FastQCFastQC Report
Wed 25 May 2016
SRR1779729_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779729_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences138658
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTGG150.002221404669.9949545
GCCGTAT700.059.99567854
TCGGTGG1300.059.22649845
CTGTCGT252.3845349E-455.9959652
TCTCGGT1250.053.1961643
TACGGCC200.006921407852.53410367
ACGTTAC200.00694119452.49621641
CGACTTT200.00694119452.49621625
TGGGACC200.00694119452.4962164
GTAACAC200.00694119452.49621618
CTCGGTG1450.050.68644
ATCTCGG1050.049.9963942
GTGTACA305.8635196E-446.663336
CTCTCGG604.8894435E-946.663342
TAGATCT851.8189894E-1245.29084839
CGGTGGT1400.044.99675846
CTGTGTA551.3021963E-744.54224434
CGCCGTA802.910383E-1143.74684553
TGCCGTA404.575777E-543.74684553
CCGTATC1000.041.9969755