FastQCFastQC Report
Wed 25 May 2016
SRR1779731_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779731_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences134812
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT56364.180636738569267TruSeq Adapter, Index 5 (95% over 23bp)
TCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCG7480.554846749547518TruSeq Adapter, Index 5 (95% over 22bp)
ATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTCTT5970.4428389164169362TruSeq Adapter, Index 5 (96% over 26bp)
CTCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC4210.31228674005281426RNA PCR Primer, Index 5 (95% over 21bp)
CTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTC2830.20992196540367325TruSeq Adapter, Index 5 (95% over 24bp)
TCTTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC2650.19657003827552444RNA PCR Primer, Index 5 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTC2330.17283327893659317RNA PCR Primer, Index 5 (95% over 24bp)
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGACCTCGTATGCCGT1420.10533186956650743No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCAGTA150.00222063570.056
GAATTAG150.00222063570.063
CCCAATG252.3834471E-455.99999643
ACTAGGT252.3834471E-455.99999632
GTGCAAA200.00693879852.50000410
CGATTTC200.00693879852.50000442
ATCGTAT200.00693879852.50000440
AGTGCCT200.00693879852.50000458
TAACCGT200.00693879852.5000045
TCGTCTT200.00693879852.50000414
GTGACTC200.00693879852.50000448
GATAGTC200.00693879852.50000452
TCAGTAG200.00693879852.50000457
TCTCGTA9150.046.66666838
TCTGTCC305.8608525E-446.66666438
CCATAGG305.8608525E-446.6666641
ATCTCGT9150.045.90163837
AGTGATC9150.045.51912733
CTCGTAT9900.044.89898739
GTGATCT9300.044.78494634
Produced by FastQC (version 0.11.5)