FastQCFastQC Report
Wed 25 May 2016
SRR1779732_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779732_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences97660
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT64076.560516076182675No Hit
TCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG8600.8806061847225066No Hit
ATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTCTT8220.8416956788859308TruSeq Adapter, Index 12 (95% over 23bp)
CTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTC4480.45873438459963134TruSeq Adapter, Index 12 (95% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC4190.4290395248822445No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC3390.3471226704894532No Hit
CTTTACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTC3340.34200286708990374RNA PCR Primer, Index 12 (95% over 21bp)
CTTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG1530.1566659840262134No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC1460.14949825926684415No Hit
CTTAATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG1380.14130657382756504No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAACCTCGTATGCCGT1350.13823469178783535No Hit
TATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTCT1300.1331148883882859RNA PCR Primer, Index 12 (95% over 22bp)
TTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTC1260.12901904566864633TruSeq Adapter, Index 12 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGCTC150.00221879670.0000157
ATATCGT301.0144868E-770.0000137
GTACTAC150.00221879670.000011
CGAATGG150.00221879670.0000150
AATATCG352.947654E-760.036
ATCGTAT352.947654E-760.039
GAGTAAG252.3801807E-456.00000453
GATACGC200.006933097752.57
ACCATAA200.006933097752.513
GCGTTGA200.006933097752.540
GTAGCAG200.006933097752.511
CTCATTG200.006933097752.561
CCGAATC200.006933097752.540
TATCGTA407.4134005E-752.538
TTTACAC651.8189894E-1048.461542
CTGCTTG12600.045.55555354
TGAAAAA12350.045.34412859
CGTATGC12750.045.29411741
TATGCCG12850.045.2140143
GTATGCC12800.045.11718842
Produced by FastQC (version 0.11.5)