FastQCFastQC Report
Wed 25 May 2016
SRR1779730_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779730_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62922
Sequences flagged as poor quality0
Sequence length76
%GC51

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGT2524540.12110231715457No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG31224.961698610978672No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC18802.987826197514383No Hit
ATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTCTT10791.7148215250627763RNA PCR Primer, Index 48 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC8301.31909348081752No Hit
CTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTC7121.1315597088458726No Hit
CTTTACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTC4280.6802072407107211RNA PCR Primer, Index 48 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAACCTCGTATGCCGT3430.5451193541209751No Hit
CTTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG2800.44499539111916336No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC2690.42751342932519626No Hit
TTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTC2550.4052636597692381No Hit
CTTAATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG2130.33851435110136363No Hit
TATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTCT1800.28606846571946215RNA PCR Primer, Index 48 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCATCTCGTATGCCGTC1450.23044404182956676No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAACCTCGTATGCCG1320.2097835415276056No Hit
CCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG1000.1589269253997012No Hit
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAAACTCGTATGCCGT960.15256984838371315No Hit
TCTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGT940.14939130987571914No Hit
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAAGCTCGTATGCCGT930.14780204062172214No Hit
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATATCGTATGCCGT900.14303423285973108No Hit
CTTATTACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG690.10965957852579385No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAACCGA150.002215113370.068
CCGTGAA150.002215113370.036
CTTTACA550.070.01
TTACACA550.070.03
ACCAAAA150.002215113370.070
AACTCCC150.002215113370.070
AACCACA207.8705874E-570.070
CTGAAAA150.002215113370.058
AAAACCG150.002215113370.067
AATATCC207.8705874E-570.070
ATCGTAT150.002215113370.039
TTTACAC550.070.02
TCTTAAT401.3096724E-1070.01
TCTTTAT1150.066.956531
ACAAAAC252.373642E-456.00000470
AACCAAA252.373642E-456.00000469
CCTCTGC252.373642E-456.00000451
CATGATG200.006921677452.525
ACTCGTA200.006921677452.539
AAACTCT200.006921677452.568
Produced by FastQC (version 0.11.5)