##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779730_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	62922
Sequences flagged as poor quality	0
Sequence length	76
%GC	55
>>END_MODULE
>>Per base sequence quality	warn
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	23.50014303423286	23.0	12.0	34.0	12.0	34.0
2	23.95780490130638	23.0	12.0	34.0	12.0	34.0
3	23.575379040717078	23.0	11.0	33.0	11.0	34.0
4	23.583373065064684	23.0	12.0	34.0	11.0	34.0
5	23.777168557897078	23.0	11.0	34.0	11.0	34.0
6	24.927958424716316	26.0	12.0	36.0	11.0	38.0
7	25.959298814405138	27.0	12.0	37.0	12.0	38.0
8	25.103318394202347	27.0	11.0	36.0	11.0	38.0
9	26.56967356409523	28.0	12.0	37.0	12.0	38.0
10-11	25.62784876513779	27.0	11.0	37.0	11.0	38.0
12-13	25.921744381933188	28.0	11.0	36.5	11.0	38.0
14-15	24.87826197514383	26.5	11.0	36.0	11.0	38.0
16-17	24.977352913130545	26.5	11.0	35.5	11.0	38.0
18-19	25.32890721846095	27.0	11.5	36.0	11.0	38.0
20-21	24.486268713645465	25.5	11.0	35.0	11.0	38.0
22-23	24.652228155494104	25.5	11.0	35.5	11.0	38.0
24-25	24.783009122405517	26.0	11.0	36.0	11.0	38.0
26-27	23.993730332792982	24.0	11.0	34.5	10.0	38.0
28-29	24.811917930135724	26.0	11.0	36.5	11.0	38.0
30-31	25.242768824894313	26.5	11.0	37.0	11.0	38.0
32-33	25.401266647595435	27.0	11.0	37.0	11.0	38.0
34-35	25.480491719907185	27.0	11.0	37.0	11.0	38.0
36-37	24.646824640030516	25.5	11.0	35.0	11.0	38.0
38-39	24.33114808810909	25.5	11.0	34.0	11.0	37.5
40-41	23.918351292075904	25.0	11.0	34.0	11.0	37.5
42-43	24.52119290550205	26.0	11.0	35.0	11.0	38.0
44-45	25.491735799879216	27.0	11.0	37.0	11.0	38.0
46-47	24.89800069927847	26.0	11.0	35.5	11.0	38.0
48-49	24.119282603858746	25.0	11.0	35.0	11.0	38.0
50-51	23.301865802104196	23.0	11.0	34.0	11.0	37.5
52-53	24.428872254537364	25.0	11.0	35.5	11.0	38.0
54-55	24.119274657512477	24.5	11.0	35.0	11.0	38.0
56-57	24.466847843361624	24.5	11.0	36.0	11.0	38.0
58-59	24.86196401894409	26.0	11.0	37.0	11.0	38.0
60-61	25.44123676933346	27.0	11.0	37.0	11.0	38.0
62-63	25.4468389434538	27.0	11.0	37.0	11.0	38.0
64-65	25.333778328724453	26.5	11.0	37.0	11.0	38.0
66-67	24.45706589110327	25.0	11.0	35.5	11.0	38.0
68-69	24.27191602301262	25.0	11.0	35.5	11.0	38.0
70-71	24.08459680239026	25.0	11.0	34.5	11.0	38.0
72-73	23.694915927656464	24.5	11.0	34.0	11.0	37.5
74-75	23.866882807285208	24.5	11.0	35.0	11.0	38.0
76	23.62326372334001	24.0	11.0	34.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	fail
#Quality	Count
3	2.0
4	0.0
5	1.0
6	0.0
7	1.0
8	0.0
9	0.0
10	1.0
11	13.0
12	83.0
13	404.0
14	1086.0
15	1814.0
16	2974.0
17	4584.0
18	5845.0
19	6070.0
20	5141.0
21	3938.0
22	2647.0
23	1978.0
24	1492.0
25	1282.0
26	1127.0
27	1095.0
28	1149.0
29	1226.0
30	1411.0
31	1614.0
32	2061.0
33	2496.0
34	2956.0
35	3440.0
36	3445.0
37	1546.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.14243031054321	14.840596293824099	16.477543625441022	39.53942977019167
2	16.66030958965068	12.251676679062967	47.70509519722832	23.38291853405804
