##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779730_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	62922
Sequences flagged as poor quality	0
Sequence length	76
%GC	51
>>END_MODULE
>>Per base sequence quality	warn
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.67807761991037	34.0	34.0	34.0	34.0	34.0
2	33.66847843361622	34.0	34.0	34.0	34.0	34.0
3	33.68389434538	34.0	34.0	34.0	34.0	34.0
4	33.71494866660309	34.0	34.0	34.0	34.0	34.0
5	33.73891484695337	34.0	34.0	34.0	34.0	34.0
6	37.43056482629287	38.0	38.0	38.0	37.0	38.0
7	37.42595594545628	38.0	38.0	38.0	37.0	38.0
8	37.32788213979212	38.0	38.0	38.0	36.0	38.0
9	37.354550077874194	38.0	38.0	38.0	37.0	38.0
10-11	37.235426400940845	38.0	38.0	38.0	36.0	38.0
12-13	37.2342741807317	38.0	38.0	38.0	36.0	38.0
14-15	37.24880010171323	38.0	38.0	38.0	36.0	38.0
16-17	37.09159753345412	38.0	38.0	38.0	35.5	38.0
18-19	37.067353230984395	38.0	38.0	38.0	35.0	38.0
20-21	37.16426687009313	38.0	38.0	38.0	36.0	38.0
22-23	37.14412288229872	38.0	38.0	38.0	36.0	38.0
24-25	37.052199548647536	38.0	38.0	38.0	35.0	38.0
26-27	36.867852261530146	38.0	38.0	38.0	34.5	38.0
28-29	36.88901338164712	38.0	37.5	38.0	34.5	38.0
30-31	36.87950955150822	38.0	37.5	38.0	35.0	38.0
32-33	36.617717173643555	38.0	37.0	38.0	34.0	38.0
34-35	36.295246495661296	38.0	37.0	38.0	33.5	38.0
36-37	36.111574647976866	38.0	37.0	38.0	32.0	38.0
38-39	34.7383506563682	37.0	34.5	38.0	27.0	38.0
40-41	36.16699246686373	38.0	36.5	38.0	33.0	38.0
42-43	36.811647754362546	38.0	37.0	38.0	34.5	38.0
44-45	36.81834652426814	38.0	37.0	38.0	34.5	38.0
46-47	36.836027144718855	38.0	37.0	38.0	35.0	38.0
48-49	36.776826865007465	38.0	37.0	38.0	34.5	38.0
50-51	36.598558532786626	38.0	37.0	38.0	34.0	38.0
52-53	36.61096277931407	38.0	37.0	38.0	34.0	38.0
54-55	36.608491465624105	38.0	37.0	38.0	34.0	38.0
56-57	36.583500206605	38.0	37.0	38.0	34.0	38.0
58-59	36.39440895076444	38.0	37.0	38.0	34.0	38.0
60-61	36.37757858936461	38.0	37.0	38.0	34.0	38.0
62-63	36.511363275166076	38.0	37.0	38.0	34.0	38.0
64-65	36.57355932742125	38.0	37.0	38.0	34.0	38.0
66-67	36.36717682209719	38.0	37.0	38.0	34.0	38.0
68-69	35.84080289882712	38.0	37.0	38.0	31.0	38.0
70-71	32.11069260354089	36.0	29.0	38.0	18.5	38.0
72-73	25.85527319538476	24.0	15.0	38.0	10.0	38.0
74-75	25.043236070054988	24.5	11.0	38.0	11.0	38.0
76	24.94248434569785	25.0	11.0	37.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	0.0
19	0.0
20	2.0
21	1.0
22	4.0
23	12.0
24	25.0
25	67.0
26	108.0
27	180.0
28	345.0
29	596.0
30	949.0
31	1437.0
32	2161.0
33	3438.0
34	6434.0
35	16369.0
36	14734.0
37	16059.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.93738279139252	6.89901783160103	11.623915323734145	61.5396840532723
2	11.350561012046661	6.825911445917167	61.244079972028864	20.57944757000731
3	11.603254823432184	8.844283398493372	60.59247957789009	18.959982200184356
4	9.325831982454467	48.499729824226826	21.706239471091195	20.46819872222752
5	10.514605384444232	20.65891103270716	57.35990591526017	11.466577667588442
6	17.911064492546327	56.876768062045066	11.36168589682464	13.850481548583963
7	15.651123613362575	18.48955850100124	10.451034614284351	55.408283271351834
