FastQCFastQC Report
Wed 25 May 2016
SRR1779730_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779730_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62922
Sequences flagged as poor quality0
Sequence length76
%GC55

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTGT150.002215113370.033
TCCATAT207.8705874E-570.023
CCGATGA150.002215113370.021
TAGGTTG150.002215113370.048
TAGAACG150.002215113370.026
GTAGGTT207.8705874E-570.047
TGAGTAT207.8705874E-570.035
AGAACGG207.8705874E-570.027
TATTGTG150.002215113370.032
TCGTGTA301.0086114E-770.034
TATTCCA207.8705874E-570.020
GAAACTC150.002215113370.026
TTGGTGT207.8705874E-570.045
GATATCG207.8705874E-570.041
GTGTACT150.002215113370.036
TTCGGTG301.0086114E-770.044
TAGATAT150.002215113370.039
GCTAACA150.002215113370.063
TGTGTAC207.8705874E-570.035
CCTGAAA150.002215113370.062
Produced by FastQC (version 0.11.5)