##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779731_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	134812
Sequences flagged as poor quality	0
Sequence length	76
%GC	50
>>END_MODULE
>>Per base sequence quality	warn
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.42875263329674	31.0	21.0	34.0	12.0	34.0
2	24.733421357149215	24.0	12.0	34.0	12.0	34.0
3	23.961398095125062	23.0	11.0	33.0	11.0	34.0
4	25.699025309319644	31.0	21.0	34.0	11.0	34.0
5	25.373075097172357	31.0	21.0	34.0	11.0	34.0
6	26.83603091712904	31.0	12.0	36.0	11.0	38.0
7	27.656284307034984	31.0	21.0	37.0	12.0	38.0
8	27.166609797347416	31.0	21.0	37.0	11.0	38.0
9	28.85864018039937	34.0	21.0	37.0	12.0	38.0
10-11	28.130199833842685	34.0	21.0	37.0	11.0	38.0
12-13	27.61453357267899	31.0	16.0	36.5	11.0	38.0
14-15	27.48423730825149	32.0	21.0	37.0	11.0	38.0
16-17	27.208379076046644	31.0	16.0	36.5	11.0	38.0
18-19	27.503015310209776	31.5	21.0	37.0	11.0	38.0
20-21	26.58130952734178	29.0	16.0	36.5	11.0	38.0
22-23	26.687449930273267	30.5	16.0	36.5	11.0	38.0
24-25	27.603870575319704	32.0	21.0	37.0	11.0	38.0
26-27	26.350068984956827	29.0	16.0	36.5	11.0	38.0
28-29	28.24095406937068	34.0	21.0	37.0	11.0	38.0
30-31	28.886382517876747	34.0	21.5	37.0	11.0	38.0
32-33	29.02822449040145	34.0	22.0	37.0	11.0	38.0
34-35	29.11978162181408	34.0	22.0	37.0	11.0	38.0
36-37	26.959580749488175	31.5	16.0	37.0	11.0	38.0
38-39	25.350558555618193	26.5	11.0	35.0	11.0	37.5
40-41	26.179231077352163	29.0	15.5	35.5	11.0	37.0
42-43	27.39950078628015	32.5	16.0	36.5	11.0	38.0
44-45	29.207051301071118	34.0	23.0	37.0	11.0	38.0
46-47	27.873468237248908	32.0	17.5	37.0	11.0	38.0
48-49	26.998631427469366	30.0	16.0	36.5	11.0	38.0
50-51	24.950219565023886	26.5	11.0	35.0	11.0	37.5
52-53	28.00183959884877	32.5	21.5	36.5	11.0	38.0
54-55	27.188707236744506	30.5	16.0	36.5	11.0	38.0
56-57	27.754179894964842	32.5	17.0	37.0	11.0	38.0
58-59	28.861889149333887	34.0	22.5	37.0	11.0	38.0
60-61	29.54237382428864	34.0	23.0	37.0	11.0	38.0
62-63	29.34487656885144	34.0	22.5	37.0	11.0	38.0
64-65	29.031573598789425	34.0	22.5	37.0	11.0	38.0
66-67	26.86721137584191	31.0	14.5	37.0	11.0	38.0
68-69	26.78357267899	31.0	16.0	37.0	11.0	38.0
70-71	26.3342172803608	29.0	15.0	36.5	11.0	38.0
72-73	25.19460433789277	26.5	11.0	35.0	11.0	37.5
74-75	27.06043230572946	30.0	16.0	36.5	11.0	37.5
76	27.02334361926238	31.0	21.0	36.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	4.0
12	30.0
13	155.0
14	393.0
15	711.0
16	1360.0
17	2799.0
18	4775.0
19	6597.0
20	7322.0
21	7241.0
22	6777.0
23	6551.0
24	6362.0
25	6296.0
26	6293.0
27	6387.0
28	6580.0
29	6667.0
30	7023.0
31	7406.0
32	7998.0
33	8185.0
34	8032.0
35	6939.0
36	4714.0
37	1215.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.96166513366763	17.062279322315522	10.469394415927367	33.50666112808949