8	13.952194780839768	58.27850354407044	7.545850417977813	20.22345125711198
9	11.700200247926004	13.774196624392104	5.614888274371444	68.91071485331045
10-11	15.162423317758494	33.8848097644703	30.044340612186517	20.908426305584694
12-13	14.841390928451098	7.951908712374051	35.23648326499475	41.97021709418009
14-15	15.539080130955787	6.475477575410826	11.783636883760847	66.20180540987253
16-17	40.48822351482788	28.95410190394457	5.686405390801309	24.871269190426244
18-19	42.021868344935	12.826197514382887	5.548933600330568	39.603000540351545
20-21	17.43110517783923	7.427449858555037	5.836591335304027	69.30485362830171
22-23	16.60150662725279	32.27011220240934	5.591843870188487	45.53653730014939
24-25	40.69800705635549	32.40520008899908	5.488541368678682	21.408251485966755
26-27	41.51727535679095	35.04100314675313	8.035345348208892	15.406376148247036
28-29	16.78586186071644	12.26598010234894	31.20212326372334	39.74603477321128
30-31	15.020183719525763	5.8604303741139825	60.52255173071421	18.596834175646038
32-33	36.29732049203776	7.098471122977655	16.7024252248816	39.90178316010298
34-35	19.787991481516798	8.517688566796986	32.27011220240934	39.42420774927689
36-37	13.932328915164808	53.15708337306506	11.495979148787388	21.41460856298274
38-39	14.219986650138267	14.58472394393058	33.013890213279936	38.18139919265121
40-41	14.370967229267983	6.6955913670894125	36.00648421855631	42.926957185086295
42-43	38.18378309653222	6.988016909824863	34.58329360160198	20.24490639204094
44-45	15.932424271320048	30.819109373510063	37.4495407011856	15.798925653984297
46-47	37.81189409109692	8.889577572232287	15.48901814945488	37.80951018721591
48-49	37.32716696862782	6.4023711897269635	10.529703442357203	45.740758399288005
50-51	17.012332729411018	6.928419312799974	35.73710308000381	40.32214487778519
52-53	11.915546231842598	6.844188042338133	59.82406789358253	21.41619783223674
54-55	12.563968087473379	6.567655192142653	40.74568513397539	40.12269158640857
56-57	36.56034455357427	6.61136009662757	16.818441880423382	40.009853469374775
58-59	15.329296589428182	8.957916150154158	55.219160230126185	20.49362703029147
60-61	36.04383204602524	33.20857569689456	15.774291980547344	14.97330027653285
62-63	13.709831219605226	64.2056832268523	7.265344394647341	14.81914115889514
64-65	11.12250087409809	67.45812275515718	6.6304313276755344	14.788945043069196
66-67	11.391087378023585	67.64645116175583	6.5390483455707065	14.423413114649883
68-69	11.096277931407139	67.15059915450877	7.076221353421697	14.676901560662408
70-71	11.476907917739423	61.90918915482661	9.34490321350243	17.268999713931535
72-73	13.006579574711546	48.242268205079306	14.08489876354852	24.666253456660627
74-75	14.112710975493467	41.90505705476622	17.092590826737865	26.889641143002446
76	13.772607355138108	42.93728743523727	16.752487206382504	26.53761800324211
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	1.0
17	2.0
18	1.5
19	0.5
20	0.0
21	0.0
22	0.0
23	2.5
24	8.5
25	12.0
26	14.0
27	16.0
28	14.0
29	12.0
30	15.5
31	18.0
32	23.5
33	30.0
34	27.5
35	27.5
36	46.5
37	63.0
38	231.5
39	5190.5
40	10901.5
41	10165.5
42	8509.0
43	6627.5
44	3427.5
45	1432.5
46	756.0
47	537.0
48	259.0
