15	24.044132705304115	22.164140896989558	23.3852140077821	30.406512389924227
16-17	25.079868933032973	23.221380298996518	25.62000819168544	26.07874257628507
18-19	23.17376612738071	22.990989146016794	26.97214826950645	26.86309645709605
20-21	24.2806676223633	23.063690354290394	26.8943272578333	25.761314765513006
22-23	24.950849887364328	22.131886135572394	27.370468973991397	25.546795003071882
24-25	23.33350399344665	24.517202539422485	23.799406102805655	28.34988736432521
26-27	23.51986483719025	23.61714110178169	27.173356543108746	25.689637517919312
28-29	23.697522015154618	24.335961499078433	25.711652672537376	26.254863813229573
30-31	23.67909072291624	24.250972762645915	25.5954331353676	26.474503379070242
32-33	23.639156256399755	24.36156051607618	25.552426786811388	26.44685644071268
34-35	23.78967847634651	24.253020684005737	25.523243907433958	26.4340569322138
36-37	24.357464673356542	22.157485152570143	29.334937538398524	24.150112635674787
38-39	24.1045463854188	21.108949416342412	32.31978292033586	22.46672127790293
40-41	23.996518533688306	23.032459553553146	25.16127380708581	27.809748105672742
42-43	22.14468564407127	23.371902518943273	26.137620315379888	28.34579152160557
44-45	21.78681138644276	22.992525087036658	28.011468359614987	27.20919516690559
46-47	23.545975834527955	21.889719434773706	28.855211959860743	25.7090927708376
48-49	23.757423714929345	22.263465082940815	29.00419823878763	24.97491296334221
50-51	24.438357567069424	21.01013721073111	31.16117141101782	23.39033381118165
52-53	21.159123489657997	23.66373131271759	28.150727012082736	27.026418185541672
54-55	22.684824902723737	22.7329510546795	28.42822035633832	26.15400368625845
56-57	22.626971124308824	23.068298177349988	27.401187794388694	26.903542903952488
58-59	20.918492729879173	23.993958631988534	27.793364734794185	27.29418390333811
60-61	20.990169977472863	23.175814048740527	27.71451976244112	28.119496211345485
62-63	20.711652672537376	24.03696498054475	27.308007372516897	27.94337497440098
64-65	21.33217284456277	24.403030923612533	26.545156665984027	27.71963956584067
66-67	20.581097685848864	24.115297972557855	23.44613966823674	31.857464673356546
68-69	20.577421370121158	24.530166630821068	23.86190227465921	31.030509724398563
70-71	20.80534507474913	24.320602088879788	23.197317223018636	31.67673561335245
72-73	20.384497235306164	23.921257423714927	22.20817120622568	33.486074134753224
74-75	21.967028466106903	24.415830432111406	23.896170387057136	29.720970714724555
76	22.372516895351218	23.08724144992832	24.90579561744829	29.634446037272166
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.5
16	2.5
17	4.0
18	5.0
19	10.0
20	23.5
21	33.0
22	38.0
23	58.5
24	96.5
25	119.0
26	148.0
27	241.5
28	372.5
29	439.0
30	533.5
31	740.5
32	1006.5
33	1160.0
34	1355.5
35	1769.0
36	2205.5
37	2424.0
38	2779.0
39	3452.0
40	4100.5
41	4660.0
42	4889.0
43	5046.0
44	5398.0
45	5619.0
46	5645.0
47	5493.0
48	5250.0
49	4934.0
50	4709.0
51	4484.0
52	3925.0
53	3408.0
54	3225.0
55	2997.5
56	2578.0
57	2174.0
58	1962.0
59	1895.5
60	1777.5
61	1692.0
62	1658.0
63	1644.5
64	1618.5
65	1602.0
66	1612.0
67	1626.0
68	1574.5
69	1489.5
70	1397.0
71	1338.0
72	1287.5
73	1180.0
74	1038.5
75	954.0
76	846.0
77	646.5
78	489.5
79	424.0
80	350.5
81	237.0
82	156.5
83	116.0
84	92.5
85	57.5
86	31.0
87	16.0
88	14.0
89	9.0
90	6.0
91	4.0
92	2.0
93	2.5
94	2.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.019455252918287938
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	97660.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	95.22629531026008
#Duplication Level	Percentage of deduplicated	Percentage of total
1	96.49132239402998	91.88511161171411
2	2.6269382137250266	5.0030718820397295
3	0.5559259338910514	1.5881630145402417
4	0.188176089808383	0.7167724759369241
5	0.06989397621454226	0.3327872209707147
6	0.03225875825286566	0.18431292238378047