2	19.004242945731832	17.1260718630389	42.10456042488799	21.765124766341277
3	17.84855947541762	15.129216983651306	42.030383051953834	24.991840488977243
4	14.433433225528885	19.508649081684123	32.79084947927484	33.267068213512154
5	13.019612497403793	27.188232501557724	42.65718185324749	17.134973147790998
6	25.239592914577337	35.62145803044239	17.823339168620002	21.315609886360264
7	27.26537696940925	24.72999436251966	20.272676022905973	27.73195264516512
8	22.43939708631279	34.157196688722074	18.081476426430882	25.321929798534253
9	23.121828917307063	16.17585971575231	19.410734949411033	41.2915764175296
10-11	24.22641985290572	23.142684414905258	27.76803165902019	24.862864073168833
12-13	23.04208824140284	18.501320357238228	31.874758923537964	26.581832477820967
14-15	24.811589472747233	22.52284663086372	22.98015013500282	29.685413761386226
16-17	27.884490566457607	21.37614372661086	24.610289181560923	26.12907652537061
18-19	21.841608468120302	21.58532469410992	28.534286763369593	28.038780074400183
20-21	27.206035071061923	20.959187609411625	25.7684775835979	26.06629973592855
22-23	28.06575082336884	20.38097498738985	25.572649319051717	25.980624870189594
24-25	22.71756223481589	25.127955968311426	23.69336557576477	28.46111622110791
26-27	25.58748479363855	23.725261846126457	25.53259353766727	25.154659822567726
28-29	23.548719698543156	24.08836008663917	25.817805536599113	26.545114678218557
30-31	22.961320065424687	24.048764385827617	26.14947537858417	26.840440170163525
32-33	23.511260125211404	23.9952674836068	25.863424620953623	26.63004777022817
34-35	23.624380618936	24.053125834495447	26.046642732101	26.275850814467557
36-37	26.803993709758778	20.8883482182595	29.547072960864018	22.760585111117702
38-39	25.849701806960805	19.3031777586565	34.56739756104798	20.27972287333472
40-41	23.699299765599502	25.186926979794084	24.027163753968487	27.086609500637927
42-43	21.463593745363916	25.46768833634988	25.492537756282825	27.576180162003382
44-45	21.959469483428776	22.98979319348426	27.620686585763877	27.430050737323086
46-47	26.34409399756698	20.944352134824793	27.183410972317006	25.52814289529122
48-49	25.92620065795574	21.39135014446097	28.19232780586226	24.490121391721033
50-51	30.716850132035724	18.83437676171261	28.456665578731865	21.992107527519806
52-53	21.60490164080349	22.803607987419518	28.157730765807198	27.433759605969794
54-55	25.155124006483128	21.503358393015432	27.08300107928478	26.258516521216663
56-57	24.477140303312403	21.79747276753096	26.815219769826758	26.91016715932988
58-59	21.35797020283878	23.681955916223764	27.38193700092351	27.578136880013947
60-61	21.128682906566183	22.81918523573569	27.530190190784204	28.521941666913925
62-63	20.868475124433466	23.569664196541826	27.451024026229316	28.110836652795395
64-65	20.936192623802036	24.709595584962763	26.50431712310477	27.849894668130432
