##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779729_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	138658
Sequences flagged as poor quality	0
Sequence length	76
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.995528566689263	34.0	23.0	34.0	12.0	34.0
2	27.540769375009013	33.0	23.0	34.0	12.0	34.0
3	26.53765379566992	32.0	21.0	34.0	11.0	34.0
4	28.776861053815864	33.0	23.0	34.0	12.0	34.0
5	28.37748272728584	34.0	23.0	34.0	11.0	34.0
6	30.639047151985462	36.0	26.0	38.0	11.0	38.0
7	31.584755297206076	37.0	27.0	38.0	12.0	38.0
8	30.829854750537294	36.0	26.0	38.0	11.0	38.0
9	32.28222677378874	37.0	31.0	38.0	12.0	38.0
10-11	31.980405025314084	37.0	31.0	38.0	11.0	38.0
12-13	31.026421122474	35.5	27.0	38.0	11.0	38.0
14-15	31.029392462028877	36.5	26.5	38.0	11.0	38.0
16-17	30.68471346766865	35.5	26.5	38.0	11.0	38.0
18-19	31.239243318091994	36.0	26.5	38.0	11.5	38.0
20-21	30.11619596417084	35.0	24.0	38.0	11.0	38.0
22-23	30.16378788097333	35.5	26.0	38.0	11.0	38.0
24-25	31.323017784765394	36.5	28.5	38.0	11.0	38.0
26-27	29.905786178943877	35.0	24.0	38.0	11.0	38.0
28-29	31.98349896868554	37.0	30.0	38.0	11.0	38.0
30-31	32.46635967632592	37.0	31.0	38.0	11.0	38.0
32-33	32.562084409121724	37.0	31.0	38.0	11.0	38.0
34-35	32.6132498665782	37.0	32.0	38.0	11.0	38.0
36-37	30.52855226528581	36.0	25.0	38.0	11.0	38.0
38-39	28.767398923971207	34.0	22.0	37.5	11.0	38.0
40-41	29.91674119055518	35.0	24.5	37.5	11.0	38.0
42-43	30.960103275685498	36.0	27.5	38.0	11.0	38.0
44-45	32.66498507118233	37.0	32.0	38.0	11.0	38.0
46-47	31.216092832725124	36.0	28.5	38.0	11.0	38.0
48-49	30.65585469284138	35.5	25.0	38.0	11.0	38.0
50-51	28.448026799751908	34.0	21.0	37.5	11.0	38.0
52-53	31.791656449681952	36.5	29.0	38.0	11.0	38.0
54-55	30.818301143821486	36.0	27.0	38.0	11.0	38.0
56-57	31.288447114483116	36.5	27.5	38.0	11.0	38.0
58-59	32.398090986455884	37.0	31.5	38.0	11.0	38.0
60-61	32.903362950569026	37.5	34.0	38.0	11.0	38.0
62-63	32.76198993206306	37.5	33.0	38.0	11.0	38.0
64-65	32.47150182463327	37.5	32.0	38.0	11.0	38.0
66-67	30.371871799679788	36.0	24.5	38.0	11.0	38.0
68-69	30.460326847351034	36.0	25.0	38.0	11.0	38.0
70-71	30.085498132094795	35.0	24.0	38.0	11.0	38.0
72-73	28.92698582122921	34.0	22.5	37.5	11.0	38.0
74-75	30.984548313115724	35.5	26.5	38.0	11.0	38.0
76	31.37246318279508	36.0	27.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	1.0
4	1.0
5	0.0
6	1.0
7	0.0
8	0.0
9	1.0
10	0.0
11	6.0
12	37.0
13	237.0
14	607.0
15	1067.0
16	1724.0
17	2835.0
18	3668.0
19	4099.0
20	3555.0
21	2966.0
22	2505.0
23	2307.0
24	2373.0
25	2524.0
26	2720.0
27	3127.0
28	3824.0
29	4546.0
30	5424.0
31	6821.0
32	8381.0
33	10947.0
34	14344.0
35	18035.0
36	19697.0
37	10278.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.43545990855198	19.499776428334464	10.737930736055619	29.32683292705794
2	21.163582339280822	17.16958271430426	37.914869679354965	23.75196526705996
3	20.66234908912576	17.548933346795714	36.425593907311516	25.363123656767012