3	15.285591684943265	12.240551794284988	48.02453831728171	24.449318203490037
4	14.4687072883888	22.128985092654396	24.077429198054734	39.324878420902074
5	16.083404850449764	19.581386478497187	42.75452147102762	21.580687200025427
6	23.309812148374178	33.78627507072248	14.56406344362862	28.33984933727472
7	22.40746332702913	19.437071883790786	16.050285278364935	42.10517951081515
8	19.232664219075318	33.61782609386672	15.149636834660438	31.999872852397527
9	18.07749769541308	15.175307543151403	16.084427349883974	50.66276741155155
10-11	21.40563908579421	21.320607775199466	26.8508217044407	30.422931434565626
12-13	19.36693872036747	14.37460761485461	32.593993626473186	33.66446003830474
14-15	25.191118739967262	20.218852810756687	20.755256758689743	33.83477169058631
16-17	30.151704188752117	15.596366728386723	20.497945755223025	33.75398332763814
18-19	19.925935344416544	14.980609682443816	28.94243300804221	36.15102196509743
20-21	30.24364659323893	14.575883278500928	21.993356537770783	33.18711359048936
22-23	32.2763936901498	13.308697898041085	20.78436047204673	33.63054793976239
24-25	20.87684165355457	21.876539677998696	18.738377914461452	38.508240753985284
26-27	28.58630006357279	20.917832167832167	20.91942148760331	29.576446280991735
28-29	22.737964684753415	19.675296810184523	22.05851968403821	35.528218821023856
30-31	21.77454603250288	19.931656534350537	23.81849247029841	34.47530496284818
32-33	23.316671567186123	18.135365591995996	24.17095131002805	34.37701153078983
34-35	22.344162242919356	18.435106010998442	23.78731046759274	35.43342127848946
36-37	29.220572809053053	13.255348231030867	29.49394449918942	28.030134460726657
38-39	28.753526443358368	11.01442365001788	35.91846465609727	24.313585250526483
40-41	24.606031708189295	22.402352286724682	22.296658322406326	30.694957682679703
42-43	17.709277006945438	23.16708784309986	26.68033503393252	32.443300116022186
44-45	18.28902928438034	15.41224619541463	31.271108991933882	35.02761552827115
46-47	27.87437617216059	14.781938396007503	25.680250484757938	31.66343494707397
48-49	26.646587621984168	15.317556184239804	29.466130519088335	28.56972567468769
50-51	38.50328999650338	12.846880066117805	24.232334149210082	24.41749578816873
52-53	17.66374747683456	18.494206653209787	29.772557496384128	34.06948837357153
54-55	26.433612002924438	15.87780921199021	24.593915890524173	33.094662894561175
56-57	26.104612352585903	16.948250103309068	23.4002988016148	33.54683874249022
58-59	18.325585091588188	19.918146779512856	27.958834982318116	33.79743314658084
60-61	17.91075614892518	17.665196487463742	27.849962252155603	36.57408511145548
62-63	18.01088727301625	19.953113203798626	27.230897604005243	34.805101919179876
64-65	18.122506714982755	21.719989192453788	26.367234062842705	33.79027002972075
66-67	15.86747830509552	32.06634031596681	18.02186973521091	34.04431164372676