49	163.5
50	127.0
51	165.5
52	222.0
53	248.0
54	256.0
55	360.5
56	556.5
57	730.0
58	812.0
59	917.0
60	1045.5
61	1137.5
62	1206.0
63	1252.5
64	1343.0
65	1377.0
66	1383.5
67	1400.0
68	1439.5
69	1479.5
70	1466.5
71	1453.0
72	1438.5
73	1344.0
74	1243.5
75	1223.0
76	1104.5
77	882.5
78	744.0
79	709.0
80	644.5
81	479.0
82	316.0
83	254.0
84	197.5
85	112.0
86	69.5
87	56.0
88	41.5
89	18.0
90	7.0
91	3.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	62922.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.525952766917772
#Duplication Level	Percentage of deduplicated	Percentage of total
1	55.02938593528339	12.946187343059663
2	25.035465783962714	11.779663710625854
3	11.45713706681078	8.086201964336798
4	4.931432817672094	4.6406662216712755
5	1.8847530905897452	2.217030609325832
6	0.695804904411268	0.9821683989701535
7	0.2634601094372762	0.43387050634118435
8	0.12159697358643518	0.22885477257556974
9	0.054043099371748976	0.11442738628778487
>10	0.3783016956022428	1.8133562188105907
>50	0.04728771195028035	0.9535615523982074
>100	0.06079848679321759	3.5679094752232925
>500	0.013510774842937244	2.450653189663393
>1k	0.020266162264405863	9.664346333555832
>5k	0.0	0.0
>10k+	0.006755387421468622	40.12110231715457
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGT	25245	40.12110231715457	No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG	3122	4.961698610978672	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC	1880	2.987826197514383	No Hit
ATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTCTT	1079	1.7148215250627763	RNA PCR Primer, Index 48 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC	830	1.31909348081752	No Hit
CTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTC	712	1.1315597088458726	No Hit
CTTTACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTC	428	0.6802072407107211	RNA PCR Primer, Index 48 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAACCTCGTATGCCGT	343	0.5451193541209751	No Hit
CTTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG	280	0.44499539111916336	No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC	269	0.42751342932519626	No Hit
TTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTC	255	0.4052636597692381	No Hit
CTTAATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG	213	0.33851435110136363	No Hit
TATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTCT	180	0.28606846571946215	RNA PCR Primer, Index 48 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCATCTCGTATGCCGTC	145	0.23044404182956676	No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAACCTCGTATGCCG	132	0.2097835415276056	No Hit
CCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG	100	0.1589269253997012	No Hit
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAAACTCGTATGCCGT	96	0.15256984838371315	No Hit
TCTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGT	94	0.14939130987571914	No Hit
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAAGCTCGTATGCCGT	93	0.14780204062172214	No Hit
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATATCGTATGCCGT	90	0.14303423285973108	No Hit
CTTATTACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG	69	0.10965957852579385	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0031785385079940243	0.0