4	14.521445004579647	19.14652704154857	33.2201042861161	33.11192366775568
5	14.692264420372428	28.497454167808566	39.15893781822902	17.65134359358998
6	27.1791025400626	32.99917783322996	17.37079721328737	22.45092241342007
7	27.474072898786943	25.766273853654315	21.1433887694904	25.61626447806834
8	23.642854567090982	31.181709999639395	18.365006671234358	26.810428762035265
9	23.411175859681514	16.073592199399954	20.006346642049387	40.508885298869146
10-11	24.476210395736118	23.420697712995754	27.24895963304076	24.854132258227374
12-13	24.14544169458398	19.68107548673492	29.479962640683432	26.69352017799767
14-15	23.885543254841153	22.32627745122787	23.866070462659117	29.92210883127186
16-17	25.13027266203395	22.97883542550928	26.037583437611566	25.853308474845203
18-19	21.972680196748744	23.129516638539098	27.94329770507883	26.95450545963333
20-21	24.552666690707152	22.632072409938335	27.2849879196567	25.53027297969781
22-23	25.18562325780988	21.652019948865707	27.70952259032343	25.452834203000986
24-25	22.400761597045925	24.952400184629585	24.425196168936072	28.22164204938841
26-27	23.5659602395866	23.85408440343153	27.27443195545795	25.30552340152392
28-29	23.049933107594	24.66328418274068	26.63796243207085	25.64882027759447
30-31	22.63888989059018	24.633798042595544	26.7026317497638	26.024680317050482
32-33	22.779560780354117	24.56348490858606	26.512567163102663	26.14438714795716
34-35	23.010627057707772	24.41410546281972	26.613441226637242	25.961826252835262
36-37	24.499105674638663	21.940902403138793	30.164007731586995	23.39598419063555
38-39	24.243167319973892	20.788151759602176	33.248709913561505	21.71997100686243
40-41	23.34721696327149	23.279782189286166	26.04568976397829	27.327311083464057
42-43	21.08787606694289	23.86418137037478	27.38081071432435	27.66713184835798
44-45	20.924747933705483	22.73428822825162	29.356888369610683	26.984075468432213
46-47	23.350402077097833	21.503011070642962	29.586383469763085	25.560203382496123
48-49	22.934509406542208	22.1332314005777	30.363459991994436	24.56879920088566
50-51	25.392251964415145	20.759153146850625	31.36789646206939	22.480698426664837
52-53	19.913020273632736	23.74633076817667	29.334381513562636	27.00626744462796
54-55	22.173779773173944	22.42224265700222	28.888047456771424	26.515930113052416
56-57	21.824672748909162	22.982943276477588	28.47174642097292	26.72063755364033
58-59	19.86260615567696	24.04428337029624	28.778420872324695	27.314689601702096
60-61	19.7553613864778	23.214704280815127	28.63252640575247	28.397407926954603
62-63	19.6728523724694	24.015347666476742	28.53129755576872	27.780502405285134
64-65	20.190040027406152	24.319353791785367	27.30373949731348	28.186866683495005
66-67	19.10500939419183	25.323928322857274	23.774698069592752	31.796364213358142
68-69	19.381273737858297	25.460690287000396	23.9897997092845	31.168236265856798
70-71	19.21746813075855	25.43192513658246	23.07531418474243	32.27529254791656
