FastQCFastQC Report
Wed 25 May 2016
SRR1779731_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779731_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences134812
Sequences flagged as poor quality0
Sequence length76
%GC50

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTACG200.006939817752.4980516
GCGTAAC200.006939817752.4980536
GACTAAA200.006939817752.4980523
GCCGACT707.2759576E-1249.99814620
CGTATCA350.001252058339.9985256
AGACACG350.001252058339.9985269
CCGTGTA459.160744E-538.88744434
GCGTGTA459.160744E-538.88744434
GTGTCGA556.6570265E-638.180436
CTCGGTG654.8290894E-737.6909144
TGCCTAC951.8553692E-1036.8407419
CAGTTCT400.002412330134.99870370
TAGATCT708.626921E-734.99870339
CGTCGAT501.7028725E-434.99870337
ACGAGGC400.002412330134.99870324
TCGGTGG1055.4205884E-1033.33209645
GCTGCCT957.54153E-933.15666617
CTGCCTA957.54153E-933.15666618
TCTTCCG851.0535223E-732.93995311
ATATCTC751.4793641E-632.66545540
Produced by FastQC (version 0.11.5)