2	0.0	0.0	0.0	0.0031785385079940243	0.0
3	0.0	0.0	0.0	0.0031785385079940243	0.0
4	0.0	0.0	0.0	0.004767807761991037	0.0
5	0.0	0.0	0.0	0.00794634626998506	0.0
6	0.0	0.0	0.0	0.00794634626998506	0.0
7	0.0	0.0	0.0	0.00794634626998506	0.0
8	0.0	0.0	0.0	0.00794634626998506	0.0
9	0.0	0.0	0.0	0.011124884777979085	0.0
10	0.0	0.0	0.0	0.011124884777979085	0.0
11	0.0	0.0	0.0	0.011124884777979085	0.0
12	0.0	0.0	0.0	0.01589269253997012	0.0
13	0.0	0.0	0.0	0.034963923587934265	0.0
14	0.0	0.0	0.0	0.04291026985791933	0.0
15	0.0	0.0	0.0	0.04449953911191634	0.0
16	0.0	0.0	0.0	0.047678077619910365	0.0
17	0.0	0.0	0.0	0.047678077619910365	0.0
18	0.0	0.0	0.0	0.052445885381901404	0.0
19	0.0	0.0	0.0	0.057213693143892436	0.0
20	0.0	0.0	0.0	0.0651600394138775	0.0
21	0.0	0.0	0.0	0.06992784717586853	0.0
22	0.0	0.0	0.0	0.07469565493785957	0.0
23	0.0	0.0	0.0	0.07628492419185659	0.0
24	0.0	0.0	0.0	0.0794634626998506	0.0
25	0.0	0.0	0.0	0.08899907822383268	0.0
26	0.0	0.0	0.0	0.09535615523982073	0.0
27	0.0	0.0	0.0	0.16687327166968627	0.0
28	0.0	0.0	0.0	0.23362258033756078	0.0
29	0.0	0.0	0.0	0.33374654333937254	0.0
30	0.0	0.0	0.0	0.39572804424525604	0.0
31	0.0	0.0	0.0	0.4958520072470678	0.0
32	0.0	0.0	0.0	0.6071008550268586	0.0
33	0.0	0.0	0.0	0.7628492419185658	0.0
34	0.0	0.0	0.0	1.0155430533040908	0.0
35	0.0	0.0	0.0	1.3238612885795111	0.0
36	0.0	0.0	0.0	1.830838180604558	0.0
37	0.0	0.0	0.0	2.495152728775309	0.0
38	0.0	0.0	0.0	3.0418613521502813	0.0
39	0.0	0.0	0.0	3.677569053749086	0.0
40	0.0	0.0	0.0	4.267187946981978	0.0
41	0.0	0.0	0.0	4.848860493944884	0.0
42	0.0	0.0	0.0	5.2970344235720415	0.0
43	0.0	0.0	0.0	5.783350815295127	0.0
44	0.0	0.0	0.0	6.333237977178094	0.0
45	0.0	0.0	0.0	6.873589523537078	0.0
46	0.0	0.0	0.0	7.391691300340104	0.0
47	0.0	0.0	0.0	7.881186230571183	0.0
48	0.0	0.0	0.0	8.243539620482503	0.0
49	0.0	0.0	0.0	8.60112520263183	0.0
50	0.0	0.0	0.0	8.99526397762309	0.0
51	0.0	0.0	0.0	9.329010520962461	0.0
52	0.0	0.0	0.0	9.705667334159754	0.0
53	0.0	0.0	0.0	10.050538762277105	0.0
54	0.0	0.0	0.0	10.347732112774546	0.0
55	0.0	0.0	0.0	10.703728425669876	0.0
56	0.0	0.0	0.0	11.029528622739264	0.0
57	0.0	0.0	0.0	11.306061472934745	0.0
58	0.0	0.0	0.0	11.576237246114237	0.0
59	0.0	0.0	0.0	11.822573980483774	0.0
60	0.0	0.0	0.0	12.0752677918693	0.0
61	0.0	0.0	0.0	12.401067988938687	0.0
62	0.0	0.0	0.0	12.615619338228283	0.0
63	0.0	0.0	0.0	12.841295572295857	0.0
64	0.0	0.0	0.0	13.070150344871427	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAACCGA	15	0.0022151133	70.0	68
CCGTGAA	15	0.0022151133	70.0	36
CTTTACA	55	0.0	70.0	1
TTACACA	55	0.0	70.0	3
ACCAAAA	15	0.0022151133	70.0	70
AACTCCC	15	0.0022151133	70.0	70
AACCACA	20	7.8705874E-5	70.0	70
CTGAAAA	15	0.0022151133	70.0	58
AAAACCG	15	0.0022151133	70.0	67
AATATCC	20	7.8705874E-5	70.0	70
ATCGTAT	15	0.0022151133	70.0	39
TTTACAC	55	0.0	70.0	2
TCTTAAT	40	1.3096724E-10	70.0	1
TCTTTAT	115	0.0	66.95653	1
ACAAAAC	25	2.373642E-4	56.000004	70
AACCAAA	25	2.373642E-4	56.000004	69
CCTCTGC	25	2.373642E-4	56.000004	51
CATGATG	20	0.0069216774	52.5	25
ACTCGTA	20	0.0069216774	52.5	39
AAACTCT	20	0.0069216774	52.5	68
>>END_MODULE