68-69	16.595920573599784	29.98839446113734	18.6054275766681	34.81025738859478
70-71	15.668261360204713	31.501819857908036	17.183750019867443	35.646168762019805
72-73	14.672949927286888	35.75180590764243	15.648020852372513	33.927223312698175
74-75	17.44067769672118	25.12198223112255	20.041640839809915	37.39569923234635
76	17.486251946978605	22.799516831431387	21.709208811468898	38.00502241012111
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.5
4	1.0
5	1.0
6	0.5
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	1.0
14	2.0
15	1.5
16	2.0
17	3.0
18	2.5
19	4.0
20	6.0
21	6.0
22	8.0
23	18.5
24	33.0
25	39.0
26	57.5
27	95.0
28	140.0
29	166.0
30	205.0
31	291.5
32	402.5
33	466.0
34	509.5
35	657.0
36	878.5
37	996.0
38	1071.0
39	1268.5
40	1475.5
41	1729.5
42	1899.0
43	1940.0
44	2123.5
45	2315.5
46	2365.0
47	2427.0
48	2442.0
49	2403.0
50	2411.0
51	2389.0
52	2329.0
53	2253.5
54	2216.0
55	2200.5
56	2097.5
57	2064.5
58	2119.0
59	2111.0
60	2123.0
61	2144.0
62	2145.0
63	2143.5
64	2133.0
65	2089.0
66	1959.0
67	1864.0
68	1878.0
69	1790.5
70	1632.0
71	1575.0
72	1450.0
73	1226.5
74	1063.0
75	998.0
76	923.0
77	735.5
78	558.0
79	493.0
80	422.5
81	285.0
82	178.5
83	139.0
84	112.0
85	60.0
86	28.5
87	22.0
88	16.0
89	6.0
90	3.0
91	3.0
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.5
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0015892692539970124
8	0.004767807761991036
9	0.0063570770159880495
10-11	0.0063570770159880495
12-13	0.008740980896983568
14-15	0.004767807761991036
16-17	0.005562442388989543
18-19	0.0063570770159880495
20-21	0.004767807761991036
22-23	0.0071517116429865545
24-25	0.004767807761991036
26-27	0.0031785385079940247
28-29	0.004767807761991036
30-31	0.0071517116429865545
32-33	0.005562442388989543
34-35	0.0063570770159880495
36-37	0.0063570770159880495
38-39	0.0071517116429865545
40-41	0.0071517116429865545
42-43	0.004767807761991036
44-45	0.0071517116429865545
46-47	0.0063570770159880495
48-49	0.0063570770159880495
50-51	0.0063570770159880495
52-53	0.00794634626998506
54-55	0.0063570770159880495
56-57	0.0063570770159880495
58-59	0.0071517116429865545
60-61	0.0071517116429865545
62-63	0.0071517116429865545
64-65	0.004767807761991036
66-67	0.0063570770159880495
68-69	0.033374654333937256
70-71	0.00794634626998506
72-73	0.005562442388989543
74-75	0.004767807761991036
76	0.0063570770159880495
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	62922.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	99.08458090969772
#Duplication Level	Percentage of deduplicated	Percentage of total
1	99.21887530876079	98.31060678300118
2	0.6720559458505758	1.3318076348494963
3	0.0866134154556828	0.257461619147516
4	0.016039521380682	0.06357077015988048
5	0.0032079042761364	0.01589269253997012
6	0.0016039521380682	0.009535615523982072
7	0.0016039521380682	0.011124884777979085
8	0.0	0.0
9	0.0	0.0
>10	0.0	0.0
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0031785385079940243	0.0
6	0.0	0.0	0.0	0.0031785385079940243	0.0