66-67	18.974712745992537	27.93466408527494	22.629829910022178	30.460793258710346
68-69	19.368647525780844	27.244973662734623	23.45018176422583	29.936197047258702
70-71	19.351464823104852	27.898955207827225	22.33006828052503	30.419511688542894
72-73	18.26432365071359	28.882443699373944	20.880930480966086	31.97230216894639
74-75	20.968459781028397	24.564207934011808	24.474453312761476	29.992878972198323
76	22.187194018336648	22.631516482212266	25.005192416105395	30.1760970833457
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.5
11	0.5
12	0.0
13	0.5
14	1.0
15	2.5
16	4.5
17	5.0
18	9.0
19	17.5
20	31.5
21	41.0
22	48.5
23	75.0
24	112.5
25	131.0
26	171.0
27	264.0
28	401.0
29	485.0
30	625.0
31	887.0
32	1243.5
33	1478.0
34	1732.0
35	2282.0
36	2985.5
37	3393.0
38	3835.0
39	4681.5
40	5579.0
41	6435.5
42	6799.0
43	7046.0
44	7578.0
45	7831.5
46	7800.0
47	7746.0
48	7586.0
49	7149.5
50	6819.0
51	6390.0
52	5711.5
53	5060.0
54	4658.0
55	4331.0
56	3679.0
57	3101.5
58	2849.0
59	2637.0
60	2376.0
61	2274.5
62	2222.0
63	2182.5
64	2119.0
65	2078.0
66	2116.0
67	2171.0
68	2103.5
69	1956.0
70	1818.0
71	1760.0
72	1684.5
73	1510.5
74	1281.0
75	1150.0
76	1046.5
77	838.0
78	663.5
79	594.0
80	493.5
81	341.5
82	234.0
83	178.0
84	154.5
85	110.0
86	69.5
87	50.0
88	37.5
89	21.5
90	14.5
91	7.0
92	3.0
93	2.5
94	1.0
95	0.5
96	1.0
97	1.5
98	1.0
99	1.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	3.7088686467080085E-4
12-13	0.0
14-15	0.0
16-17	3.7088686467080085E-4
18-19	3.7088686467080085E-4
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	3.7088686467080085E-4
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	3.7088686467080085E-4
50-51	0.0
52-53	0.0
54-55	3.7088686467080085E-4
56-57	3.7088686467080085E-4
58-59	3.7088686467080085E-4
60-61	0.0
62-63	7.417737293416017E-4
64-65	0.0
66-67	7.417737293416017E-4
68-69	0.016319022045515236
70-71	3.7088686467080085E-4
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	134812.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	98.77011562433972
#Duplication Level	Percentage of deduplicated	Percentage of total
1	99.00924634090981	97.79154708970395
2	0.8044305908031525	1.5890740493076656
3	0.13920279246324962	0.41247227720478385
4	0.032115995234794505	0.12688402250925584
5	0.011005631434300545	0.054351374464236636
6	0.0029991745481197274	0.01777372901372174
7	0.0	0.0
8	9.994746065731762E-4	0.007897457796385926
9	0.0	0.0
>10	0.0	0.0
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.002225321188024805	0.0
10	0.0	0.0	0.0	0.002225321188024805	0.0
11	0.0	0.0	0.0	0.0029670949173664063	0.0
12	0.0	0.0	0.0	0.0029670949173664063	0.0
13	0.0	0.0	0.0	0.0029670949173664063	0.0
14	0.0	0.0	0.0	0.0029670949173664063	0.0
15	0.0	0.0	0.0	0.003708868646708008	0.0
16	0.0	0.0	0.0	0.003708868646708008	0.0