72-73	18.777023299087293	25.502421503964918	21.871788306822403	33.84876689012539
74-75	20.215647031247183	25.291646382142407	23.763365247651503	30.72934133895891
76	20.374751175605113	24.51316966217581	24.20231947609843	30.909759686120648
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.5
3	0.5
4	0.0
5	0.5
6	0.5
7	0.0
8	0.0
9	0.5
10	0.5
11	0.5
12	1.0
13	0.5
14	0.5
15	0.5
16	1.5
17	3.0
18	4.0
19	9.5
20	19.0
21	24.0
22	32.5
23	57.5
24	97.0
25	120.0
26	176.5
27	272.5
28	420.0
29	528.0
30	596.0
31	848.0
32	1283.0
33	1534.0
34	1803.0
35	2439.0
36	3157.0
37	3508.0
38	4053.0
39	5102.5
40	5958.5
41	6868.0
42	7426.0
43	7812.5
44	8330.0
45	8598.0
46	8735.0
47	8570.5
48	8255.0
49	7759.0
50	7414.0
51	7002.0
52	6172.0
53	5331.0
54	4908.0
55	4529.0
56	3764.5
57	3126.5
58	2874.0
59	2721.0
60	2420.5
61	2193.5
62	2114.0
63	2059.5
64	1895.5
65	1812.0
66	1772.0
67	1706.0
68	1663.5
69	1544.0
70	1444.0
71	1421.0
72	1363.5
73	1208.0
74	1037.0
75	964.0
76	844.5
77	669.5
78	526.5
79	439.0
80	370.5
81	257.5
82	172.5
83	132.0
84	98.0
85	56.5
86	34.5
87	20.0
88	12.5
89	6.5
90	7.5
91	4.5
92	2.0
93	2.0
94	1.0
95	1.5
96	3.0
97	2.0
98	1.5
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	7.211989210864141E-4
5	0.0
6	0.0
7	0.0
8	0.002163596763259242
9	0.0014423978421728282
10-11	0.00360599460543207
12-13	0.003245395144888863
14-15	0.002163596763259242
16-17	0.004687792987061692
18-19	0.0028847956843456564
20-21	0.002163596763259242
22-23	0.002524196223802449
24-25	0.0014423978421728282
26-27	0.001802997302716035
28-29	0.001802997302716035
30-31	0.00360599460543207
32-33	0.002163596763259242
34-35	0.001802997302716035
36-37	0.004327193526518484
38-39	0.003245395144888863
40-41	0.003966594065975278
42-43	0.001802997302716035
44-45	0.0028847956843456564
46-47	0.002163596763259242
48-49	0.003245395144888863
50-51	0.002524196223802449
52-53	0.00360599460543207
54-55	0.003966594065975278
56-57	0.002163596763259242
58-59	0.003966594065975278
60-61	0.003245395144888863
62-63	0.00360599460543207
64-65	0.002163596763259242
66-67	0.00613019082923452
68-69	0.024881362777481285
70-71	0.003966594065975278
72-73	0.003245395144888863
74-75	0.003966594065975278
76	0.004327193526518484
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	138658.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	93.16774444900696
#Duplication Level	Percentage of deduplicated	Percentage of total
1	95.3903457364524	88.8730335447622
2	3.2087703711803903	5.979077958753596
3	0.7817938668968701	2.185139136085457
4	0.31658447855911076	1.1798184717966942
5	0.14183486394397993	0.6607217178946196
6	0.06886763476251095	0.3849745317816701
7	0.03891777673462464	0.2538117034134512
8	0.020954722185382177	0.15618433612540988
9	0.01197398261311808	0.10040300569222542
>10	0.019956566671626794	0.2268355936946961
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0028847956843456564	0.0
10	0.0	0.0	0.0	0.005048392447604898	0.0