7	0.0	0.0	0.0	0.0031785385079940243	0.0
8	0.0	0.0	0.0	0.0031785385079940243	0.0
9	0.0	0.0	0.0	0.006357077015988049	0.0
10	0.0	0.0	0.0	0.006357077015988049	0.0
11	0.0	0.0	0.0	0.006357077015988049	0.0
12	0.0	0.0	0.0	0.00794634626998506	0.0
13	0.0	0.0	0.0	0.01589269253997012	0.0
14	0.0	0.0	0.0	0.019071231047964148	0.0
15	0.0	0.0	0.0	0.02066050030196116	0.0
16	0.0	0.0	0.0	0.02066050030196116	0.0
17	0.0	0.0	0.0	0.02066050030196116	0.0
18	0.0	0.0	0.0	0.02066050030196116	0.0
19	0.0	0.0	0.0	0.025428308063952194	0.0
20	0.0	0.0	0.0	0.028606846571946218	0.0
21	0.0	0.0	0.0	0.033374654333937256	0.0
22	0.0	0.0	0.0	0.04608880836591335	0.0
23	0.0	0.0	0.0	0.04926734687390738	0.0
24	0.0	0.0	0.0	0.0651600394138775	0.0
25	0.0	0.0	0.0	0.06674930866787451	0.0
26	0.0	0.0	0.0	0.06833857792187152	0.0
27	0.0	0.0	0.0	0.1160166555417819	0.0
28	0.0	0.0	0.0	0.17323034868567433	0.0
29	0.0	0.0	0.0	0.21455134928959665	0.0
30	0.0	0.0	0.0	0.255872349893519	0.0
31	0.0	0.0	0.0	0.29242554273545024	0.0
32	0.0	0.0	0.0	0.36235338991131877	0.0
33	0.0	0.0	0.0	0.4322812370871873	0.0
34	0.0	0.0	0.0	0.5498871618829662	0.0
35	0.0	0.0	0.0	0.7405994723626077	0.0
36	0.0	0.0	0.0	0.912240551794285	0.0
37	0.0	0.0	0.0	1.293665172753568	0.0
38	0.0	0.0	0.0	1.6099297542989732	0.0
39	0.0	0.0	0.0	2.0183719525762056	0.0
40	0.0	0.0	0.0	2.3950287657734974	0.0
41	0.0	0.0	0.0	2.7939353485267473	0.0
42	0.0	0.0	0.0	3.1006643145481707	0.0
43	0.0	0.0	0.0	3.4391786656495342	0.0
44	0.0	0.0	0.0	3.8110676710848352	0.0
45	0.0	0.0	0.0	4.057404405454372	0.0
46	0.0	0.0	0.0	4.341883601919838	0.0
47	0.0	0.0	0.0	4.672451606751216	0.0
48	0.0	0.0	0.0	4.8647531864848546	0.0
49	0.0	0.0	0.0	5.044340612186517	0.0
50	0.0	0.0	0.0	5.365373001493913	0.0
51	0.0	0.0	0.0	5.64349512094339	0.0
52	0.0	0.0	0.0	5.896188932328915	0.0
53	0.0	0.0	0.0	6.212453513874321	0.0
54	0.0	0.0	0.0	6.461968786751852	0.0
55	0.0	0.0	0.0	6.682877213057436	0.0
56	0.0	0.0	0.0	6.919678331902991	0.0
57	0.0	0.0	0.0	7.092908680588665	0.0
58	0.0	0.0	0.0	7.245478528972378	0.0
59	0.0	0.0	0.0	7.410762531388068	0.0
60	0.0	0.0	0.0	7.539493340961826	0.0
61	0.0	0.0	0.0	7.676170496805569	0.0
62	0.0	0.0	0.0	7.747687613235434	0.0
63	0.0	0.0	0.0	7.844633037729252	0.0
64	0.0	0.0	0.0	7.930453577445091	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTGT	15	0.0022151133	70.0	33
TCCATAT	20	7.8705874E-5	70.0	23
CCGATGA	15	0.0022151133	70.0	21
TAGGTTG	15	0.0022151133	70.0	48
TAGAACG	15	0.0022151133	70.0	26
GTAGGTT	20	7.8705874E-5	70.0	47
TGAGTAT	20	7.8705874E-5	70.0	35
AGAACGG	20	7.8705874E-5	70.0	27
TATTGTG	15	0.0022151133	70.0	32
TCGTGTA	30	1.0086114E-7	70.0	34
TATTCCA	20	7.8705874E-5	70.0	20
GAAACTC	15	0.0022151133	70.0	26
TTGGTGT	20	7.8705874E-5	70.0	45
GATATCG	20	7.8705874E-5	70.0	41
GTGTACT	15	0.0022151133	70.0	36
TTCGGTG	30	1.0086114E-7	70.0	44
TAGATAT	15	0.0022151133	70.0	39
GCTAACA	15	0.0022151133	70.0	63
TGTGTAC	20	7.8705874E-5	70.0	35
CCTGAAA	15	0.0022151133	70.0	62
>>END_MODULE