7	0.015054087184670637	0.10034814663116935
8	0.005376459708810942	0.040958427196395655
9	0.004301167767048753	0.0368625844767561
>10	0.010752919417621885	0.11161171411017817
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.005119803399549457	0.0
2	0.0	0.0	0.0	0.005119803399549457	0.0
3	0.0	0.0	0.0	0.005119803399549457	0.0
4	0.0	0.0	0.0	0.011263567479008805	0.0
5	0.0	0.0	0.0	0.011263567479008805	0.0
6	0.0	0.0	0.0	0.011263567479008805	0.0
7	0.0	0.0	0.0	0.011263567479008805	0.0
8	0.0	0.0	0.0	0.011263567479008805	0.0
9	0.0	0.0	0.0	0.015359410198648372	0.0
10	0.0	0.0	0.0	0.015359410198648372	0.0
11	0.0	0.0	0.0	0.016383370878558262	0.0
12	0.0	0.0	0.0	0.016383370878558262	0.0
13	0.0	0.0	0.0	0.016383370878558262	0.0
14	0.0	0.0	0.0	0.018431292238378045	0.0
15	0.0	0.0	0.0	0.018431292238378045	0.0
16	0.0	0.0	0.0	0.018431292238378045	0.0
17	0.0	0.0	0.0	0.018431292238378045	0.0
18	0.0	0.0	0.0	0.018431292238378045	0.0
19	0.0	0.0	0.0	0.02150317427810772	0.0
20	0.0	0.0	0.0	0.023551095637927504	0.0
21	0.0	0.0	0.0	0.02662297767765718	0.0
22	0.0	0.0	0.0	0.04300634855621544	0.0
23	0.0	0.0	0.0	0.04300634855621544	0.0
24	0.0	0.0	0.0	0.05529387671513414	0.0
25	0.0	0.0	0.0	0.05529387671513414	0.0
26	0.0	0.0	0.0	0.058365758754863814	0.0
27	0.0	0.0	0.0	0.0839647757526111	0.0
28	0.0	0.0	0.0	0.11570755682981773	0.0
29	0.0	0.0	0.0	0.14130657382756504	0.0
30	0.0	0.0	0.0	0.17202539422486177	0.0
31	0.0	0.0	0.0	0.1914806471431497	0.0
32	0.0	0.0	0.0	0.2181036248208069	0.0
33	0.0	0.0	0.0	0.24472660249846406	0.0
34	0.0	0.0	0.0	0.2856850296948597	0.0
35	0.0	0.0	0.0	0.35633831660864224	0.0
36	0.0	0.0	0.0	0.4515666598402621	0.0
37	0.0	0.0	0.0	0.6747900880606185	0.0
38	0.0	0.0	0.0	0.9174687691992628	0.0
39	0.0	0.0	0.0	1.2328486586115093	0.0
40	0.0	0.0	0.0	1.6076182674585295	0.0
41	0.0	0.0	0.0	2.085807904976449	0.0
42	0.0	0.0	0.0010239606799098914	2.584476756092566	0.0
43	0.0	0.0	0.0010239606799098914	3.124104034405079	0.0
44	0.0	0.0	0.0010239606799098914	3.6821626049559697	0.0
45	0.0	0.0	0.0010239606799098914	4.032357157485152	0.0
46	0.0	0.0	0.0010239606799098914	4.477780053245955	0.0
47	0.0	0.0	0.0010239606799098914	5.038910505836576	0.0
48	0.0	0.0	0.0010239606799098914	5.429039524882245	0.0
49	0.0	0.0	0.0010239606799098914	5.760802785173049	0.0
50	0.0	0.0	0.0010239606799098914	6.258447675609257	0.0
51	0.0	0.0	0.0010239606799098914	6.8840876510342	0.0
52	0.0	0.0	0.0010239606799098914	7.367397091951669	0.0
53	0.0	0.0	0.0010239606799098914	8.11488838828589	0.0
54	0.0	0.0	0.0010239606799098914	8.865451566659841	0.0
55	0.0	0.0	0.0010239606799098914	9.310874462420642	0.0
56	0.0	0.0	0.0010239606799098914	9.676428425148474	0.0
57	0.0	0.0	0.0010239606799098914	10.08601269711243	0.0
58	0.0	0.0	0.0010239606799098914	10.415728036043417	0.0
59	0.0	0.0	0.0010239606799098914	10.817120622568094	0.0
60	0.0	0.0	0.0010239606799098914	11.090518124104035	0.0
61	0.0	0.0	0.0010239606799098914	11.378251075158714	0.0
62	0.0	0.0	0.0010239606799098914	11.586115093180421	0.0
63	0.0	0.0	0.0010239606799098914	11.878967847634652	0.0
64	0.0	0.0	0.0010239606799098914	12.183084169567888	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGCCGT	15	0.0022192467	69.996414	52
TAGATCT	60	0.0	64.16338	39
GTAGATC	65	0.0	59.227737	38
CGACTAG	25	2.3807831E-4	55.99713	22
AGATCTC	55	2.2519089E-9	50.906483	40
CGCCGTA	45	1.6704671E-6	46.66428	53
GCCGTAT	45	1.6704671E-6	46.66428	54
CGTATCC	30	5.8543455E-4	46.664276	56
CACGTGT	30	5.8543455E-4	46.664276	33
TATCGTG	30	5.8543455E-4	46.664276	37
ATCTCGG	70	3.765308E-10	44.997696	42
TGGTCGC	55	1.2966848E-7	44.54317	49
GGTGGTC	100	0.0	41.99785	47
GACGCTT	50	3.4493787E-6	41.99785	14
GATCTCG	100	0.0	41.99785	41
GTGGTCG	120	0.0	40.83124	48
CCGTATC	70	1.8982973E-8	39.99795	55
GACGAAA	35	0.0012504499	39.99795	23
GTGTAGA	115	0.0	39.56319	36
TGTAGAT	125	0.0	39.197994	37
>>END_MODULE