11	0.0	0.0	0.0	0.005769591368691313	0.0
12	0.0	0.0	0.0	0.005769591368691313	0.0
13	0.0	0.0	0.0	0.005769591368691313	0.0
14	0.0	0.0	0.0	0.005769591368691313	0.0
15	0.0	0.0	0.0	0.007211989210864141	0.0
16	0.0	0.0	0.0	0.007933188131950555	0.0
17	0.0	0.0	0.0	0.007933188131950555	0.0
18	0.0	0.0	0.0	0.008654387053036968	0.0
19	0.0	0.0	0.0	0.010096784895209796	0.0
20	0.0	0.0	0.0	0.013702779500641867	0.0
21	0.0	0.0	0.0	0.016587575184987522	0.0
22	0.0	0.0	0.0	0.020193569790419592	0.0
23	0.0	0.0	0.0	0.02307836547476525	0.0
24	0.0	0.0	0.0	0.03029035468562939	0.0
25	0.0	0.0	0.0	0.033175150369975044	0.0
26	0.0	0.0	0.0	0.03533874713323429	0.0
27	0.0	0.0	0.0	0.059138311529085955	0.0
28	0.0	0.0	0.0	0.08654387053036969	0.0
29	0.0	0.0	0.0	0.11539182737382625	0.0
30	0.0	0.0	0.0	0.1485669777438013	0.0
31	0.0	0.0	0.0	0.1687605475342209	0.0
32	0.0	0.0	0.0	0.20121449898310953	0.0
33	0.0	0.0	0.0	0.2416016385639487	0.0
34	0.0	0.0	0.0	0.297855154408689	0.0
35	0.0	0.0	0.0	0.3995442022818734	0.0
36	0.0	0.0	0.0	0.4839244760489838	0.0
37	0.0	0.0	0.0	0.7334593027448831	0.0
38	0.0	0.0	0.0	1.0140056830474982	0.0
39	0.0	0.0	0.0	1.381095933880483	0.0
40	0.0	0.0	0.0	1.7618889642141096	0.0
41	0.0	0.0	0.0	2.2934125690547966	0.0
42	0.0	0.0	0.0	2.798973012736373	0.0
43	0.0	0.0	0.0	3.370883757157899	0.0
44	0.0	0.0	0.0	3.975248453028314	0.0
45	0.0	0.0	0.0	4.3574838812041135	0.0
46	0.0	0.0	0.0	4.842129556174184	0.0
47	0.0	0.0	0.0	5.429185477938525	0.0
48	0.0	0.0	0.0	5.879934803617534	0.0
49	0.0	0.0	0.0	6.280200204820494	0.0
50	0.0	0.0	0.0	6.888170895296341	0.0
51	0.0	0.0	0.0	7.494699187930014	0.0
52	0.0	0.0	0.0	8.075985518325664	0.0
53	0.0	0.0	0.0	8.75679729983124	0.0
54	0.0	0.0	0.0	9.447705866232024	0.0
55	0.0	0.0	0.0	9.897733992989947	0.0
56	0.0	0.0	0.0	10.36579209277503	0.0
57	0.0	0.0	7.211989210864141E-4	10.81221422492752	0.0
58	0.0	0.0	7.211989210864141E-4	11.24421237865828	0.0
59	0.0	0.0	7.211989210864141E-4	11.721646064417488	0.0
60	0.0	0.0	7.211989210864141E-4	12.034646396168991	0.0
61	0.0	0.0	7.211989210864141E-4	12.346204330078322	0.0
62	0.0	0.0	7.211989210864141E-4	12.576987984825974	0.0
63	0.0	0.0	7.211989210864141E-4	12.882776327366614	0.0
64	0.0	0.0	0.0014423978421728282	13.221739820277229	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTGG	15	0.0022214046	69.99495	45
GCCGTAT	70	0.0	59.995678	54
TCGGTGG	130	0.0	59.226498	45
CTGTCGT	25	2.3845349E-4	55.99596	52
TCTCGGT	125	0.0	53.19616	43
TACGGCC	20	0.0069214078	52.534103	67
ACGTTAC	20	0.006941194	52.496216	41
CGACTTT	20	0.006941194	52.496216	25
TGGGACC	20	0.006941194	52.496216	4
GTAACAC	20	0.006941194	52.496216	18
CTCGGTG	145	0.0	50.686	44
ATCTCGG	105	0.0	49.99639	42
GTGTACA	30	5.8635196E-4	46.6633	36
CTCTCGG	60	4.8894435E-9	46.6633	42
TAGATCT	85	1.8189894E-12	45.290848	39
CGGTGGT	140	0.0	44.996758	46
CTGTGTA	55	1.3021963E-7	44.542244	34
CGCCGTA	80	2.910383E-11	43.746845	53
TGCCGTA	40	4.575777E-5	43.746845	53
CCGTATC	100	0.0	41.99697	55
>>END_MODULE