17	0.0	0.0	0.0	0.003708868646708008	0.0
18	0.0	0.0	0.0	0.003708868646708008	0.0
19	0.0	0.0	0.0	0.00445064237604961	0.0
20	0.0	0.0	0.0	0.00445064237604961	0.0
21	0.0	0.0	0.0	0.00445064237604961	0.0
22	0.0	0.0	0.0	0.005192416105391211	0.0
23	0.0	0.0	0.0	0.005934189834732813	0.0
24	0.0	0.0	0.0	0.00890128475209922	0.0
25	0.0	0.0	0.0	0.00964305848144082	0.0
26	0.0	0.0	0.0	0.00964305848144082	0.0
27	0.0	0.0	0.0	0.014093700857490432	0.0
28	0.0	0.0	0.0	0.014835474586832032	0.0
29	0.0	0.0	0.0	0.01780256950419844	0.0
30	0.0	0.0	0.0	0.02225321188024805	0.0
31	0.0	0.0	0.0	0.02670385425629766	0.0
32	0.0	0.0	0.0	0.03263804409103047	0.0
33	0.0	0.0	0.0	0.04079755511378809	0.0
34	0.0	0.0	0.0	0.051924161053912114	0.0
35	0.0	0.0	0.0	0.06601786191140255	0.0
36	0.0	0.0	0.0	0.07788624158086817	0.0
37	0.0	0.0	0.0	0.10829896448387384	0.0
38	0.0	0.0	0.0	0.15948135180844436	0.0
39	0.0	0.0	0.0	0.22179034507313888	0.0
40	0.0	0.0	0.0	0.3234133459929383	0.0
41	0.0	0.0	0.0	0.43022876301812896	0.0
42	0.0	0.0	0.0	0.5644898080289589	0.0
43	0.0	0.0	0.0	0.7551256564697505	0.0
44	0.0	0.0	0.0	0.904963949796754	0.0
45	0.0	0.0	0.0	1.025873067679435	0.0
46	0.0	0.0	0.0	1.1779366821944635	0.0
47	0.0	0.0	0.0	1.368572530635255	0.0
48	0.0	0.0	0.0	1.521377918879625	0.0
49	0.0	0.0	0.0	1.6882770079814853	0.0
50	0.0	0.0	0.0	1.929353470017506	0.0
51	0.0	0.0	0.0	2.2260629617541463	0.0
52	0.0	0.0	0.0	2.5524434026644514	0.0
53	0.0	0.0	0.0	2.9159125300418363	0.0
54	0.0	0.0	0.0	3.25119425570424	7.417737293416016E-4
55	0.0	0.0	0.0	3.5063644185977507	7.417737293416016E-4
56	0.0	0.0	0.0	3.7808206984541437	7.417737293416016E-4
57	0.0	0.0	0.0	4.034507313888971	7.417737293416016E-4
58	0.0	0.0	0.0	4.262973622526185	7.417737293416016E-4
59	0.0	0.0	0.0	4.540396997299943	7.417737293416016E-4
60	0.0	0.0	0.0	4.7302910720113935	7.417737293416016E-4
61	0.0	0.0	0.0	4.887547102631813	7.417737293416016E-4
62	0.0	0.0	0.0	5.018841052725277	7.417737293416016E-4
63	0.0	0.0	0.0	5.182031273180429	7.417737293416016E-4
64	0.0	0.0	0.0	5.357089873305047	7.417737293416016E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCTACG	20	0.0069398177	52.49805	16
GCGTAAC	20	0.0069398177	52.49805	36
GACTAAA	20	0.0069398177	52.49805	23
GCCGACT	70	7.2759576E-12	49.998146	20
CGTATCA	35	0.0012520583	39.99852	56
AGACACG	35	0.0012520583	39.99852	69
CCGTGTA	45	9.160744E-5	38.887444	34
GCGTGTA	45	9.160744E-5	38.887444	34
GTGTCGA	55	6.6570265E-6	38.1804	36
CTCGGTG	65	4.8290894E-7	37.69091	44
TGCCTAC	95	1.8553692E-10	36.84074	19
CAGTTCT	40	0.0024123301	34.998703	70
TAGATCT	70	8.626921E-7	34.998703	39
CGTCGAT	50	1.7028725E-4	34.998703	37
ACGAGGC	40	0.0024123301	34.998703	24
TCGGTGG	105	5.4205884E-10	33.332096	45
GCTGCCT	95	7.54153E-9	33.156666	17
CTGCCTA	95	7.54153E-9	33.156666	18
TCTTCCG	85	1.0535223E-7	32.939953	11
ATATCTC	75	1.4793641E-6	32.665455	40
>>END_MODULE